##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633333.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	748839
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4861752659784	33.0	33.0	33.0	27.0	33.0
2	31.675097050233763	33.0	33.0	33.0	27.0	33.0
3	31.405459651540585	33.0	33.0	33.0	27.0	33.0
4	31.717303719491106	33.0	33.0	33.0	27.0	33.0
5	31.825849081044122	33.0	33.0	33.0	33.0	33.0
6	34.857065403911925	37.0	37.0	37.0	27.0	37.0
7	35.02868306805602	37.0	37.0	37.0	33.0	37.0
8	35.1493325000434	37.0	37.0	37.0	33.0	37.0
9	35.291658153488264	37.0	37.0	37.0	33.0	37.0
10	35.3158622881554	37.0	37.0	37.0	33.0	37.0
11	35.307107402258694	37.0	37.0	37.0	33.0	37.0
12	35.32037727735869	37.0	37.0	37.0	33.0	37.0
13	35.30446865080478	37.0	37.0	37.0	33.0	37.0
14	35.30896627980113	37.0	37.0	37.0	33.0	37.0
15	35.30510563685919	37.0	37.0	37.0	33.0	37.0
16	35.283474819019844	37.0	37.0	37.0	33.0	37.0
17	35.29436901657098	37.0	37.0	37.0	33.0	37.0
18	35.27355012225592	37.0	37.0	37.0	33.0	37.0
19	35.255718518933975	37.0	37.0	37.0	33.0	37.0
20	35.262168503510104	37.0	37.0	37.0	33.0	37.0
21	35.26277878155385	37.0	37.0	37.0	33.0	37.0
22	35.161638215958305	37.0	37.0	37.0	33.0	37.0
23	35.2066011519165	37.0	37.0	37.0	33.0	37.0
24	35.182518538697906	37.0	37.0	37.0	33.0	37.0
25	35.208593569512274	37.0	37.0	37.0	33.0	37.0
26	35.09726523324773	37.0	37.0	37.0	33.0	37.0
27	35.11097178432213	37.0	37.0	37.0	33.0	37.0
28	35.1303778248729	37.0	37.0	37.0	33.0	37.0
29	35.11807477975907	37.0	37.0	37.0	33.0	37.0
30	35.12060269296872	37.0	37.0	37.0	33.0	37.0
31	35.11241134609709	37.0	37.0	37.0	33.0	37.0
32	35.10253472375237	37.0	37.0	37.0	33.0	37.0
33	35.09692604151226	37.0	37.0	37.0	33.0	37.0
34	35.064804317082846	37.0	37.0	37.0	33.0	37.0
35	35.0070175298028	37.0	37.0	37.0	27.0	37.0
36	35.030937224156325	37.0	37.0	37.0	27.0	37.0
37	35.032920293948365	37.0	37.0	37.0	27.0	37.0
38	35.020410261751856	37.0	37.0	37.0	27.0	37.0
39	34.915131289903435	37.0	37.0	37.0	27.0	37.0
40	34.69758653061606	37.0	37.0	37.0	27.0	37.0
41	34.87712846152511	37.0	37.0	37.0	27.0	37.0
42	34.9167030563312	37.0	37.0	37.0	27.0	37.0
43	34.53887951882848	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	22.0
16	37.0
17	23.0
18	27.0
19	33.0
20	73.0
21	217.0
22	563.0
23	1267.0
24	2503.0
25	4231.0
26	6690.0
27	9760.0
28	13453.0
29	17676.0
30	22575.0
31	28590.0
32	35929.0
33	46458.0
34	67183.0
35	129547.0
36	361978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.42537848589617	21.049117367017477	13.731122444210303	24.794381702876052
2	15.917039577265607	21.78398828052492	38.43683355167132	23.862138590538155
3	17.67816580065942	27.357415946551928	29.9550370640418	25.00938118874685
4	12.539811628400766	18.12405603874798	39.61158540086721	29.724546931984047
5	13.994329889335358	37.02130898631081	35.555573360896	13.428787763457834
6	29.30830258573605	40.21104670029205	17.344182127266343	13.136468586705554
7	26.48820373938857	32.37518345064827	23.630179517893698	17.506433292069456
8	24.43956578116257	36.000395278557875	20.542466404661084	19.01757253561847
9	25.905034326470712	15.33720866568114	20.43550082193903	38.32225618590912
10	14.920430159219805	28.05516272523199	33.980468431799096	23.04393868374911
11	33.91556796587785	23.58464235970616	24.15031802563702	18.349471648778977
12	22.806504468917886	26.0433818217267	30.202753862979893	20.947359846375523
13	27.65748044639769	22.518458573872355	26.161297688822298	23.66276329090766
14	21.69371520446985	21.097325326271736	28.128209134406728	29.080750334851686
15	23.921163294112617	28.66891281036378	24.683543458607257	22.726380436916347
16	22.75816296961029	28.53016469494778	25.30116620528578	23.410506130156147
17	22.275282136747684	27.694871661331742	26.564989270056717	23.46485693186386
18	22.784470360117464	27.50858328692816	27.73680323807921	21.97014311487516
19	24.512478650283974	26.633361777364694	27.53569191775535	21.31846765459598
20	23.857064068511388	26.40487474610697	27.78247393632009	21.955587249061548
21	22.857516769292197	27.114239509427264	27.82720985418762	22.201033867092924
22	22.73479346027651	26.91272756894339	27.621958792210343	22.730520178569762
23	22.54730322539291	26.889892219822954	28.116858229873177	22.445946324910963
24	22.877414237239247	27.570145251516013	26.52479371400261	23.02764679724213
25	22.866330412812367	27.075646434013183	27.660017707411072	22.398005445763374
26	22.941914083000484	27.29972664351082	27.677110834238068	22.081248439250626
27	22.890901782626173	27.208118166922397	26.9931186810516	22.907861369399832
28	22.30532864874826	26.98043237598469	27.93871579872309	22.775523176543956
29	23.849986445684586	26.917134390703474	26.873199713155966	22.35967945045597
30	23.360161530048515	26.607187926910857	27.613145148690172	22.419505394350455
31	22.88783036139945	26.77731795486079	27.632775536530552	22.702076147209212
32	21.95772388991492	26.70120012445933	27.954473525016727	23.386602460609023
33	21.991776603515575	26.823789893421683	28.356963245771123	22.827470257291623
34	22.88662850091942	26.63082451635131	27.934041896856332	22.54850508587293
35	22.63864462187466	27.29118008009732	27.484412537274366	22.585762760753646
36	23.601601946479818	26.96307216905102	27.10315568500038	22.332170199468777
37	22.846299404811983	26.61146120861761	27.283568297057176	23.258671089513232
38	22.93136441878695	26.984839197744776	27.23816467892297	22.8456317045453
39	22.49936234624532	26.78319371720757	27.585902977809653	23.13154095873746
40	22.57561371670012	27.612343908370157	27.77205781215989	22.039984562769835
41	22.657473769395022	26.454017485734582	27.694604581225068	23.193904163645325
42	22.295580224854742	27.405089745592843	27.544238481168847	22.755091548383565
43	23.020035014201984	26.40300518536027	27.439943699513513	23.13701610092423
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	25.0
2	38.0
3	171.5
4	305.0
5	305.0
6	359.5
7	414.0
8	425.0
9	436.0
10	601.5
11	767.0
12	767.0
13	1220.0
14	1673.0
15	3014.5
16	4356.0
17	5003.0
18	5650.0
19	5650.0
20	5433.0
21	5216.0
22	5686.0
23	6156.0
24	8103.0
25	10050.0
26	10050.0
27	13040.5
28	16031.0
29	18931.5
30	21832.0
31	25917.0
32	30002.0
33	30002.0
34	35967.5
35	41933.0
36	45324.5
37	48716.0
38	51586.5
39	54457.0
40	54457.0
41	56340.5
42	58224.0
43	59387.0
44	60550.0
45	59113.5
46	57677.0
47	57677.0
48	55921.5
49	54166.0
50	54013.5
51	53861.0
52	51099.5
53	48338.0
54	48338.0
55	46500.5
56	44663.0
57	38817.5
58	32972.0
59	30741.0
60	28510.0
61	28510.0
62	24853.0
63	21196.0
64	17697.0
65	14198.0
66	12144.0
67	10090.0
68	10090.0
69	8292.5
70	6495.0
71	5335.5
72	4176.0
73	3356.5
74	2537.0
75	2537.0
76	1929.5
77	1322.0
78	1044.0
79	766.0
80	620.5
81	475.0
82	475.0
83	371.5
84	268.0
85	221.5
86	175.0
87	130.0
88	85.0
89	85.0
90	63.0
91	41.0
92	25.0
93	9.0
94	5.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	748839.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.79334456348249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.58286335608038	54.9962091657476
2	7.627960579629269	9.579703139866446
3	1.858066818160532	3.5002268980418347
4	0.8085196215549867	2.0307860473055497
5	0.4465300654842601	1.4019558129953769
6	0.29174645221566803	1.0991841299491212
7	0.18158549920057376	0.7981652573323523
8	0.15237302303366426	0.7654409390025867
9	0.11719392522504565	0.6623098674662964
>10	0.7621484294738676	9.398228072861075
>50	0.09130701487355677	4.050390318660558
>100	0.07390456599373357	8.71671950766642
>500	0.005156132510588039	2.0883216945225205
>1k	6.445165638235048E-4	0.9123591485824164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3190	0.4259927701415124	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2265	0.3024682208058074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1308	0.17467038976335367	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	944	0.12606181034908706	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	928	0.12392516949571271	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	854	0.1140432055488563	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	787	0.10509602197535117	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	784	0.10469540181534348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.670801066717946E-4	0.0	0.0
2	0.0	0.0	2.670801066717946E-4	1.335400533358973E-4	0.0
3	0.0	0.0	2.670801066717946E-4	2.670801066717946E-4	0.0
4	0.0	0.0	4.006201600076919E-4	4.006201600076919E-4	0.0
5	0.0	0.0	6.677002666794866E-4	4.006201600076919E-4	0.0
6	0.0	0.0	0.0017360206933666649	4.006201600076919E-4	0.0
7	0.0	0.0	0.0037391214934051247	5.341602133435892E-4	0.0
8	0.0	0.0	0.004807441920092303	5.341602133435892E-4	0.0
9	0.0	0.0	0.0066770026667948654	9.347803733512812E-4	0.0
10	0.0	0.0	0.007077622826802557	9.347803733512812E-4	0.0
11	0.0	0.0	0.008813643520169221	0.0010683204266871784	0.0
12	0.0	0.0	0.01041612416019999	0.0010683204266871784	0.0
13	0.0	0.0	0.011350904533551271	0.0012018604800230757	0.0
14	0.0	0.0	0.012152144853566655	0.0012018604800230757	0.0
15	0.0	0.0	0.013220465280253833	0.0012018604800230757	0.0
16	0.0	0.0	0.013621085440261525	0.0013354005333589731	0.0
17	0.0	0.0	0.013888165546933319	0.0013354005333589731	0.0
18	0.0	0.0	0.014422325760276909	0.0013354005333589731	0.0
19	0.0	0.0	0.014555865813612807	0.0013354005333589731	0.0
20	0.0	0.0	0.014555865813612807	0.0014689405866948704	1.335400533358973E-4
21	0.0	0.0	0.015223566080292293	0.0014689405866948704	2.670801066717946E-4
22	0.0	0.0	0.016158346453643575	0.0014689405866948704	2.670801066717946E-4
23	0.0	0.0	0.016158346453643575	0.0017360206933666649	4.006201600076919E-4
24	0.0	0.0	0.01629188650697947	0.0020031008000384594	4.006201600076919E-4
25	1.335400533358973E-4	0.0	0.016558966613651267	0.0022701809067102543	4.006201600076919E-4
26	1.335400533358973E-4	0.0	0.016558966613651267	0.0026708010667179463	4.006201600076919E-4
27	1.335400533358973E-4	0.0	0.016558966613651267	0.004406821760084611	4.006201600076919E-4
28	1.335400533358973E-4	0.0	0.016558966613651267	0.010683204266871785	4.006201600076919E-4
29	1.335400533358973E-4	0.0	0.016558966613651267	0.026040310400499974	4.006201600076919E-4
30	1.335400533358973E-4	0.0	0.016558966613651267	0.04620485845422047	4.006201600076919E-4
31	1.335400533358973E-4	0.0	0.016558966613651267	0.10696558272205374	4.006201600076919E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTTCG	125	0.0	25.160002	21
GCCGGCA	135	0.0	23.296297	15
GCCGCTC	130	0.0	22.76923	27
GGTATCA	715	0.0	21.993008	1
TTCGCCG	135	0.0	21.925926	24
GCTTCGC	145	0.0	21.689655	22
GTATCAA	1680	0.0	21.473213	1
TGCTCGC	165	0.0	21.30303	10
CCGCTCT	140	0.0	21.142859	28
CGCTCTC	150	0.0	20.966665	29
AGGACCG	100	1.2880264E-8	20.35	5
TAGGACC	200	0.0	20.35	4
CGCCGCT	165	0.0	20.181818	26
CCGTTTA	55	5.140794E-4	20.181818	27
GCTCGCC	175	0.0	20.085714	11
TCGCCGC	170	0.0	19.588234	25
CGTTAGA	85	1.2441233E-6	19.588234	2
AACGATT	105	2.2562745E-8	19.38095	22
TTAACGG	210	0.0	19.38095	35
CTTCGCC	165	0.0	19.060606	23
>>END_MODULE