FastQCFastQC Report
Fri 10 Feb 2017
ERR1633332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806414
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT221832.7508202982587107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165262.0493195802652235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89431.1089837230008408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.5074316666129308No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA39640.49155892630832304No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA37490.4648976828279271No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG34290.42521583206640756No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG31210.38702205070844503No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA26430.32774728613342524No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA25080.31100650534340923No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT24090.2987299327640641No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22490.27888900738330435No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG21200.2628922612950668No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC20950.25979211670432306No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT20640.2559479374118009No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20620.2556999258445414No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19380.24032320867445256No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT18490.22928669393140494No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18310.22705458982606946No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC17800.22073029486095233No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT17390.21564605773213263No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG16950.21018980325242367No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16510.20473354877271477No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC16370.20299746780189828No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA16200.20088936948019254No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16000.1984092538075976No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC15920.1974172075385596No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC14620.18129645566669228No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA14550.18042841518128405No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14410.17869233421046757No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA14400.17856832842683784No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA14350.1779482995086891No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14120.17509616648520487No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC13950.17298806816349915No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13900.1723680392453504No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13810.17125198719268267No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13710.17001192935638518No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.16877187152008769No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13390.16604374428023322No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13260.1644316690930465No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13230.16405965174215725No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13200.163687634391268No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT12960.16071149558415404No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG12940.16046348401689453No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA12830.1590994203969673No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT12490.15488322375355587No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12280.15227910229733116No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.15103904446103364No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT12130.15041901554288492No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG12090.14992299240836593No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA11850.14694685360125195No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG11530.14297866852510002No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11490.14248264539058103No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11470.14223463382332152No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA11450.141986622256062No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.13801843717991005No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA11040.13690238512724232No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA10950.1357863330745746No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG10910.13529030994005561No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT10870.1347942868055366No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10780.13367823475286886No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10700.1326861884838309No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10650.13206615956568216No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10600.1314461306475334No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10580.1311981190802739No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10550.13082610172938464No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG10430.12933803232582766No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10420.12921402654219794No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10010.12412978941337824No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9890.12264172000982125No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG9720.12053362168811554No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9700.12028561012085603No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC9420.11681344817922308No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC9140.11334128623759013No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8860.10986912429595717No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8780.10887707802691916No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC8630.10701699127247295No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8620.10689298548884318No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC8490.10528091030165647No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8300.10292480041269125No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC8290.1028007946290615No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG8180.10143673100913428No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG8150.10106471365824501No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT8100.10044468474009627No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA8080.10019667317283677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTG200.001841610637.037
CTAATGC200.001841610637.034
TAGTACA200.001841610637.04
AGCTTCT401.5992846E-632.37537
CCTGGTT303.5985347E-430.83333232
TGTCAAC250.00549538329.62
GTACTGA250.00549538329.66
ATAAGTC250.00549538329.65
TATACCT250.00549538329.632
TAATGCC250.00549538329.635
AAGCTTC454.006306E-628.77777936
GGCCGCT358.867092E-426.4285729
GCTAATG358.867092E-426.4285733
TCCTGGT451.3227336E-424.66666831
TATAGTG451.3227336E-424.6666685
CCTATAG551.9017454E-523.5454563
GGTATCA39050.023.498081
AGCAACG400.001930690523.1252
GAGTCGT400.001930690523.12511
TAGCAAC400.001930690523.1251
Produced by FastQC (version 0.11.5)