FastQCFastQC Report
Fri 10 Feb 2017
ERR1633331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947975
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68050.7178459347556634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48300.5095071072549382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26300.2774334766212189No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.12679659273715024No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11170.11783011155357473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.027.2631571
GCCGCTC1700.023.94117727
GTCGACA400.00193093923.12522
TCGGGTA400.00193093923.12525
AGTTCCG400.00193093923.12532
TTCGCCG1700.022.85294224
GTATCAA33150.022.601811
CCGGCAG2050.020.75609616
TTAACGG1251.8189894E-1120.7235
TCGTTTA450.003825320920.55555529
CTACCGA450.003825320920.55555525
AGCTTCG2000.020.3521
CCGTCCG1101.7535058E-920.18181828
GCCGGCA2150.019.79069715
CCGCTCT2000.019.42500128
GCCGTCC1153.0486262E-919.30434827
CGTCCGC1254.129106E-1019.2429
CGAGTCG1355.638867E-1119.18518621
CGCTCTC2100.018.50000229
CGACATC500.007033994418.524
Produced by FastQC (version 0.11.5)