FastQCFastQC Report
Fri 10 Feb 2017
ERR1633330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940958
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23470.2494266481607043No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC19590.20819207658577749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16700.17747869724259743No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15760.16748887835588835No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA14780.15707396079314911No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG14640.15558611542704348No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14120.15005983263865125No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13790.1465527685614023No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13240.14070766176598742No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT11600.12327861604875032No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10870.11552056521119965No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10820.11498919186616194No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10720.1139264451760865No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG10070.10701859169059617No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT9990.10616839433853582No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9990.10616839433853582No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT9940.10563702099349812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9880.10499937297945286No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9620.10223623158525674No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9500.1009609355571662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.023.7614671
GTATCAA14550.022.6323031
CGCTATC450.003825300420.55555512
CGGCGTC555.1421433E-420.18181824
ACACCGT555.1421433E-420.1818186
GCCGAGT1403.6379788E-1219.82142812
TTACGGG500.007033956218.529
GGTTCGC500.007033956218.523
GATAGGG701.21889956E-418.57
CCGAGTT1451.546141E-1017.86206813
TTTGCCG1351.1514203E-917.8148169
CATAATA3450.017.6956522
TTCGCCG650.001579645917.07692324
ACGTAGA1201.0405711E-716.95833226
GACGTAG1107.803901E-716.81818225
TGCGGTA1005.8755104E-616.65000236
GTGACAC4300.016.34883724
TTGCCGA1708.54925E-1116.3235310
ATATAGC3450.016.0869566
TGTTTAC2550.015.96078614
Produced by FastQC (version 0.11.5)