FastQCFastQC Report
Fri 10 Feb 2017
ERR1633329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843624
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43520.5158696291238751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31270.37066275971285784No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC18340.2173954273467801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16880.2000891392373854No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15670.1857462566261747No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG13960.16547656301859598No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11780.13963566707443126No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11470.13596104425668307No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11370.13477568205740947No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10770.1276635088617678No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10710.12695229154220364No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG9140.10834210501360796No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9000.10668259793462491No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8550.10134846803789366No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8520.10099285937811159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.025.4790421
GTATCAA22650.022.6247231
GCCGGCA1800.021.58333415
AATATAG2350.021.2553185
TTCGCCG1750.021.14285924
ATATAGC2300.020.9130446
CCGTCCG909.47075E-820.55555728
GCTTCGC1800.020.55555722
CCGGCAG2000.019.42516
CGCTCTC2000.019.42529
TAGCAAG2200.019.3409089
TGCTCGC2200.019.34090810
TAGGACC2500.019.2400024
CGGCAGC2250.018.91111217
CGAGTCG1205.167749E-918.521
CGCTCAC701.21870944E-418.510
AGCTTCG2000.018.49999821
GTTATCG500.00703337118.49999811
CCGCTCT2050.018.04878228
CAAGCAG5100.017.7745132
Produced by FastQC (version 0.11.5)