##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	843624
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.838845267559957	33.0	33.0	33.0	27.0	33.0
2	31.94825182782851	33.0	33.0	33.0	33.0	33.0
3	31.66749642020616	33.0	33.0	33.0	27.0	33.0
4	31.956601519160195	33.0	33.0	33.0	33.0	33.0
5	32.048162451518685	33.0	33.0	33.0	33.0	33.0
6	35.208477947521644	37.0	37.0	37.0	33.0	37.0
7	35.3578561065119	37.0	37.0	37.0	33.0	37.0
8	35.48802428570074	37.0	37.0	37.0	33.0	37.0
9	35.605771054403384	37.0	37.0	37.0	33.0	37.0
10	35.60519259765014	37.0	37.0	37.0	33.0	37.0
11	35.62889747091121	37.0	37.0	37.0	33.0	37.0
12	35.6153701174931	37.0	37.0	37.0	33.0	37.0
13	35.62249177358634	37.0	37.0	37.0	33.0	37.0
14	35.59795833214797	37.0	37.0	37.0	33.0	37.0
15	35.6107934340417	37.0	37.0	37.0	33.0	37.0
16	35.60017970090941	37.0	37.0	37.0	33.0	37.0
17	35.60098337648052	37.0	37.0	37.0	33.0	37.0
18	35.58505447924668	37.0	37.0	37.0	33.0	37.0
19	35.57323049130893	37.0	37.0	37.0	33.0	37.0
20	35.576228272310885	37.0	37.0	37.0	33.0	37.0
21	35.57450119958654	37.0	37.0	37.0	33.0	37.0
22	35.472134505419476	37.0	37.0	37.0	33.0	37.0
23	35.53716584639602	37.0	37.0	37.0	33.0	37.0
24	35.522661754525714	37.0	37.0	37.0	33.0	37.0
25	35.54009606175263	37.0	37.0	37.0	33.0	37.0
26	35.43576522242137	37.0	37.0	37.0	33.0	37.0
27	35.441362502726335	37.0	37.0	37.0	33.0	37.0
28	35.46883919850549	37.0	37.0	37.0	33.0	37.0
29	35.472934624903985	37.0	37.0	37.0	33.0	37.0
30	35.470210662570054	37.0	37.0	37.0	33.0	37.0
31	35.4658995002513	37.0	37.0	37.0	33.0	37.0
32	35.453715162204965	37.0	37.0	37.0	33.0	37.0
33	35.45348164585171	37.0	37.0	37.0	33.0	37.0
34	35.42921965235698	37.0	37.0	37.0	33.0	37.0
35	35.36400695096393	37.0	37.0	37.0	33.0	37.0
36	35.384159293713786	37.0	37.0	37.0	33.0	37.0
37	35.374670469308604	37.0	37.0	37.0	33.0	37.0
38	35.36289389585882	37.0	37.0	37.0	33.0	37.0
39	35.2557122604383	37.0	37.0	37.0	33.0	37.0
40	35.0491214095379	37.0	37.0	37.0	27.0	37.0
41	35.22059234919822	37.0	37.0	37.0	33.0	37.0
42	35.251569419551835	37.0	37.0	37.0	33.0	37.0
43	34.91330972091832	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	20.0
16	37.0
17	34.0
18	33.0
19	22.0
20	52.0
21	135.0
22	372.0
23	891.0
24	1861.0
25	3409.0
26	5479.0
27	8203.0
28	11831.0
29	16123.0
30	21185.0
31	27008.0
32	34594.0
33	45503.0
34	65216.0
35	131862.0
36	469753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.439906877945624	20.141911562497036	13.45089755625729	24.967284003300048
2	16.352901292518943	20.983163115321517	38.82239006950964	23.841545522649902
3	17.74807260106398	27.165893810512742	29.71940106018795	25.36663252823533
4	12.447369918352253	17.549405896465725	39.62606564061715	30.377158544564875
5	13.749964439134022	37.28307871753293	35.1024864157492	13.864470427583854
6	29.750339013588995	39.69339421353589	16.983513982532504	13.572752790342618
7	26.99318653807857	31.958431718395875	23.31204422823438	17.736337515291172
8	24.916313428731282	35.41696300721648	20.232354698301613	19.434368865750617
9	26.370397238580225	15.021621006514751	20.113344333494542	38.49463742141049
10	15.813798564289305	27.578992536959596	33.18575573952377	23.421453159227333
11	34.053915014271766	23.62604667482196	23.60364332925569	18.716394981650595
12	23.701672783135617	25.804623860866926	29.236010355324172	21.257693000673285
13	27.6773775994993	22.064806122158686	25.91841863199719	24.339397646344818
14	22.22328904820157	20.699743013475196	27.837638568841093	29.239329369482135
15	24.559045261870217	27.97893374299451	24.774899718357943	22.687121276777333
16	22.96662968336605	27.975496192616617	24.875299896636417	24.18257422738092
17	22.53326126331162	27.12606564061715	25.95480925151489	24.385863844556344
18	23.065133282125686	26.670293874996442	27.82708884526756	22.43748399761031
19	25.295510796278908	25.661432107194678	27.18746740253952	21.855589693986897
20	24.207822442225446	25.905261111585254	27.65805619565114	22.228860250538155
21	23.54141181379382	26.50351341355865	27.495187429470946	22.459887343176582
22	22.999227143846074	26.56835272585891	27.23334091965141	23.199079210643607
23	22.96070287236968	26.492015400225693	27.74126862204015	22.806013105364475
24	23.60222089461656	27.037637620551337	26.140436971921137	23.219704512910965
25	23.240092742738472	26.468071083800364	27.668487383004752	22.62334879045641
26	23.48676661640731	26.73169563691882	27.684015627815235	22.097522118858638
27	23.534892321697818	26.42563511706637	26.52354603472637	23.51592652650944
28	22.30176002579348	26.303779882981043	28.145002987112743	23.249457104112732
29	24.51127516523949	26.07844252889913	26.91933847306383	22.490943832797548
30	23.716371274406608	25.837814002446585	27.62379922809214	22.822015495054668
31	22.954183380273676	26.19816411102576	27.7059448285018	23.14170768019876
32	21.973770305254472	26.03837728656368	27.987349814609352	24.00050259357249
33	22.346922325585805	26.323338359269062	28.14606981309209	23.183669502053046
34	23.250049785212372	26.288607246830342	27.883867694612768	22.577475273344525
35	23.341322674556437	26.76168530056044	27.170161114430126	22.726830910452996
36	24.18375958958019	26.523783107166228	26.615530141390003	22.67692716186358
37	23.165177851744378	26.08152447061724	27.068101429072666	23.685196248565713
38	23.677491394270433	26.39777910538344	26.890889780281263	23.033839720064865
39	22.56988895526917	26.494149052184383	27.357448341915358	23.578513650631088
40	23.000649578485202	27.00255089945284	27.653196210634125	22.34360331142784
41	22.696130029491812	25.893051880932738	27.632807980806618	23.778010108768836
42	22.31835509658331	27.13863047992945	27.28668221861872	23.256332204868517
43	23.425246318265007	25.554038291940483	27.37961461504177	23.641100774752733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	53.0
2	75.0
3	240.5
4	406.0
5	406.0
6	529.5
7	653.0
8	612.0
9	571.0
10	803.5
11	1036.0
12	1036.0
13	1661.5
14	2287.0
15	3997.5
16	5708.0
17	6389.5
18	7071.0
19	7071.0
20	6289.5
21	5508.0
22	5698.5
23	5889.0
24	7724.5
25	9560.0
26	9560.0
27	12363.0
28	15166.0
29	17957.5
30	20749.0
31	25152.5
32	29556.0
33	29556.0
34	36845.0
35	44134.0
36	48204.0
37	52274.0
38	56037.0
39	59800.0
40	59800.0
41	62074.5
42	64349.0
43	65904.5
44	67460.0
45	65882.5
46	64305.0
47	64305.0
48	61458.5
49	58612.0
50	60590.0
51	62568.0
52	59735.5
53	56903.0
54	56903.0
55	56360.0
56	55817.0
57	48042.0
58	40267.0
59	36585.0
60	32903.0
61	32903.0
62	29771.5
63	26640.0
64	21226.0
65	15812.0
66	13190.5
67	10569.0
68	10569.0
69	8999.5
70	7430.0
71	6477.5
72	5525.0
73	5928.5
74	6332.0
75	6332.0
76	5381.0
77	4430.0
78	3243.5
79	2057.0
80	1283.0
81	509.0
82	509.0
83	405.0
84	301.0
85	236.5
86	172.0
87	144.0
88	116.0
89	116.0
90	86.0
91	56.0
92	35.0
93	14.0
94	8.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	843624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.53947804855089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.33846909493485	43.635011635689494
2	7.74638333800774	7.829963413338051
3	1.9716215253014264	2.9893416839406566
4	0.8755860611865538	1.770066500758199
5	0.5279690481100505	1.3341640058636104
6	0.3615584698058042	1.0963785808810855
7	0.26154020278856904	0.9252673736352505
8	0.19421466869896697	0.785240638833442
9	0.15358336288273086	0.6985820697330962
>10	1.2342479277130463	12.438475419778776
>50	0.1767008423133479	6.2631698556491475
>100	0.1452658577361928	14.40421779748075
>500	0.010240052266530828	3.4742904484194517
>1k	0.002619548254228816	2.355830575998973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4352	0.5158696291238751	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3127	0.37066275971285784	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1834	0.2173954273467801	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1688	0.2000891392373854	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1567	0.1857462566261747	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1396	0.16547656301859598	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1178	0.13963566707443126	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1147	0.13596104425668307	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1137	0.13477568205740947	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1077	0.1276635088617678	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1071	0.12695229154220364	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	914	0.10834210501360796	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	900	0.10668259793462491	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	855	0.10134846803789366	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	852	0.10099285937811159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.3707243985472202E-4	0.0	0.0
2	1.1853621992736101E-4	0.0	2.3707243985472202E-4	0.0	0.0
3	1.1853621992736101E-4	0.0	3.55608659782083E-4	0.0	0.0
4	1.1853621992736101E-4	0.0	3.55608659782083E-4	0.0	0.0
5	1.1853621992736101E-4	0.0	5.926810996368051E-4	0.0	0.0
6	1.1853621992736101E-4	0.0	0.0011853621992736101	0.0	0.0
7	1.1853621992736101E-4	0.0	0.003319014157966108	0.0	0.0
8	1.1853621992736101E-4	0.0	0.004385840137312357	0.0	1.1853621992736101E-4
9	1.1853621992736101E-4	0.0	0.005808274776440689	8.29753539491527E-4	1.1853621992736101E-4
10	2.3707243985472202E-4	0.0	0.006519492096004855	9.482897594188881E-4	1.1853621992736101E-4
11	2.3707243985472202E-4	0.0	0.009719970034043603	0.0011853621992736101	1.1853621992736101E-4
12	2.3707243985472202E-4	0.0	0.010786796013389851	0.0011853621992736101	1.1853621992736101E-4
13	2.3707243985472202E-4	0.0	0.01173508577280874	0.0011853621992736101	1.1853621992736101E-4
14	2.3707243985472202E-4	0.0	0.013276056631864432	0.0011853621992736101	1.1853621992736101E-4
15	2.3707243985472202E-4	0.0	0.014935563710847486	0.0011853621992736101	1.1853621992736101E-4
16	2.3707243985472202E-4	0.0	0.015765317250339014	0.0011853621992736101	1.1853621992736101E-4
17	2.3707243985472202E-4	0.0	0.016239462130048458	0.0011853621992736101	1.1853621992736101E-4
18	2.3707243985472202E-4	0.0	0.0167136070097579	0.001303898419200971	1.1853621992736101E-4
19	2.3707243985472202E-4	0.0	0.017306288109394708	0.001303898419200971	1.1853621992736101E-4
20	2.3707243985472202E-4	0.0	0.017661896769176788	0.001303898419200971	1.1853621992736101E-4
21	3.55608659782083E-4	0.0	0.018136041648886235	0.0015409708590556931	2.3707243985472202E-4
22	3.55608659782083E-4	0.0	0.01861018652859568	0.0023707243985472202	2.3707243985472202E-4
23	3.55608659782083E-4	0.0	0.0188472589684504	0.002607796838401942	2.3707243985472202E-4
24	3.55608659782083E-4	0.0	0.019084331408305122	0.002963405498184025	2.3707243985472202E-4
25	3.55608659782083E-4	0.0	0.019202867628232482	0.003200477938038747	2.3707243985472202E-4
26	3.55608659782083E-4	0.0	0.019202867628232482	0.00355608659782083	2.3707243985472202E-4
27	4.7414487970944404E-4	0.0	0.019202867628232482	0.004385840137312357	2.3707243985472202E-4
28	5.926810996368051E-4	0.0	0.019321403848159845	0.014698491270992764	2.3707243985472202E-4
29	7.11217319564166E-4	0.0	0.019321403848159845	0.031649170720605385	2.3707243985472202E-4
30	7.11217319564166E-4	0.0	0.019321403848159845	0.06021639972309939	2.3707243985472202E-4
31	7.11217319564166E-4	0.0	0.019321403848159845	0.13074545057987919	2.3707243985472202E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	835	0.0	25.479042	1
GTATCAA	2265	0.0	22.624723	1
GCCGGCA	180	0.0	21.583334	15
AATATAG	235	0.0	21.255318	5
TTCGCCG	175	0.0	21.142859	24
ATATAGC	230	0.0	20.913044	6
CCGTCCG	90	9.47075E-8	20.555557	28
GCTTCGC	180	0.0	20.555557	22
CCGGCAG	200	0.0	19.425	16
CGCTCTC	200	0.0	19.425	29
TAGCAAG	220	0.0	19.340908	9
TGCTCGC	220	0.0	19.340908	10
TAGGACC	250	0.0	19.240002	4
CGGCAGC	225	0.0	18.911112	17
CGAGTCG	120	5.167749E-9	18.5	21
CGCTCAC	70	1.21870944E-4	18.5	10
AGCTTCG	200	0.0	18.499998	21
GTTATCG	50	0.007033371	18.499998	11
CCGCTCT	205	0.0	18.048782	28
CAAGCAG	510	0.0	17.77451	32
>>END_MODULE