FastQCFastQC Report
Fri 10 Feb 2017
ERR1633328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182006
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55600.4703867831466169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.31463461268386117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21730.18384001434848893No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA12560.1062600359050631No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11990.10143772535841612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.026.6639671
GTATCAA34150.023.4568081
TTCGCCG753.7409518E-722.224
TTAAGAC957.1449904E-921.4210533
TTAACGG1550.020.29032335
ATGTCCG1403.6379788E-1219.8214329
AACGGCC1600.019.6562537
GCGAACT2800.017.17857228
GTCGCCC2400.016.95833437
ATAAGAC1201.0412441E-716.9583343
CGTGGCG1554.0017767E-1016.70967934
TGTCCGA1005.8780715E-616.65000212
CGAACTA2800.016.51785929
TTCTGCG1801.0913936E-1116.44444518
CGCCGTT803.3826657E-416.187525
CCTCGCG700.00259255215.85714331
CGGTAGG700.00259255215.85714329
ATCAACG49950.015.8518513
TCAACGC50600.015.8310274
TATCAAC50800.015.6958672
Produced by FastQC (version 0.11.5)