##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633328.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1182006
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.851069283912263	33.0	33.0	33.0	27.0	33.0
2	31.959837767320977	33.0	33.0	33.0	33.0	33.0
3	31.67676560017462	33.0	33.0	33.0	27.0	33.0
4	31.96068463273452	33.0	33.0	33.0	33.0	33.0
5	32.061744187423756	33.0	33.0	33.0	33.0	33.0
6	35.23014180977085	37.0	37.0	37.0	33.0	37.0
7	35.382968445168636	37.0	37.0	37.0	33.0	37.0
8	35.52463016262185	37.0	37.0	37.0	33.0	37.0
9	35.62927683954227	37.0	37.0	37.0	33.0	37.0
10	35.63535718092802	37.0	37.0	37.0	33.0	37.0
11	35.660038950732904	37.0	37.0	37.0	33.0	37.0
12	35.6485161665846	37.0	37.0	37.0	33.0	37.0
13	35.64422854029506	37.0	37.0	37.0	33.0	37.0
14	35.625222714605506	37.0	37.0	37.0	33.0	37.0
15	35.64934526559087	37.0	37.0	37.0	33.0	37.0
16	35.63397647727677	37.0	37.0	37.0	33.0	37.0
17	35.63527173296921	37.0	37.0	37.0	33.0	37.0
18	35.62340461892749	37.0	37.0	37.0	33.0	37.0
19	35.61375407569843	37.0	37.0	37.0	33.0	37.0
20	35.60764412363389	37.0	37.0	37.0	33.0	37.0
21	35.613997729283945	37.0	37.0	37.0	33.0	37.0
22	35.526746057126616	37.0	37.0	37.0	33.0	37.0
23	35.56951064546204	37.0	37.0	37.0	33.0	37.0
24	35.554778909751725	37.0	37.0	37.0	33.0	37.0
25	35.57780586562166	37.0	37.0	37.0	33.0	37.0
26	35.4673098106101	37.0	37.0	37.0	33.0	37.0
27	35.485330023705465	37.0	37.0	37.0	33.0	37.0
28	35.50048561513224	37.0	37.0	37.0	33.0	37.0
29	35.5054509029565	37.0	37.0	37.0	33.0	37.0
30	35.51608198266337	37.0	37.0	37.0	33.0	37.0
31	35.51422412407382	37.0	37.0	37.0	33.0	37.0
32	35.50309220088561	37.0	37.0	37.0	33.0	37.0
33	35.494595628110176	37.0	37.0	37.0	33.0	37.0
34	35.478165931475814	37.0	37.0	37.0	33.0	37.0
35	35.42044202821305	37.0	37.0	37.0	33.0	37.0
36	35.451183834938234	37.0	37.0	37.0	33.0	37.0
37	35.456295484117675	37.0	37.0	37.0	33.0	37.0
38	35.440818405321124	37.0	37.0	37.0	33.0	37.0
39	35.33813280135634	37.0	37.0	37.0	33.0	37.0
40	35.140131268369196	37.0	37.0	37.0	33.0	37.0
41	35.30492231003903	37.0	37.0	37.0	33.0	37.0
42	35.3473916376059	37.0	37.0	37.0	33.0	37.0
43	35.01714881311939	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	20.0
16	43.0
17	52.0
18	37.0
19	46.0
20	52.0
21	166.0
22	467.0
23	1121.0
24	2418.0
25	4374.0
26	7431.0
27	11128.0
28	16046.0
29	21782.0
30	28700.0
31	36591.0
32	46812.0
33	63020.0
34	90700.0
35	183243.0
36	667752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81873865276488	20.251504645492492	13.733432825214084	24.196323876528545
2	16.136127904596083	21.74735153628662	38.911646810591485	23.204873748525813
3	18.077996219985344	27.197746881149502	30.064737404040248	24.6595194948249
4	12.420241521616642	17.840264770229595	40.05664945863219	29.68284424952158
5	13.864058219670628	36.841268149231055	35.351427996135385	13.943245634962937
6	29.044015004999974	40.101403884582645	17.389674840906054	13.464906269511323
7	26.487852007519418	32.18858449111087	23.503687798539094	17.81987570283061
8	24.735915046116517	35.08349365400852	20.875782356434737	19.304808943440218
9	26.28413053740844	15.385031886470966	20.39211306879999	37.9387245073206
10	15.131649077923463	28.00924868401683	33.71133479863892	23.14776743942078
11	33.51480449337821	24.078388772984233	24.17119710052233	18.235609633115228
12	23.322301240433635	25.847838335846014	30.117106004538048	20.712754419182307
13	28.3142386756074	22.503439068837213	26.044876252743215	23.13744600281217
14	22.22272983385871	21.238047861009164	28.141058505625182	28.398163799506943
15	23.906900641790312	28.730818625286165	24.77026343351895	22.59201729940457
16	22.667059219665553	28.38471209113998	25.47195191902579	23.47627677016868
17	22.494386661319822	27.414158642172715	26.457141503511828	23.634313192995638
18	23.352757938622982	26.804432464809825	27.915509735145168	21.92729986142202
19	25.060278881833085	26.065603727899862	27.723124924915776	21.150992465351276
20	24.855372984570298	25.986416312607552	27.443007903513177	21.715202799308972
21	23.32670054128321	26.751725456554365	27.521687707169	22.399886294993426
22	22.720020033739253	26.898932831136218	27.789452845417028	22.591594289707498
23	22.867988825775843	26.518816317345255	28.016270644988268	22.596924211890634
24	22.649715822085508	26.941656810540728	27.353583653551674	23.055043713822094
25	23.16434941954609	27.0496934871735	27.60112892827955	22.184828165000855
26	23.211388097860755	26.891572462407126	27.521264697471924	22.37577474226019
27	23.039053947272688	26.73015196200358	27.383871147862195	22.846922942861543
28	22.810374905034323	26.695803574601147	27.825662475486588	22.668159044877946
29	23.458764168709802	26.7860738439568	27.327780062030143	22.427381925303255
30	23.36612504505053	26.6300678676758	27.587169608276103	22.41663747899757
31	23.117226139292015	26.6004571888806	27.58818483154908	22.694131840278306
32	22.1537792532356	26.63133689676702	27.935137385089416	23.279746464907962
33	22.17171486439155	26.798171921293125	28.272022307839386	22.75809090647594
34	23.079747480131235	26.202320461994272	27.81297218457436	22.904959873300136
35	22.75496063471759	26.8298976485737	27.594445375065778	22.820696341642936
36	23.170186953365718	26.753502097282077	27.479132931643324	22.597178017708877
37	23.18626132185454	26.23497681060841	27.605782034947367	22.972979832589683
38	23.3363451623765	26.480576240729743	27.393092759258415	22.789985837635342
39	22.907920941179654	26.442505367993057	27.753750826983957	22.895822863843332
40	22.75563745023291	26.79842572711137	27.84012940712653	22.605807415529195
41	22.685164034700332	25.958074662903574	27.97109320934073	23.385668093055365
42	22.41198437232975	26.774652582135793	27.835560902398125	22.97780214313633
43	22.86587377729047	26.315348653052524	27.74334478843593	23.075432781221078
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	90.0
2	105.0
3	323.5
4	542.0
5	542.0
6	692.5
7	843.0
8	748.5
9	654.0
10	955.0
11	1256.0
12	1256.0
13	2041.0
14	2826.0
15	5000.0
16	7174.0
17	8483.0
18	9792.0
19	9792.0
20	9508.5
21	9225.0
22	10562.0
23	11899.0
24	15543.0
25	19187.0
26	19187.0
27	23524.5
28	27862.0
29	32824.0
30	37786.0
31	43330.5
32	48875.0
33	48875.0
34	55405.5
35	61936.0
36	66526.0
37	71116.0
38	74964.0
39	78812.0
40	78812.0
41	81676.0
42	84540.0
43	85783.5
44	87027.0
45	86502.5
46	85978.0
47	85978.0
48	85523.0
49	85068.0
50	84359.0
51	83650.0
52	82723.5
53	81797.0
54	81797.0
55	76948.0
56	72099.0
57	64424.0
58	56749.0
59	51887.0
60	47025.0
61	47025.0
62	42175.0
63	37325.0
64	31363.0
65	25401.0
66	21233.5
67	17066.0
68	17066.0
69	14298.0
70	11530.0
71	9755.5
72	7981.0
73	5938.5
74	3896.0
75	3896.0
76	2961.5
77	2027.0
78	1550.0
79	1073.0
80	856.0
81	639.0
82	639.0
83	545.0
84	451.0
85	401.5
86	352.0
87	275.5
88	199.0
89	199.0
90	160.0
91	121.0
92	79.0
93	37.0
94	23.5
95	10.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1182006.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.38625964230601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.05029097668093	51.69591181879461
2	7.889612235841669	9.370691214296157
3	1.7889832987578274	3.187230800273443
4	0.777937417866538	1.8479517393154934
5	0.4581564903826622	1.3604100147336227
6	0.28914489426922374	1.0302740259191545
7	0.23045257257762478	0.9580001417232534
8	0.1733063494646137	0.8233612693572617
9	0.12528558346256646	0.6696217970051194
>10	0.9961679391729743	11.950619885028855
>50	0.1336909082512426	5.559466524266093
>100	0.08207559841114316	8.707390708018002
>500	0.0038877894484296957	1.4729370141068037
>1k	8.639532107621547E-4	0.8906886466726542
>5k	1.4399220179369245E-4	0.4754444004896273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5560	0.4703867831466169	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3719	0.31463461268386117	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2173	0.18384001434848893	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1256	0.1062600359050631	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1199	0.10143772535841612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.460193941485915E-5	0.0	0.0
2	0.0	0.0	8.460193941485915E-5	0.0	0.0
3	0.0	0.0	1.692038788297183E-4	0.0	0.0
4	0.0	0.0	1.692038788297183E-4	1.692038788297183E-4	0.0
5	0.0	0.0	1.692038788297183E-4	1.692038788297183E-4	0.0
6	0.0	0.0	4.230096970742957E-4	1.692038788297183E-4	0.0
7	0.0	0.0	0.0021150484853714787	4.230096970742957E-4	0.0
8	0.0	0.0	0.0026226601218606337	4.230096970742957E-4	0.0
9	0.0	0.0	0.0032148736977646477	8.460193941485914E-4	0.0
10	8.460193941485915E-5	0.0	0.0036378833948389434	0.0010152232729783097	0.0
11	8.460193941485915E-5	0.0	0.006260543516699577	0.0010998252123931688	0.0
12	1.692038788297183E-4	0.0	0.006598951274359013	0.0010998252123931688	0.0
13	1.692038788297183E-4	0.0	0.007191164850263027	0.0010998252123931688	0.0
14	1.692038788297183E-4	0.0	0.007444970668507605	0.0010998252123931688	0.0
15	2.538058182445774E-4	0.0	0.008121786183826477	0.0010998252123931688	0.0
16	2.538058182445774E-4	0.0	0.008375592002071056	0.0010998252123931688	0.0
17	2.538058182445774E-4	0.0	0.008713999759730492	0.0010998252123931688	0.0
18	2.538058182445774E-4	0.0	0.00896780557797507	0.0010998252123931688	0.0
19	2.538058182445774E-4	0.0	0.009137009456804788	0.0010998252123931688	0.0
20	2.538058182445774E-4	0.0	0.009306213335634505	0.0013536310306377464	0.0
21	3.384077576594366E-4	0.0	0.009644621093293943	0.0018612426671269012	0.0
22	3.384077576594366E-4	0.0	0.009813824972123662	0.0021996504247863377	0.0
23	3.384077576594366E-4	0.0	0.009983028850953379	0.0026226601218606337	0.0
24	3.384077576594366E-4	0.0	0.010067630790368239	0.0032148736977646477	0.0
25	3.384077576594366E-4	0.0	0.010067630790368239	0.003468679516009225	0.0
26	3.384077576594366E-4	0.0	0.010067630790368239	0.004060893091913239	0.0
27	3.384077576594366E-4	0.0	0.010152232729783097	0.005076116364891549	0.0
28	3.384077576594366E-4	0.0	0.010152232729783097	0.013197902548718027	0.0
29	3.384077576594366E-4	0.0	0.010152232729783097	0.032402542795891054	0.0
30	3.384077576594366E-4	0.0	0.010152232729783097	0.05862914401449739	0.0
31	3.384077576594366E-4	0.0	0.010152232729783097	0.1251262683945767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1235	0.0	26.663967	1
GTATCAA	3415	0.0	23.456808	1
TTCGCCG	75	3.7409518E-7	22.2	24
TTAAGAC	95	7.1449904E-9	21.421053	3
TTAACGG	155	0.0	20.290323	35
ATGTCCG	140	3.6379788E-12	19.82143	29
AACGGCC	160	0.0	19.65625	37
GCGAACT	280	0.0	17.178572	28
GTCGCCC	240	0.0	16.958334	37
ATAAGAC	120	1.0412441E-7	16.958334	3
CGTGGCG	155	4.0017767E-10	16.709679	34
TGTCCGA	100	5.8780715E-6	16.650002	12
CGAACTA	280	0.0	16.517859	29
TTCTGCG	180	1.0913936E-11	16.444445	18
CGCCGTT	80	3.3826657E-4	16.1875	25
CCTCGCG	70	0.002592552	15.857143	31
CGGTAGG	70	0.002592552	15.857143	29
ATCAACG	4995	0.0	15.851851	3
TCAACGC	5060	0.0	15.831027	4
TATCAAC	5080	0.0	15.695867	2
>>END_MODULE