FastQCFastQC Report
Fri 10 Feb 2017
ERR1633327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134047
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38010.33517129360599696No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC26090.2300610115806488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.2289146746122515No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC24220.2135713951890883No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC24180.2132186761218891No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG22700.2001680706355204No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA20910.18438389237835823No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC20030.1766240728999768No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG17510.15440277166643004No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT15700.1384422338756683No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA15680.13826587434206872No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC15430.136061380172074No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG15120.13332780740128056No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG15060.13279872880048182No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14810.13059423463048708No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG14770.13024151556328795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14690.12953607742888965No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT14590.12865427976089175No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA14190.1251270890889002No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT13890.12248169608490653No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG13680.12062992098211098No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG13110.11560367427452302No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC12760.11251738243653041No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG12430.10960745013213738No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG12090.10660933806094455No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC11970.10555118085934709No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT11470.10114219251935767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.026.7610051
TAGACCG358.869324E-426.428575
GAACGGT451.3231969E-424.66666620
GTATCAA23850.024.5890981
GACCGTG502.7020718E-422.1999997
TTAGCGA759.265592E-619.73333427
TAGCGAG759.265592E-619.73333428
CGGCGTA801.6166388E-518.512
ACAACGC500.007034826618.4999983
TTTATAC1002.87484E-718.4999983
TATTAGA6350.018.354332
TTTAGGA4000.017.5749992
TAGGACC4050.017.3580254
GTCGCCC5550.017.33333437
GTGACAC6300.017.32539624
TCGGCGT752.0672356E-417.26666611
ATCCGTA650.00157991617.07692312
TTAACGG6050.016.81818235
GACACAT6450.016.63565826
ATTAGAG6600.016.5378783
Produced by FastQC (version 0.11.5)