##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1134047
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816028788930264	33.0	33.0	33.0	27.0	33.0
2	31.932061898669104	33.0	33.0	33.0	33.0	33.0
3	31.65063440933224	33.0	33.0	33.0	27.0	33.0
4	31.942009458161788	33.0	33.0	33.0	33.0	33.0
5	32.03781765658743	33.0	33.0	33.0	33.0	33.0
6	35.20375169635826	37.0	37.0	37.0	33.0	37.0
7	35.34239674369757	37.0	37.0	37.0	33.0	37.0
8	35.47977112059729	37.0	37.0	37.0	33.0	37.0
9	35.59206717181916	37.0	37.0	37.0	33.0	37.0
10	35.60291151954019	37.0	37.0	37.0	33.0	37.0
11	35.62134638158736	37.0	37.0	37.0	33.0	37.0
12	35.59781737441217	37.0	37.0	37.0	33.0	37.0
13	35.611609571737326	37.0	37.0	37.0	33.0	37.0
14	35.59039881063131	37.0	37.0	37.0	33.0	37.0
15	35.605154812807584	37.0	37.0	37.0	33.0	37.0
16	35.58786893312182	37.0	37.0	37.0	33.0	37.0
17	35.585040126202884	37.0	37.0	37.0	33.0	37.0
18	35.56423763741714	37.0	37.0	37.0	33.0	37.0
19	35.55951737450035	37.0	37.0	37.0	33.0	37.0
20	35.55308730590531	37.0	37.0	37.0	33.0	37.0
21	35.56501891015099	37.0	37.0	37.0	33.0	37.0
22	35.46667025264385	37.0	37.0	37.0	33.0	37.0
23	35.51590189824584	37.0	37.0	37.0	33.0	37.0
24	35.51012965071113	37.0	37.0	37.0	33.0	37.0
25	35.52758659914448	37.0	37.0	37.0	33.0	37.0
26	35.421601573832476	37.0	37.0	37.0	33.0	37.0
27	35.43987859409707	37.0	37.0	37.0	33.0	37.0
28	35.45982573914485	37.0	37.0	37.0	33.0	37.0
29	35.47227760401465	37.0	37.0	37.0	33.0	37.0
30	35.46554066983114	37.0	37.0	37.0	33.0	37.0
31	35.44711198036766	37.0	37.0	37.0	33.0	37.0
32	35.450959263593134	37.0	37.0	37.0	33.0	37.0
33	35.46154348100211	37.0	37.0	37.0	33.0	37.0
34	35.44070219311898	37.0	37.0	37.0	33.0	37.0
35	35.376344190320154	37.0	37.0	37.0	33.0	37.0
36	35.41360366898374	37.0	37.0	37.0	33.0	37.0
37	35.411194597754765	37.0	37.0	37.0	33.0	37.0
38	35.3994005539453	37.0	37.0	37.0	33.0	37.0
39	35.31324539459123	37.0	37.0	37.0	33.0	37.0
40	35.1168937442628	37.0	37.0	37.0	33.0	37.0
41	35.293732093996105	37.0	37.0	37.0	33.0	37.0
42	35.33836516475949	37.0	37.0	37.0	33.0	37.0
43	35.00689389416841	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	35.0
16	45.0
17	47.0
18	35.0
19	31.0
20	76.0
21	167.0
22	493.0
23	1233.0
24	2442.0
25	4585.0
26	7560.0
27	11249.0
28	15741.0
29	21453.0
30	28117.0
31	36144.0
32	46279.0
33	60626.0
34	88150.0
35	177286.0
36	632247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56538485618321	20.16829990291408	13.327754493420466	24.938560747482246
2	16.68960810266241	20.783089237042205	38.898299629556796	23.629003030738584
3	17.769104807825425	27.23255738077875	29.27771071216625	25.720627099229574
4	12.556534252989515	17.511531709003243	39.42138200621314	30.5105520317941
5	13.88143524915634	37.08303095021635	35.205507355515245	13.830026445112061
6	29.852466432167272	39.583191878290755	16.958115492567767	13.6062261969742
7	27.48501605312655	31.626290621111824	23.108301507785832	17.7803918179758
8	24.755764090906286	35.33001718623655	20.163802734807287	19.750415988049877
9	26.45542909597221	15.207570762058362	19.830042317470088	38.50695782449934
10	16.00974210063604	27.218095899023588	33.04704302379002	23.725118976550355
11	34.1378267391034	23.699194125111216	23.37160629145	18.791372844335378
12	24.076691706781112	25.72627060430476	28.707628519805617	21.489409169108512
13	27.572666741325534	22.025013072650427	25.672128227489686	24.73019195853435
14	22.476846197732545	20.512200993433254	27.077272811444324	29.933679997389877
15	24.69262737787764	27.68738861793206	24.734600946874334	22.885383057315966
16	23.437564757016244	27.650705834943352	24.621642665603808	24.2900867424366
17	22.88741119195236	27.02004414279126	25.470813819885773	24.621730845370607
18	23.266143290357455	26.84950447380047	27.322941641748532	22.56141059409354
19	25.723977930367965	25.35062479773766	26.48435205948254	22.44104521241183
20	24.74033263171632	25.710133706980397	27.04764440979959	22.501889251503684
21	23.892748713236752	26.5438734020724	26.85259076563846	22.710787119052387
22	23.332895373824893	26.57491267998593	26.5447551997404	23.54743674644878
23	23.367550022177213	26.49784356380291	27.398599881662754	22.736006532357127
24	23.867352940398415	27.211746955813997	25.09631435028707	23.824585753500514
25	23.637380108584566	26.60771555323545	26.939183296635854	22.815721041544133
26	24.002003444301693	26.716970284300384	26.862731438820436	22.418294832577484
27	23.856859548149238	26.54096346976801	25.73870395142353	23.863473030659225
28	22.8311525007341	26.128193981378196	27.59612255929428	23.444530958593425
29	25.097372507488668	25.908450002513124	26.209672085901204	22.784505404097008
30	24.050590495808375	25.7523718152775	27.180619498133673	23.016418190780453
31	23.562250947271146	26.008181318763686	27.042794522625606	23.386773211339566
32	22.242640737112307	25.836583492571297	27.372586850456816	24.54818891985958
33	22.659466494774907	26.27633598960184	27.630248129045796	23.433949386577453
34	23.617275121754215	26.092569355591085	27.405037004639137	22.885118518015567
35	23.828553843006507	26.733636260225545	26.401903977524743	23.035905919243206
36	24.563708558816348	26.408252920734327	25.937108426723054	23.090930093726275
37	23.392945795015553	25.765422420763866	26.570591871412734	24.271039912807847
38	24.013378634218864	26.165670382268107	26.271750641728254	23.549200341784776
39	22.911484268288703	26.4198044701851	27.031242973174834	23.637468288351364
40	23.354675776224443	26.940241453837448	27.0871489453259	22.61793382461221
41	23.060684433713945	25.477339122628955	27.235555492849944	24.226420950807153
42	22.464148311313377	27.182559453003268	26.877545639642804	23.475746596040555
43	23.930225114126664	25.177439735742873	26.781076974763835	24.11125817536663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	44.0
2	51.0
3	213.0
4	375.0
5	375.0
6	434.5
7	494.0
8	466.5
9	439.0
10	604.5
11	770.0
12	770.0
13	1238.0
14	1706.0
15	3169.0
16	4632.0
17	5344.5
18	6057.0
19	6057.0
20	5490.5
21	4924.0
22	5355.0
23	5786.0
24	7648.5
25	9511.0
26	9511.0
27	12900.0
28	16289.0
29	20273.5
30	24258.0
31	30328.5
32	36399.0
33	36399.0
34	47133.5
35	57868.0
36	64042.0
37	70216.0
38	77016.5
39	83817.0
40	83817.0
41	88492.5
42	93168.0
43	95574.5
44	97981.0
45	94759.5
46	91538.0
47	91538.0
48	87202.0
49	82866.0
50	83502.0
51	84138.0
52	80540.0
53	76942.0
54	76942.0
55	77696.0
56	78450.0
57	66329.0
58	54208.0
59	49049.5
60	43891.0
61	43891.0
62	39961.5
63	36032.0
64	28829.5
65	21627.0
66	17786.0
67	13945.0
68	13945.0
69	11852.5
70	9760.0
71	8545.5
72	7331.0
73	7700.5
74	8070.0
75	8070.0
76	6997.5
77	5925.0
78	4328.5
79	2732.0
80	1738.5
81	745.0
82	745.0
83	586.5
84	428.0
85	385.0
86	342.0
87	261.0
88	180.0
89	180.0
90	136.0
91	92.0
92	58.5
93	25.0
94	13.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1134047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.72101092552763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.20272224979945	35.97225417192851
2	8.820376377319642	7.536307911654765
3	2.3155017813324323	2.967617306951446
4	0.997026247313849	1.7037587681813107
5	0.593712044332972	1.2681989366283122
6	0.42014303996222624	1.0769361240306394
7	0.29986515423795146	0.8967379771269185
8	0.23080428833152553	0.7888154018775785
9	0.1776381858813671	0.6829994591845913
>10	1.4893761504696224	13.118021537865548
>50	0.22977999828372478	6.902521689902128
>100	0.1995308461525696	17.356714027552282
>500	0.015332370272590476	4.358088680087581
>1k	0.00819126631001409	5.37102800702826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3801	0.33517129360599696	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2609	0.2300610115806488	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2596	0.2289146746122515	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	2422	0.2135713951890883	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2418	0.2132186761218891	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	2270	0.2001680706355204	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	2091	0.18438389237835823	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	2003	0.1766240728999768	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1751	0.15440277166643004	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1570	0.1384422338756683	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1568	0.13826587434206872	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1543	0.136061380172074	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1512	0.13332780740128056	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1506	0.13279872880048182	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1481	0.13059423463048708	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1477	0.13024151556328795	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1469	0.12953607742888965	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1459	0.12865427976089175	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1419	0.1251270890889002	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	1389	0.12248169608490653	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1368	0.12062992098211098	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	1311	0.11560367427452302	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	1276	0.11251738243653041	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	1243	0.10960745013213738	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	1209	0.10660933806094455	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	1197	0.10555118085934709	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	1147	0.10114219251935767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.817976679978871E-5	0.0	0.0
2	0.0	0.0	8.817976679978871E-5	0.0	0.0
3	0.0	0.0	8.817976679978871E-5	0.0	0.0
4	0.0	0.0	1.7635953359957743E-4	0.0	0.0
5	8.817976679978871E-5	0.0	6.17258367598521E-4	0.0	0.0
6	8.817976679978871E-5	0.0	9.69977434797676E-4	0.0	8.817976679978871E-5
7	8.817976679978871E-5	0.0	0.0014108762687966194	0.0	8.817976679978871E-5
8	1.7635953359957743E-4	0.0	0.0017635953359957744	0.0	8.817976679978871E-5
9	1.7635953359957743E-4	0.0	0.0021163144031949295	5.290786007987324E-4	8.817976679978871E-5
10	1.7635953359957743E-4	0.0	0.002204494169994718	5.290786007987324E-4	8.817976679978871E-5
11	2.645393003993662E-4	0.0	0.0029981120711928167	6.17258367598521E-4	8.817976679978871E-5
12	2.645393003993662E-4	0.0	0.003086291837992605	7.936179011980985E-4	8.817976679978871E-5
13	2.645393003993662E-4	0.0	0.0033508311383919714	7.936179011980985E-4	8.817976679978871E-5
14	2.645393003993662E-4	0.0	0.0034390109051917602	7.936179011980985E-4	8.817976679978871E-5
15	3.5271906719915486E-4	0.0	0.0035271906719915487	7.936179011980985E-4	8.817976679978871E-5
16	3.5271906719915486E-4	0.0	0.003968089505990493	9.69977434797676E-4	1.7635953359957743E-4
17	3.5271906719915486E-4	0.0	0.003968089505990493	9.69977434797676E-4	1.7635953359957743E-4
18	5.290786007987324E-4	0.0	0.004056269272790281	9.69977434797676E-4	1.7635953359957743E-4
19	5.290786007987324E-4	0.0	0.00414444903959007	9.69977434797676E-4	1.7635953359957743E-4
20	5.290786007987324E-4	0.0	0.004232628806389859	0.0011463369683972534	1.7635953359957743E-4
21	5.290786007987324E-4	0.0	0.004408988339989436	0.0013226965019968308	1.7635953359957743E-4
22	6.17258367598521E-4	0.0	0.004497168106789225	0.0013226965019968308	2.645393003993662E-4
23	6.17258367598521E-4	0.0	0.004497168106789225	0.001587235802396197	2.645393003993662E-4
24	6.17258367598521E-4	0.0	0.004497168106789225	0.001939954869595352	2.645393003993662E-4
25	6.17258367598521E-4	0.0	0.004497168106789225	0.0021163144031949295	2.645393003993662E-4
26	6.17258367598521E-4	0.0	0.004497168106789225	0.002821752537593239	2.645393003993662E-4
27	6.17258367598521E-4	0.0	0.004585347873589014	0.0035271906719915487	2.645393003993662E-4
28	8.817976679978872E-4	0.0	0.004585347873589014	0.011639729217572111	2.645393003993662E-4
29	9.69977434797676E-4	0.0	0.004585347873589014	0.027952986075533023	2.645393003993662E-4
30	9.69977434797676E-4	0.0	0.004585347873589014	0.05114426474387746	2.645393003993662E-4
31	9.69977434797676E-4	0.0	0.004585347873589014	0.11392825870532702	2.645393003993662E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	795	0.0	26.761005	1
TAGACCG	35	8.869324E-4	26.42857	5
GAACGGT	45	1.3231969E-4	24.666666	20
GTATCAA	2385	0.0	24.589098	1
GACCGTG	50	2.7020718E-4	22.199999	7
TTAGCGA	75	9.265592E-6	19.733334	27
TAGCGAG	75	9.265592E-6	19.733334	28
CGGCGTA	80	1.6166388E-5	18.5	12
ACAACGC	50	0.0070348266	18.499998	3
TTTATAC	100	2.87484E-7	18.499998	3
TATTAGA	635	0.0	18.35433	2
TTTAGGA	400	0.0	17.574999	2
TAGGACC	405	0.0	17.358025	4
GTCGCCC	555	0.0	17.333334	37
GTGACAC	630	0.0	17.325396	24
TCGGCGT	75	2.0672356E-4	17.266666	11
ATCCGTA	65	0.001579916	17.076923	12
TTAACGG	605	0.0	16.818182	35
GACACAT	645	0.0	16.635658	26
ATTAGAG	660	0.0	16.537878	3
>>END_MODULE