FastQCFastQC Report
Fri 10 Feb 2017
ERR1633326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217141
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82050.6741207468978532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57200.469953768708802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32520.2671835062659133No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.1138734131871328No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13340.10960110619887095No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12890.10590391745902898No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA12710.10442504196309221No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12430.10212456896941274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.025.8765811
GTATCAA44500.023.6966291
GGTGCGT450.00382593520.5555578
TTCGCCG3450.019.8405824
CGCCGTC2950.019.44067626
AGCTTCG3600.019.0138921
GCTTCGC3700.019.00000222
GCCGGCA3750.018.74666815
AGTTGCG500.007035115318.49999810
GCCGCTC3800.018.01315927
TCGCCGT3300.017.37878825
GCCGTCC3100.017.30645227
GCGGTAA1502.5102054E-1017.26666623
CGTCCGC2800.017.17857229
CGAGTCG3150.017.03174621
CCGGCAG4350.017.01149616
GGCGAGT3300.016.81818219
CGCTCTC4000.016.6529
ACGGGTA2350.016.53191416
CCGTCCG3050.016.37704828
Produced by FastQC (version 0.11.5)