##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633326.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1217141
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86145894354064	33.0	33.0	33.0	33.0	33.0
2	31.96133562175623	33.0	33.0	33.0	33.0	33.0
3	31.681710664582	33.0	33.0	33.0	27.0	33.0
4	31.964723068239422	33.0	33.0	33.0	33.0	33.0
5	32.06692815376361	33.0	33.0	33.0	33.0	33.0
6	35.20693083217146	37.0	37.0	37.0	33.0	37.0
7	35.363801728805456	37.0	37.0	37.0	33.0	37.0
8	35.5061319929244	37.0	37.0	37.0	33.0	37.0
9	35.618060684834376	37.0	37.0	37.0	33.0	37.0
10	35.619035921064196	37.0	37.0	37.0	33.0	37.0
11	35.644405208599494	37.0	37.0	37.0	33.0	37.0
12	35.6206347497948	37.0	37.0	37.0	33.0	37.0
13	35.63218887540556	37.0	37.0	37.0	33.0	37.0
14	35.61212382131569	37.0	37.0	37.0	33.0	37.0
15	35.64194452409376	37.0	37.0	37.0	33.0	37.0
16	35.62705306944717	37.0	37.0	37.0	33.0	37.0
17	35.61840986377092	37.0	37.0	37.0	33.0	37.0
18	35.602548924077	37.0	37.0	37.0	33.0	37.0
19	35.59082472778421	37.0	37.0	37.0	33.0	37.0
20	35.596721333025506	37.0	37.0	37.0	33.0	37.0
21	35.59600490000748	37.0	37.0	37.0	33.0	37.0
22	35.501382337789956	37.0	37.0	37.0	33.0	37.0
23	35.54834320756593	37.0	37.0	37.0	33.0	37.0
24	35.53615809507691	37.0	37.0	37.0	33.0	37.0
25	35.56113958859327	37.0	37.0	37.0	33.0	37.0
26	35.44999716548863	37.0	37.0	37.0	33.0	37.0
27	35.45786396152952	37.0	37.0	37.0	33.0	37.0
28	35.487452152215724	37.0	37.0	37.0	33.0	37.0
29	35.49101377736844	37.0	37.0	37.0	33.0	37.0
30	35.484817289040464	37.0	37.0	37.0	33.0	37.0
31	35.47856000249766	37.0	37.0	37.0	33.0	37.0
32	35.465119489032084	37.0	37.0	37.0	33.0	37.0
33	35.46099014000843	37.0	37.0	37.0	33.0	37.0
34	35.4359593506422	37.0	37.0	37.0	33.0	37.0
35	35.367595044452536	37.0	37.0	37.0	33.0	37.0
36	35.3987261952395	37.0	37.0	37.0	33.0	37.0
37	35.40002103289594	37.0	37.0	37.0	33.0	37.0
38	35.375636840760436	37.0	37.0	37.0	33.0	37.0
39	35.27021602263008	37.0	37.0	37.0	33.0	37.0
40	35.04790981488587	37.0	37.0	37.0	27.0	37.0
41	35.21356851835572	37.0	37.0	37.0	33.0	37.0
42	35.247732185506855	37.0	37.0	37.0	33.0	37.0
43	34.90172625850251	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	32.0
16	75.0
17	46.0
18	31.0
19	45.0
20	90.0
21	204.0
22	535.0
23	1232.0
24	2645.0
25	4842.0
26	7895.0
27	11845.0
28	16862.0
29	22862.0
30	30406.0
31	38787.0
32	49646.0
33	64880.0
34	92892.0
35	188000.0
36	683282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11196566379737	19.854232171950496	14.080866555312818	23.95293560893931
2	16.089836756793176	21.815056760063133	38.93928476651431	23.15582171662938
3	18.01985143874046	27.585300306209387	30.112780688515134	24.28206756653502
4	12.29192016372795	17.914686959029396	39.8811641379265	29.91222873931615
5	14.10723983499036	36.52847122888802	34.82702497081275	14.537263965308867
6	28.857708350963446	40.21423976351138	17.085859403306603	13.842192482218577
7	26.453714072568417	32.553089576310384	23.02946002147656	17.963736329644632
8	25.023559308247773	34.470123017793334	20.567296640241352	19.93902103371754
9	26.778409403676317	15.17901377079566	19.97500700411867	38.06756982140935
10	15.584554295681437	27.709443688118302	32.89898212286005	23.80701989334021
11	33.901002431106996	24.01940284650669	23.61690223236256	18.462692490023752
12	23.809320366333893	25.89732824709709	29.47226327927496	20.82108810729406
13	28.778013393682407	22.341618596366402	25.653560269516845	23.226807740434346
14	22.657687153748004	21.0932833582962	28.032742303480042	28.216287184475753
15	24.4804012024901	28.19081766204573	24.94879393595319	22.379987199510985
16	22.514564869641234	28.226392833697982	25.31990952568355	23.939132770977235
17	22.723743592566514	26.86024051445149	26.023607782500136	24.39240811048186
18	23.921550584525537	25.99764530157147	27.790864000144598	22.289940113758387
19	25.893384579107924	25.453254799567183	27.631145446583428	21.02221517474146
20	25.312022189705218	25.272667669563347	27.426649829395277	21.988660311336155
21	23.501796422928813	26.30311525123219	27.816908640823044	22.37817968501595
22	23.173403903081073	26.272798303565487	27.7602184134788	22.79357937987464
23	22.984354318850485	26.045133636941	28.229021945690764	22.741490098517755
24	23.014589106767417	26.42594407714472	27.39510048548196	23.164366330605905
25	23.23592747265929	26.436707004365147	28.01704979127316	22.310315731702406
26	23.3747774497778	26.442293867349797	27.61824636586887	22.564682317003534
27	23.290974505008048	26.21167144973343	27.5871899804542	22.910164064804324
28	22.75176006723954	26.275509575308035	27.81764807857101	23.155082278881412
29	23.811127880828924	26.339429860632418	27.268656630579365	22.58078562795929
30	23.810141963831637	25.888783633120564	27.620957637611422	22.680116765436377
31	23.240281939397324	26.036506863214697	27.736556405543812	22.986654791844167
32	22.1318647551927	26.176589236579822	28.053035761674284	23.638510246553192
33	22.218625450954328	26.252669164870788	28.43064197163681	23.09806341253807
34	23.1761973345734	25.826506542791673	27.894796083609048	23.10250003902588
35	22.85018744746911	26.446648334087836	27.57305850349302	23.130105714950034
36	23.328521510654888	26.34222329212474	27.662119672248327	22.667135524972046
37	23.380610792011773	25.892973780359053	27.658668962757808	23.067746464871366
38	23.316936985936714	26.144711253667406	27.356978361586705	23.18137339880918
39	23.00374401979721	25.995180509078242	27.640922456806567	23.36015301431798
40	22.934647670237055	26.612939667630947	27.78519497741018	22.66721768472182
41	22.881901110881977	25.819851603059956	27.756932023487828	23.541315262570237
42	22.386313500243602	26.7356863337937	27.7436221440244	23.134378021938296
43	23.24077489789597	26.029276805234563	27.49081659396898	23.239131702900487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	69.0
2	109.0
3	388.5
4	668.0
5	668.0
6	893.0
7	1118.0
8	1060.0
9	1002.0
10	1418.0
11	1834.0
12	1834.0
13	2910.5
14	3987.0
15	7115.0
16	10243.0
17	11499.5
18	12756.0
19	12756.0
20	11303.0
21	9850.0
22	9983.5
23	10117.0
24	12906.5
25	15696.0
26	15696.0
27	19781.0
28	23866.0
29	28318.0
30	32770.0
31	38154.5
32	43539.0
33	43539.0
34	51429.0
35	59319.0
36	64269.0
37	69219.0
38	73138.5
39	77058.0
40	77058.0
41	81360.0
42	85662.0
43	87630.0
44	89598.0
45	91348.5
46	93099.0
47	93099.0
48	92850.5
49	92602.0
50	93452.0
51	94302.0
52	90942.0
53	87582.0
54	87582.0
55	82402.5
56	77223.0
57	67910.0
58	58597.0
59	54883.0
60	51169.0
61	51169.0
62	44575.5
63	37982.0
64	31814.5
65	25647.0
66	22205.0
67	18763.0
68	18763.0
69	15787.5
70	12812.0
71	10765.0
72	8718.0
73	6772.0
74	4826.0
75	4826.0
76	3532.0
77	2238.0
78	1744.5
79	1251.0
80	998.0
81	745.0
82	745.0
83	610.5
84	476.0
85	410.5
86	345.0
87	262.5
88	180.0
89	180.0
90	148.0
91	116.0
92	70.5
93	25.0
94	14.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1217141.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.27193214212672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.39111323721261	46.34340703150662
2	8.61771923546308	9.35400547133905
3	2.13763835967212	3.4804129200159717
4	0.9632283158908215	2.0910504718960663
5	0.5462623100553634	1.4823355511563034
6	0.34265275773789117	1.115785632975805
7	0.2681506816584785	1.018713891918378
8	0.19975812702221016	0.8673007611670178
9	0.16249133193349458	0.7936846686340567
>10	1.1143135067407282	11.959202956756206
>50	0.14135432816493843	5.353211795220145
>100	0.10532360033351175	10.939809173307422
>500	0.00814912353982151	2.863564531136161
>1k	0.0015375704792116058	1.1755167720630348
>5k	3.075140958423211E-4	1.1619983709077704
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8205	0.6741207468978532	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5720	0.469953768708802	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3252	0.2671835062659133	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.1138734131871328	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1334	0.10960110619887095	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1289	0.10590391745902898	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1271	0.10442504196309221	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1243	0.10212456896941274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.1079874887133043E-4	0.0	0.0
2	1.6431949954853216E-4	0.0	4.1079874887133043E-4	1.6431949954853216E-4	0.0
3	1.6431949954853216E-4	0.0	9.037572475169269E-4	3.286389990970643E-4	0.0
4	1.6431949954853216E-4	0.0	0.0014788754959367896	3.286389990970643E-4	0.0
5	1.6431949954853216E-4	0.0	0.0016431949954853217	3.286389990970643E-4	0.0
6	1.6431949954853216E-4	0.0	0.0023004729936794504	3.286389990970643E-4	0.0
7	1.6431949954853216E-4	0.0	0.006079821483295691	5.751182484198626E-4	0.0
8	1.6431949954853216E-4	0.0	0.007394377479683948	5.751182484198626E-4	0.0
9	1.6431949954853216E-4	0.0	0.009284051724492069	0.0013145559963882573	0.0
10	6.572779981941286E-4	0.0	0.010352128471557528	0.0013145559963882573	0.0
11	6.572779981941286E-4	0.0	0.016267630455304685	0.0013967157461625235	0.0
12	6.572779981941286E-4	0.0	0.01700706820327308	0.0013967157461625235	0.0
13	6.572779981941286E-4	0.0	0.018157304700112804	0.0013967157461625235	0.0
14	6.572779981941286E-4	0.0	0.019143221697403998	0.0015610352457110556	0.0
15	6.572779981941286E-4	0.0	0.020622097193340787	0.0015610352457110556	0.0
16	6.572779981941286E-4	0.0	0.022593931187923175	0.0016431949954853217	0.0
17	6.572779981941286E-4	0.0	0.0234976884354401	0.0017253547452595879	0.0
18	7.394377479683948E-4	8.215974977426608E-5	0.023826327434537165	0.0017253547452595879	0.0
19	7.394377479683948E-4	8.215974977426608E-5	0.02440144568295703	0.0017253547452595879	0.0
20	7.394377479683948E-4	8.215974977426608E-5	0.02497656393137689	0.001971833994582386	0.0
21	7.394377479683948E-4	8.215974977426608E-5	0.02563384192957102	0.0024647924932279827	0.0
22	7.394377479683948E-4	8.215974977426608E-5	0.026291119927765148	0.0032042302411963773	0.0
23	7.394377479683948E-4	8.215974977426608E-5	0.02645543942731368	0.004107987488713305	0.0
24	7.394377479683948E-4	8.215974977426608E-5	0.026537599177087947	0.004354466738036102	0.0
25	8.215974977426609E-4	8.215974977426608E-5	0.026537599177087947	0.004847425236681699	0.0
26	8.215974977426609E-4	8.215974977426608E-5	0.026619758926862212	0.005586862984650094	0.0
27	8.215974977426609E-4	8.215974977426608E-5	0.026619758926862212	0.007476537229458214	0.0
28	8.215974977426609E-4	1.6431949954853216E-4	0.026619758926862212	0.016760588953950283	0.0
29	8.215974977426609E-4	1.6431949954853216E-4	0.026619758926862212	0.04025827738939038	0.0
30	9.037572475169269E-4	1.6431949954853216E-4	0.026619758926862212	0.07230057980135415	0.0
31	9.037572475169269E-4	1.6431949954853216E-4	0.026619758926862212	0.14780538984390468	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1580	0.0	25.876581	1
GTATCAA	4450	0.0	23.696629	1
GGTGCGT	45	0.003825935	20.555557	8
TTCGCCG	345	0.0	19.84058	24
CGCCGTC	295	0.0	19.440676	26
AGCTTCG	360	0.0	19.01389	21
GCTTCGC	370	0.0	19.000002	22
GCCGGCA	375	0.0	18.746668	15
AGTTGCG	50	0.0070351153	18.499998	10
GCCGCTC	380	0.0	18.013159	27
TCGCCGT	330	0.0	17.378788	25
GCCGTCC	310	0.0	17.306452	27
GCGGTAA	150	2.5102054E-10	17.266666	23
CGTCCGC	280	0.0	17.178572	29
CGAGTCG	315	0.0	17.031746	21
CCGGCAG	435	0.0	17.011496	16
GGCGAGT	330	0.0	16.818182	19
CGCTCTC	400	0.0	16.65	29
ACGGGTA	235	0.0	16.531914	16
CCGTCCG	305	0.0	16.377048	28
>>END_MODULE