FastQCFastQC Report
Fri 10 Feb 2017
ERR1633325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143421
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52660.460547777240404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36280.3172934553414709No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20720.18121059522258207No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC17620.15409897141997567No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16800.1469275096399314No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14770.129173768891773No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14710.1286490277859161No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13630.11920368788049197No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12730.11133257129263849No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11740.10267434304599968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT358.869369E-426.428576
GGTATCA12650.026.0316221
TAGACCG400.001931181423.1255
GTATCAA32050.021.9344791
GCCGCTC2450.021.14285727
ATTAGAC1101.7553248E-920.1818183
CCGCTCT2600.019.92307728
GCCGGCA2750.019.5090915
TTAACGG4200.019.3809535
AACGGCC4350.018.71264537
CCGGCAG3000.018.516
GTCTATA609.2367735E-418.51
TGCGGTA1409.458745E-1118.536
GCATTAG801.616659E-518.51
CGCCGTT902.1521973E-618.525
TCGGGTA500.00703486218.49999825
AATACGC500.00703486218.4999985
CGCCGCT3200.017.92187526
GTCCGGC3100.017.90322715
CGCTCTC3000.017.88333329
Produced by FastQC (version 0.11.5)