##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143421
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.822906873321376	33.0	33.0	33.0	27.0	33.0
2	31.926682298121165	33.0	33.0	33.0	33.0	33.0
3	31.649823643260007	33.0	33.0	33.0	27.0	33.0
4	31.94360170051101	33.0	33.0	33.0	33.0	33.0
5	32.03675461619124	33.0	33.0	33.0	33.0	33.0
6	35.17923844323307	37.0	37.0	37.0	33.0	37.0
7	35.335312190348084	37.0	37.0	37.0	33.0	37.0
8	35.4773823464848	37.0	37.0	37.0	33.0	37.0
9	35.59519634500328	37.0	37.0	37.0	33.0	37.0
10	35.58951164968983	37.0	37.0	37.0	33.0	37.0
11	35.61805406757441	37.0	37.0	37.0	33.0	37.0
12	35.59858529797861	37.0	37.0	37.0	33.0	37.0
13	35.606451167155406	37.0	37.0	37.0	33.0	37.0
14	35.58266727653244	37.0	37.0	37.0	33.0	37.0
15	35.606714412276844	37.0	37.0	37.0	33.0	37.0
16	35.589466172127324	37.0	37.0	37.0	33.0	37.0
17	35.58628973929987	37.0	37.0	37.0	33.0	37.0
18	35.57502267318861	37.0	37.0	37.0	33.0	37.0
19	35.55508338573456	37.0	37.0	37.0	33.0	37.0
20	35.56696177523414	37.0	37.0	37.0	33.0	37.0
21	35.572764537296415	37.0	37.0	37.0	33.0	37.0
22	35.47846681143691	37.0	37.0	37.0	33.0	37.0
23	35.52942442022667	37.0	37.0	37.0	33.0	37.0
24	35.52354731984107	37.0	37.0	37.0	33.0	37.0
25	35.538979955764326	37.0	37.0	37.0	33.0	37.0
26	35.43026934086395	37.0	37.0	37.0	33.0	37.0
27	35.4428709985211	37.0	37.0	37.0	33.0	37.0
28	35.4662639570202	37.0	37.0	37.0	33.0	37.0
29	35.469459630354876	37.0	37.0	37.0	33.0	37.0
30	35.46511914684093	37.0	37.0	37.0	33.0	37.0
31	35.458517903729245	37.0	37.0	37.0	33.0	37.0
32	35.45172600468244	37.0	37.0	37.0	33.0	37.0
33	35.44738202289445	37.0	37.0	37.0	33.0	37.0
34	35.42901258591542	37.0	37.0	37.0	33.0	37.0
35	35.36509911922205	37.0	37.0	37.0	33.0	37.0
36	35.39534782026917	37.0	37.0	37.0	33.0	37.0
37	35.39390828050211	37.0	37.0	37.0	33.0	37.0
38	35.385012169620815	37.0	37.0	37.0	33.0	37.0
39	35.278877158981686	37.0	37.0	37.0	33.0	37.0
40	35.080657080812756	37.0	37.0	37.0	33.0	37.0
41	35.24080369347773	37.0	37.0	37.0	33.0	37.0
42	35.284302107447736	37.0	37.0	37.0	33.0	37.0
43	34.95302517620369	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	23.0
16	38.0
17	53.0
18	42.0
19	34.0
20	59.0
21	187.0
22	519.0
23	1258.0
24	2549.0
25	4666.0
26	7641.0
27	11386.0
28	16284.0
29	21697.0
30	28742.0
31	36623.0
32	46717.0
33	61003.0
34	88203.0
35	177849.0
36	637844.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92270388597026	19.723793773247124	13.42943675164266	24.924065589139957
2	16.380580730981855	21.042118344861606	38.92240915638247	23.654891767774075
3	17.804903005979426	27.147043827251732	29.68119354113664	25.366859625632205
4	12.475369964343843	17.609611857749684	39.44968651091767	30.4653316669888
5	14.02020777998655	36.80158052021084	35.07859309912972	14.099618600672892
6	29.709267190299986	39.61428030445479	16.98385808901533	13.692594416229891
7	27.180977085430474	31.923237372761214	23.162247326225422	17.733538215582886
8	24.84447985475166	35.35215812898311	20.20157055012983	19.601791466135396
9	26.641280858056653	15.110969625361086	19.83993647134345	38.407813045238804
10	15.775466779077874	27.42778031888517	33.140199454094336	23.65655344794262
11	34.343168439271274	23.53577553674456	23.483213969307894	18.637842054676273
12	23.781879115391444	25.699370573043524	29.145782699460653	21.37296761210438
13	28.092802213707813	21.96094002121703	25.73426585658301	24.21199190849215
14	22.379333596286934	20.81105734458262	27.563425894749177	29.24618316438127
15	24.84334291568897	27.7096537495813	24.58377098199176	22.863232352737967
16	23.153501641127807	27.753906916175232	24.84159377866945	24.25099766402751
17	22.76781692832299	26.940295831544113	25.84830959025591	24.443577649876993
18	23.59463399745151	26.434620319200015	27.403992055419657	22.566753627928822
19	25.678730756213152	25.462449963749133	26.942044968563632	21.91677431147408
20	24.77206558214341	25.61375031593787	27.240272830392303	22.37391127152641
21	23.800507424649364	26.452111689395245	27.125966726166478	22.621414159788912
22	23.518371623400306	26.27816001280368	26.901902273965582	23.30156608983043
23	23.159273793292236	26.33570662074599	27.59071243225374	22.914307153708037
24	23.65323008760553	26.672590410706114	26.176097867714514	23.49808163397384
25	23.55781466319055	26.188604197404107	27.482615764447218	22.77096537495813
26	23.74357301466389	26.391066807413893	27.2875869867704	22.577773191151813
27	23.700806614536553	26.31567900187245	26.4999505868792	23.4835637967118
28	22.825800820520175	26.100272777918192	27.630330385745932	23.443596015815697
29	24.53129687140607	26.01377795230278	26.622215264543858	22.832709911747294
30	23.943761746548297	25.826532834362848	27.218408617648265	23.01129680144059
31	23.443683472666674	26.08689187971884	27.267909195300767	23.201515452313714
32	22.464953853392583	25.820410854794517	27.54252370736588	24.172111584447023
33	22.610919337671774	26.12353630027785	27.899347659348567	23.366196702701806
34	23.54250971426972	25.893174954806675	27.501856271661968	23.062459059261638
35	23.53979855188946	26.450012724971817	26.994519079149327	23.015669643989398
36	24.33346947449802	26.249299251981554	26.480535165962493	22.936696107557932
37	23.50831408553805	25.775020749137894	26.894818269036513	23.821846896287543
38	23.767186364427452	26.049635261203	26.705386729822173	23.47779164454737
39	22.856935459467685	26.22122560281821	27.2285536123615	23.693285325352605
40	23.28521165869789	26.533883845057943	27.426118638716623	22.754785857527544
41	23.163121894735188	25.534776779506412	27.480429343172812	23.821671982585592
42	22.627798509910175	26.821791798471427	27.062647966059743	23.487761725558652
43	23.723020654684497	25.35172959041333	27.144682492275372	23.7805672626268
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	50.5
2	76.0
3	276.5
4	477.0
5	477.0
6	598.5
7	720.0
8	687.5
9	655.0
10	895.0
11	1135.0
12	1135.0
13	1844.0
14	2553.0
15	4562.0
16	6571.0
17	7449.5
18	8328.0
19	8328.0
20	7323.0
21	6318.0
22	6403.0
23	6488.0
24	8665.5
25	10843.0
26	10843.0
27	14481.0
28	18119.0
29	22392.0
30	26665.0
31	32576.5
32	38488.0
33	38488.0
34	48102.0
35	57716.0
36	63090.0
37	68464.0
38	73793.5
39	79123.0
40	79123.0
41	83632.0
42	88141.0
43	90665.5
44	93190.0
45	91411.5
46	89633.0
47	89633.0
48	87513.5
49	85394.0
50	86455.5
51	87517.0
52	84237.5
53	80958.0
54	80958.0
55	77957.5
56	74957.0
57	65113.0
58	55269.0
59	50713.0
60	46157.0
61	46157.0
62	41333.5
63	36510.0
64	29299.0
65	22088.0
66	18847.5
67	15607.0
68	15607.0
69	13089.0
70	10571.0
71	9147.0
72	7723.0
73	7684.5
74	7646.0
75	7646.0
76	6348.5
77	5051.0
78	3665.5
79	2280.0
80	1539.5
81	799.0
82	799.0
83	642.0
84	485.0
85	424.5
86	364.0
87	275.0
88	186.0
89	186.0
90	143.0
91	100.0
92	64.5
93	29.0
94	15.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1143421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.91610580410897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.90409659549645	42.380818688630896
2	8.652184807676909	8.637667445934097
3	2.243664502110262	3.35984984028778
4	0.9902033905770302	1.9770838884652193
5	0.5882731280130099	1.4682151849805773
6	0.379515805053485	1.136637064762881
7	0.2813526046808555	0.9830818468457859
8	0.21846776903806792	0.8724048219273473
9	0.16791799170801527	0.7543631016459705
>10	1.2495801906546131	12.702301604115657
>50	0.17226672435897183	6.043632467547162
>100	0.13947323874024195	13.662603714860456
>500	0.009796970603623993	3.3837974769814783
>1k	0.003028154550211053	2.1693220916886276
>5k	1.78126738247709E-4	0.46822076132602314
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5266	0.460547777240404	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3628	0.3172934553414709	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2072	0.18121059522258207	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1762	0.15409897141997567	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1680	0.1469275096399314	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1477	0.129173768891773	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1471	0.1286490277859161	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1363	0.11920368788049197	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1273	0.11133257129263849	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1174	0.10267434304599968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.623705529284489E-4	0.0	0.0
2	8.745685097614964E-5	0.0	3.4982740390459855E-4	8.745685097614964E-5	0.0
3	1.7491370195229928E-4	0.0	4.372842548807482E-4	8.745685097614964E-5	0.0
4	1.7491370195229928E-4	0.0	5.247411058568978E-4	1.7491370195229928E-4	0.0
5	1.7491370195229928E-4	0.0	7.871116587853468E-4	1.7491370195229928E-4	0.0
6	1.7491370195229928E-4	0.0	0.0011369390626899453	1.7491370195229928E-4	8.745685097614964E-5
7	1.7491370195229928E-4	0.0	0.0033233603370936863	3.4982740390459855E-4	8.745685097614964E-5
8	1.7491370195229928E-4	0.0	0.004285385697831333	3.4982740390459855E-4	8.745685097614964E-5
9	1.7491370195229928E-4	0.0	0.005072497356616679	0.001224395913666095	8.745685097614964E-5
10	2.623705529284489E-4	0.0	0.005422324760521278	0.001224395913666095	8.745685097614964E-5
11	2.623705529284489E-4	0.0	0.010057537862257208	0.0013118527646422446	8.745685097614964E-5
12	2.623705529284489E-4	0.0	0.010757192670066406	0.0013118527646422446	8.745685097614964E-5
13	2.623705529284489E-4	0.0	0.011719218030804052	0.0013118527646422446	8.745685097614964E-5
14	2.623705529284489E-4	0.0	0.012331415987637099	0.0013118527646422446	8.745685097614964E-5
15	2.623705529284489E-4	0.0	0.013031070795446296	0.0014867664665945439	8.745685097614964E-5
16	2.623705529284489E-4	0.0	0.014080553007160093	0.0014867664665945439	1.7491370195229928E-4
17	2.623705529284489E-4	0.0	0.014168009858136241	0.0015742233175706935	1.7491370195229928E-4
18	2.623705529284489E-4	0.0	0.014255466709112392	0.0017491370195229928	2.623705529284489E-4
19	2.623705529284489E-4	0.0	0.01460529411301699	0.0017491370195229928	2.623705529284489E-4
20	2.623705529284489E-4	0.0	0.014955121516921589	0.0020115075724514418	2.623705529284489E-4
21	2.623705529284489E-4	0.0	0.015130035218873888	0.002186421274403741	3.4982740390459855E-4
22	2.623705529284489E-4	0.0	0.015392405771802337	0.0027986192312367884	3.4982740390459855E-4
23	3.4982740390459855E-4	0.0	0.015392405771802337	0.003235903486117537	3.4982740390459855E-4
24	3.4982740390459855E-4	0.0	0.015567319473754636	0.003760644591974435	3.4982740390459855E-4
25	3.4982740390459855E-4	0.0	0.015742233175706936	0.004285385697831333	3.4982740390459855E-4
26	3.4982740390459855E-4	0.0	0.016004603728635384	0.00489758365466438	3.4982740390459855E-4
27	3.4982740390459855E-4	0.0	0.016004603728635384	0.006209436419306625	3.4982740390459855E-4
28	3.4982740390459855E-4	0.0	0.016004603728635384	0.01705408594034918	4.372842548807482E-4
29	3.4982740390459855E-4	0.0	0.016092060579611534	0.03874338498243429	4.372842548807482E-4
30	3.4982740390459855E-4	0.0	0.016092060579611534	0.07206444520434731	4.372842548807482E-4
31	3.4982740390459855E-4	0.0	0.016179517430587684	0.15007595627507278	4.372842548807482E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	35	8.869369E-4	26.42857	6
GGTATCA	1265	0.0	26.031622	1
TAGACCG	40	0.0019311814	23.125	5
GTATCAA	3205	0.0	21.934479	1
GCCGCTC	245	0.0	21.142857	27
ATTAGAC	110	1.7553248E-9	20.181818	3
CCGCTCT	260	0.0	19.923077	28
GCCGGCA	275	0.0	19.50909	15
TTAACGG	420	0.0	19.38095	35
AACGGCC	435	0.0	18.712645	37
CCGGCAG	300	0.0	18.5	16
GTCTATA	60	9.2367735E-4	18.5	1
TGCGGTA	140	9.458745E-11	18.5	36
GCATTAG	80	1.616659E-5	18.5	1
CGCCGTT	90	2.1521973E-6	18.5	25
TCGGGTA	50	0.007034862	18.499998	25
AATACGC	50	0.007034862	18.499998	5
CGCCGCT	320	0.0	17.921875	26
GTCCGGC	310	0.0	17.903227	15
CGCTCTC	300	0.0	17.883333	29
>>END_MODULE