FastQCFastQC Report
Fri 10 Feb 2017
ERR1633324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159709
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40780.351639937260123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.24704473277348027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16300.14055250067042682No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15620.13468896076515746No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13000.11209708642426679No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12960.1117521723121921No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12770.11011383027983744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.026.117651
GTATCAA25900.022.0714281
TGCGGTA1900.021.42105336
CTTGCGG2000.020.34999834
CCTTGCG2100.020.26190433
CCTAATA1254.129106E-1019.242
AACGGCC3250.018.78461637
CACTAGT801.6166905E-518.57
GCACTAG701.2192093E-418.56
TTGCGGT2250.018.08888835
TTAACGG3400.017.95588335
AGCTTCG3100.017.90322721
GCCGGCA2950.017.55932215
TAACGGC3550.017.19718436
TTTAACG3800.016.55263134
CCGCTCT3700.016.528
CACCGTC803.3825974E-416.18757
TTCGCCG3450.016.08695624
GTATTAG5900.015.9915261
CTGCTCG7350.015.8571429
Produced by FastQC (version 0.11.5)