FastQCFastQC Report
Fri 10 Feb 2017
ERR1633323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862320
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34010.3944011503850079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.2617357825401243No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC14620.16954262918638094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12980.15052416736246405No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11350.13162167176918083No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9780.11341497355969943No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9640.11179144633082845No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8770.10170238426570183No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8630.10007885703683086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.026.9698771
ATGTTCG551.9019437E-523.54545423
GTATCAA21100.021.919431
CGCATCT450.00382504520.55555515
TATAGAC450.00382504520.5555553
CGCCGAC450.00382504520.55555528
TAGACTG555.141661E-420.1818185
ACGCACT555.141661E-420.18181833
TTAGACT609.234266E-418.54
CGCTATC801.6159076E-518.512
CTAGTAC500.007033492418.4999983
AACGCAC500.007033492418.49999832
GTCAACC2900.017.86206815
GCGTCAA852.7218379E-517.41176426
ATCGCTA752.0665115E-417.26666610
GCCGGCA2050.017.14634115
CGACCGT650.00157950117.07692331
CGCACTC650.00157950117.07692334
CGCTCTC1901.8189894E-1216.55263129
CGAACTA2350.016.53191429
Produced by FastQC (version 0.11.5)