##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	862320
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82613646906021	33.0	33.0	33.0	27.0	33.0
2	31.93742114296317	33.0	33.0	33.0	33.0	33.0
3	31.65265562668151	33.0	33.0	33.0	27.0	33.0
4	31.944768763336118	33.0	33.0	33.0	33.0	33.0
5	32.039827442248814	33.0	33.0	33.0	33.0	33.0
6	35.2118042026162	37.0	37.0	37.0	33.0	37.0
7	35.35528690045459	37.0	37.0	37.0	33.0	37.0
8	35.49027623156137	37.0	37.0	37.0	33.0	37.0
9	35.60543185824288	37.0	37.0	37.0	33.0	37.0
10	35.61006934780592	37.0	37.0	37.0	33.0	37.0
11	35.640102282215416	37.0	37.0	37.0	33.0	37.0
12	35.61562181092866	37.0	37.0	37.0	33.0	37.0
13	35.62263080990815	37.0	37.0	37.0	33.0	37.0
14	35.606545134056965	37.0	37.0	37.0	33.0	37.0
15	35.61853720196679	37.0	37.0	37.0	33.0	37.0
16	35.601460014843674	37.0	37.0	37.0	33.0	37.0
17	35.59229172464978	37.0	37.0	37.0	33.0	37.0
18	35.58578833843585	37.0	37.0	37.0	33.0	37.0
19	35.56297894053252	37.0	37.0	37.0	33.0	37.0
20	35.57146071064106	37.0	37.0	37.0	33.0	37.0
21	35.56933388997124	37.0	37.0	37.0	33.0	37.0
22	35.477843491975136	37.0	37.0	37.0	33.0	37.0
23	35.533307820762595	37.0	37.0	37.0	33.0	37.0
24	35.52823661749699	37.0	37.0	37.0	33.0	37.0
25	35.5457173671027	37.0	37.0	37.0	33.0	37.0
26	35.43549494387234	37.0	37.0	37.0	33.0	37.0
27	35.45155046850357	37.0	37.0	37.0	33.0	37.0
28	35.473057565636886	37.0	37.0	37.0	33.0	37.0
29	35.48092239539846	37.0	37.0	37.0	33.0	37.0
30	35.474304202616196	37.0	37.0	37.0	33.0	37.0
31	35.464958484089436	37.0	37.0	37.0	33.0	37.0
32	35.46252899155766	37.0	37.0	37.0	33.0	37.0
33	35.45356712125429	37.0	37.0	37.0	33.0	37.0
34	35.43200204100566	37.0	37.0	37.0	33.0	37.0
35	35.380917756749234	37.0	37.0	37.0	33.0	37.0
36	35.410368540680956	37.0	37.0	37.0	33.0	37.0
37	35.414270804341776	37.0	37.0	37.0	33.0	37.0
38	35.40205724093144	37.0	37.0	37.0	33.0	37.0
39	35.30724672975229	37.0	37.0	37.0	33.0	37.0
40	35.11068048984136	37.0	37.0	37.0	33.0	37.0
41	35.273066842935336	37.0	37.0	37.0	33.0	37.0
42	35.319367520178126	37.0	37.0	37.0	33.0	37.0
43	34.9911610539011	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	22.0
16	45.0
17	32.0
18	30.0
19	24.0
20	65.0
21	141.0
22	383.0
23	922.0
24	1887.0
25	3448.0
26	5570.0
27	8411.0
28	11982.0
29	16216.0
30	21056.0
31	27323.0
32	35012.0
33	46264.0
34	66819.0
35	135592.0
36	481073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50918452546619	19.94456814175712	13.519111234808424	25.027136097968274
2	16.298473884404864	20.929005473606086	38.864342703404766	23.908177938584284
3	17.978940532516933	26.870535300120608	29.65070971333148	25.499814454030982
4	12.576421745987568	17.5127562853697	39.22696910659616	30.683852862046574
5	14.010112255311252	37.04518044345487	35.01008906206513	13.934618239168753
6	30.127562853697004	39.34177567492346	17.068605622042863	13.462055849336673
7	27.143287874570927	31.882015956953335	23.150802486315985	17.823893682159756
8	25.05960664254569	35.4033305501438	20.19922998422859	19.337832823081918
9	26.36921328509138	15.332243250765377	19.798798589850637	38.49974487429261
10	15.573105111791447	27.6389275442991	33.14233695147973	23.645630392429727
11	34.25213377864365	23.47434826978384	23.69584377029409	18.57767418127841
12	23.74489748585212	25.49529177103628	29.380276463493832	21.379534279617776
13	27.929422952036365	21.926199090824753	25.877284534743485	24.267093422395398
14	22.243366731607754	20.65834029130717	27.47808238241024	29.620210594674827
15	24.369723536506168	28.014426199090824	24.39303274886353	23.222817515539475
16	23.134567214027275	27.966880044531035	24.6966323406624	24.201920400779294
17	22.793858428425644	27.171119769922996	25.82405603488264	24.210965766768716
18	23.575818721588274	26.658664996752947	27.291376751090084	22.4741395305687
19	25.41840616012617	25.843190462937194	26.811740421189352	21.926662955747286
20	24.5204796363299	25.976319695704607	27.076027460803413	22.427173207162074
21	23.81111420354393	26.500371091938028	26.975484738844045	22.713029965673996
22	23.429469338528623	26.45746358660358	26.767209388626036	23.345857686241768
23	23.266420818257725	26.326305779756936	27.37637999814454	23.030893403840803
24	23.654559792188515	26.807681603117175	26.0590036181464	23.478754986547916
25	23.4221634659987	26.472771129047224	27.19512477966416	22.909940625289916
26	23.705468967436683	26.52298450691159	27.217506262176457	22.554040263475276
27	23.593445588644588	26.539799610353466	26.20384544020781	23.66290936079414
28	22.947513684015213	26.244781519621483	27.539660450876706	23.268044345486594
29	24.44266629557473	26.06897671398089	26.533769366360517	22.954587624083867
30	23.923717413489193	26.044275906855923	27.063387141664347	22.968619537990538
31	23.44790796919937	26.141107709435012	27.110701363762875	23.300282957602747
32	22.552068837554504	26.123132943686798	27.28836162909361	24.03643658966509
33	22.787596251971426	26.252435290843305	27.579900732906577	23.380067724278693
34	23.55633639484182	26.096692643102326	27.23803228499861	23.108938677057242
35	23.51540031542815	26.615409592726596	26.848501716300216	23.020688375545042
36	24.282516931069672	26.3265377122182	26.325146117450597	23.065799239261526
37	23.354091288616754	25.988148251229244	26.80165135912422	23.85610910102978
38	23.88069394192411	26.237591613322202	26.613670099267097	23.268044345486594
39	22.86865664718434	26.254058818072178	27.09852490954634	23.778759625197143
40	23.318141757120326	26.62700621578996	27.268415437424622	22.78643658966509
41	22.96224139530569	25.888649225345578	27.306104462380553	23.84300491696818
42	22.737846739029592	26.91251507560998	26.90196214862232	23.447676036738102
43	23.59553298079599	25.548172372205215	27.089479543556916	23.76681510344188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	45.5
2	52.0
3	179.0
4	306.0
5	306.0
6	377.5
7	449.0
8	435.5
9	422.0
10	588.0
11	754.0
12	754.0
13	1203.5
14	1653.0
15	2970.5
16	4288.0
17	4985.5
18	5683.0
19	5683.0
20	5325.0
21	4967.0
22	5617.0
23	6267.0
24	8143.0
25	10019.0
26	10019.0
27	13097.5
28	16176.0
29	19340.0
30	22504.0
31	26844.0
32	31184.0
33	31184.0
34	37744.5
35	44305.0
36	48398.0
37	52491.0
38	55834.0
39	59177.0
40	59177.0
41	62073.0
42	64969.0
43	66093.5
44	67218.0
45	65879.0
46	64540.0
47	64540.0
48	62289.5
49	60039.0
50	61189.5
51	62340.0
52	60364.0
53	58388.0
54	58388.0
55	57323.5
56	56259.0
57	49559.0
58	42859.0
59	39289.5
60	35720.0
61	35720.0
62	32317.5
63	28915.0
64	23647.5
65	18380.0
66	15611.0
67	12842.0
68	12842.0
69	10914.5
70	8987.0
71	7712.5
72	6438.0
73	6290.5
74	6143.0
75	6143.0
76	5175.0
77	4207.0
78	3056.5
79	1906.0
80	1253.0
81	600.0
82	600.0
83	483.0
84	366.0
85	309.5
86	253.0
87	196.0
88	139.0
89	139.0
90	101.0
91	63.0
92	37.5
93	12.0
94	6.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	862320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.19854764181307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.72383321934409	49.60477306077303
2	7.846040578051679	8.97564251606575
3	1.9274292749298654	3.3073846562490337
4	0.8965690539531637	2.0512979098686124
5	0.49858976079982614	1.4259305093414518
6	0.32628888803603634	1.1197950304394062
7	0.22538231948418247	0.9024078937046841
8	0.1683795010094946	0.770485032831703
9	0.1482848912978643	0.7633512377515768
>10	0.9933687856887554	11.306750737340947
>50	0.13648382247153532	5.501220269445825
>100	0.10236137717825602	10.901676854770766
>500	0.0059608030853817925	2.2461503380391172
>1k	0.0010277246698934125	1.1231339533780522
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3401	0.3944011503850079	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2257	0.2617357825401243	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1462	0.16954262918638094	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1298	0.15052416736246405	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1135	0.13162167176918083	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	978	0.11341497355969943	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	964	0.11179144633082845	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	877	0.10170238426570183	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	863	0.10007885703683086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.6386492253455794E-4	0.0	0.0
2	1.1596623063363949E-4	0.0	4.6386492253455794E-4	0.0	0.0
3	1.1596623063363949E-4	0.0	4.6386492253455794E-4	0.0	0.0
4	1.1596623063363949E-4	0.0	4.6386492253455794E-4	0.0	0.0
5	2.3193246126727897E-4	0.0	4.6386492253455794E-4	1.1596623063363949E-4	0.0
6	2.3193246126727897E-4	0.0	0.0012756285369700344	1.1596623063363949E-4	0.0
7	2.3193246126727897E-4	0.0	0.0018554596901382318	1.1596623063363949E-4	0.0
8	3.4789869190091847E-4	0.0	0.002435290843306429	1.1596623063363949E-4	0.0
9	3.4789869190091847E-4	0.0	0.003131088227108266	9.277298450691159E-4	0.0
10	4.6386492253455794E-4	0.0	0.0034789869190091846	0.0010436960757027553	0.0
11	5.798311531681975E-4	0.0	0.005798311531681974	0.0010436960757027553	0.0
12	5.798311531681975E-4	0.0	0.006146210223582893	0.0010436960757027553	0.0
13	5.798311531681975E-4	0.0	0.006494108915483811	0.0010436960757027553	0.0
14	5.798311531681975E-4	0.0	0.007305872529919288	0.0010436960757027553	0.0
15	5.798311531681975E-4	0.0	0.007885703683087485	0.001159662306336395	0.0
16	5.798311531681975E-4	0.0	0.00892939975879024	0.001159662306336395	3.4789869190091847E-4
17	5.798311531681975E-4	0.0	0.00927729845069116	0.0012756285369700344	3.4789869190091847E-4
18	5.798311531681975E-4	0.0	0.009741163373225717	0.0012756285369700344	3.4789869190091847E-4
19	5.798311531681975E-4	0.0	0.009973095834492995	0.0013915947676036739	3.4789869190091847E-4
20	5.798311531681975E-4	0.0	0.010089062065126634	0.0016235272288709528	3.4789869190091847E-4
21	5.798311531681975E-4	0.0	0.010436960757027554	0.0017394934595045923	4.6386492253455794E-4
22	5.798311531681975E-4	0.0	0.010668893218294832	0.0017394934595045923	4.6386492253455794E-4
23	5.798311531681975E-4	0.0	0.010784859448928472	0.00231932461267279	4.6386492253455794E-4
24	5.798311531681975E-4	0.0	0.011016791910195752	0.002667223304573708	4.6386492253455794E-4
25	5.798311531681975E-4	0.0	0.011016791910195752	0.0027831895352073478	4.6386492253455794E-4
26	5.798311531681975E-4	0.0	0.011132758140829391	0.0037109193802764636	5.798311531681975E-4
27	5.798311531681975E-4	0.0	0.011132758140829391	0.0049865479172464975	5.798311531681975E-4
28	5.798311531681975E-4	0.0	0.011132758140829391	0.012756285369700342	5.798311531681975E-4
29	5.798311531681975E-4	0.0	0.011132758140829391	0.02899155765840987	5.798311531681975E-4
30	5.798311531681975E-4	0.0	0.011132758140829391	0.05172093886260321	5.798311531681975E-4
31	5.798311531681975E-4	0.0	0.011132758140829391	0.11596623063363948	5.798311531681975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	830	0.0	26.969877	1
ATGTTCG	55	1.9019437E-5	23.545454	23
GTATCAA	2110	0.0	21.91943	1
CGCATCT	45	0.003825045	20.555555	15
TATAGAC	45	0.003825045	20.555555	3
CGCCGAC	45	0.003825045	20.555555	28
TAGACTG	55	5.141661E-4	20.181818	5
ACGCACT	55	5.141661E-4	20.181818	33
TTAGACT	60	9.234266E-4	18.5	4
CGCTATC	80	1.6159076E-5	18.5	12
CTAGTAC	50	0.0070334924	18.499998	3
AACGCAC	50	0.0070334924	18.499998	32
GTCAACC	290	0.0	17.862068	15
GCGTCAA	85	2.7218379E-5	17.411764	26
ATCGCTA	75	2.0665115E-4	17.266666	10
GCCGGCA	205	0.0	17.146341	15
CGACCGT	65	0.001579501	17.076923	31
CGCACTC	65	0.001579501	17.076923	34
CGCTCTC	190	1.8189894E-12	16.552631	29
CGAACTA	235	0.0	16.531914	29
>>END_MODULE