FastQCFastQC Report
Fri 10 Feb 2017
ERR1633322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010903
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50000.494607296644683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.34226824927812066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18900.1869615581316902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.023.7323231
GTATCAA25100.022.8486041
CGAGTCG1350.021.92592621
TCGCCGT1500.020.96666725
CCGTCCG1403.6379788E-1219.8214328
TGCGGTA1457.2759576E-1219.1379336
GCGAGTC1650.019.06060620
CGTATGC500.00703430918.536
TTTCGGG1205.171387E-918.522
TTGCGGT1501.2732926E-1118.535
CGTCCGC1501.2732926E-1118.529
GCCGGCA2050.018.0487815
AGTCGCC1750.017.9714323
GAGTCGC1653.6379788E-1217.93939422
GGTAACG1502.5102054E-1017.26666625
TCCGCCG1401.8681021E-917.17857231
GCGGGTA1401.8681021E-917.17857222
CGCCGTC1757.2759576E-1216.91428626
AACGGCC2000.016.65000237
TTAACGG2000.016.65000235
Produced by FastQC (version 0.11.5)