##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633322.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1010903
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87591786749075	33.0	33.0	33.0	33.0	33.0
2	31.985022301843006	33.0	33.0	33.0	33.0	33.0
3	31.700449993718486	33.0	33.0	33.0	27.0	33.0
4	31.98222381375859	33.0	33.0	33.0	33.0	33.0
5	32.08804603409031	33.0	33.0	33.0	33.0	33.0
6	35.25520945135191	37.0	37.0	37.0	33.0	37.0
7	35.40030349103722	37.0	37.0	37.0	33.0	37.0
8	35.53931188254462	37.0	37.0	37.0	33.0	37.0
9	35.65983778859099	37.0	37.0	37.0	33.0	37.0
10	35.656049096698695	37.0	37.0	37.0	33.0	37.0
11	35.68263324967875	37.0	37.0	37.0	33.0	37.0
12	35.661564957270876	37.0	37.0	37.0	33.0	37.0
13	35.66399051145362	37.0	37.0	37.0	33.0	37.0
14	35.646334020178	37.0	37.0	37.0	33.0	37.0
15	35.657517091155135	37.0	37.0	37.0	33.0	37.0
16	35.642768890783785	37.0	37.0	37.0	33.0	37.0
17	35.6413216698338	37.0	37.0	37.0	33.0	37.0
18	35.62064312797568	37.0	37.0	37.0	33.0	37.0
19	35.61685938215635	37.0	37.0	37.0	33.0	37.0
20	35.61480676187527	37.0	37.0	37.0	33.0	37.0
21	35.6157020010822	37.0	37.0	37.0	33.0	37.0
22	35.527299849738306	37.0	37.0	37.0	33.0	37.0
23	35.5653173449876	37.0	37.0	37.0	33.0	37.0
24	35.5557506506559	37.0	37.0	37.0	33.0	37.0
25	35.58197967559697	37.0	37.0	37.0	33.0	37.0
26	35.481068905720925	37.0	37.0	37.0	33.0	37.0
27	35.49656198468102	37.0	37.0	37.0	33.0	37.0
28	35.51340435234637	37.0	37.0	37.0	33.0	37.0
29	35.518732262145825	37.0	37.0	37.0	33.0	37.0
30	35.52377824578619	37.0	37.0	37.0	33.0	37.0
31	35.51680329368891	37.0	37.0	37.0	33.0	37.0
32	35.509927263050955	37.0	37.0	37.0	33.0	37.0
33	35.50404934993763	37.0	37.0	37.0	33.0	37.0
34	35.478060704142734	37.0	37.0	37.0	33.0	37.0
35	35.41892050968293	37.0	37.0	37.0	33.0	37.0
36	35.44757508880674	37.0	37.0	37.0	33.0	37.0
37	35.439176656909716	37.0	37.0	37.0	33.0	37.0
38	35.431911864936595	37.0	37.0	37.0	33.0	37.0
39	35.33450588236458	37.0	37.0	37.0	33.0	37.0
40	35.12825365044915	37.0	37.0	37.0	33.0	37.0
41	35.28740739714888	37.0	37.0	37.0	33.0	37.0
42	35.33722424406694	37.0	37.0	37.0	33.0	37.0
43	34.999972301991384	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	28.0
16	45.0
17	29.0
18	35.0
19	33.0
20	68.0
21	152.0
22	425.0
23	989.0
24	2088.0
25	3828.0
26	6395.0
27	9636.0
28	13496.0
29	18437.0
30	24085.0
31	31484.0
32	39764.0
33	53073.0
34	76475.0
35	155488.0
36	574846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.483010733967554	20.177207902241857	13.6065478092359	24.73323355455469
2	16.032200913440757	21.594950257344177	38.59331706405066	23.779531765164414
3	18.011916078990765	26.74598848752056	30.20002908290904	25.042066350579628
4	12.640678680348163	17.644521779043092	39.3325571296158	30.382242410992944
5	14.173268849731379	36.587981240534454	35.04352049603177	14.195229413702403
6	29.662984480212245	39.5643301088235	17.167027894862315	13.60565751610194
7	26.76517925063038	32.221686947214515	23.135750907851694	17.87738289430341
8	24.871327911777886	35.233350776483995	20.62443181986798	19.27088949187014
9	26.399862301328614	15.053373073380927	20.21786462202605	38.32890000326441
10	15.017464583644525	28.068667320207773	33.625283533632796	23.2885845625149
11	34.12177033800474	23.417578145479833	24.05601724398879	18.404634272526643
12	23.000822037327023	25.609183076912423	30.180244791043254	21.209750094717297
13	28.40964959051462	22.083424423510465	26.012683709515155	23.494242276459758
14	22.24308365886737	20.880935163907914	27.773188921192244	29.10279225603248
15	23.999730933630627	28.488391072140452	24.43666701948654	23.075210974742383
16	22.985192446753054	28.141077828436558	25.072534160052946	23.80119556475745
17	22.535099806806393	27.33417548469042	26.34070726864991	23.79001743985328
18	23.52332518550247	26.66566426254547	27.676542655427873	22.134467896524196
19	25.122588418473384	26.05294474346203	27.25345557387801	21.571011264186573
20	24.52777368352849	25.914355778942195	27.405102171029267	22.15276836650005
21	23.34625577330367	26.53775881563315	27.544383585764407	22.571601825298767
22	22.970650992231697	26.542507045680942	27.472071998994956	23.014769963092405
23	22.886468830342775	26.339223446759974	27.7658687332019	23.00843898969535
24	23.027530831345835	26.71037676216215	26.961439425938988	23.30065298055303
25	23.40204747636519	26.54725527572873	27.662396886743835	22.388300361162248
26	23.527183122416297	26.491760337045196	27.33981400787217	22.64124253266634
27	23.32558118830392	26.62906332259376	27.12574796988435	22.919607519217966
28	22.7816120834541	26.56041182981948	27.491262762104775	23.16671332462165
29	23.601572059831653	26.360491560515698	26.981025874886118	23.05691050476653
30	23.458630551101344	26.190643414847912	27.589096085381087	22.761629948669654
31	23.354367332968643	26.30262250680827	27.378393377010457	22.964616783212634
32	22.47831888915158	26.384925160969946	27.765571968823917	23.37118398105456
33	22.634219109053983	26.34822529955891	28.0089187587731	23.00863683261401
34	23.12694689797142	26.16937530109219	27.510156760836598	23.193521040099792
35	22.85481396335751	26.78901932232865	27.399265804928863	22.956900909384974
36	23.73353328657646	26.2907519316888	27.27927407476286	22.696440706971885
37	23.421831768230977	26.063034732313582	27.22358129316067	23.291552206294767
38	23.247334313974733	26.379583402166183	27.25612645327989	23.116955830579194
39	22.937314460437847	26.319142390516205	27.509761074999282	23.23378207404667
40	23.092818994502935	26.714927149291277	27.553187595644683	22.6390662605611
41	23.0673961794554	25.952242697865174	27.670508446408803	23.30985267627062
42	22.655685065728363	26.614719710991064	27.479491108444627	23.250104114835942
43	23.107459370483618	26.12199192207363	27.391648852560536	23.378899854882217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	52.0
2	74.0
3	281.0
4	488.0
5	488.0
6	619.0
7	750.0
8	714.5
9	679.0
10	909.5
11	1140.0
12	1140.0
13	1835.5
14	2531.0
15	4448.5
16	6366.0
17	7426.5
18	8487.0
19	8487.0
20	7989.5
21	7492.0
22	8302.0
23	9112.0
24	11627.5
25	14143.0
26	14143.0
27	17671.0
28	21199.0
29	24926.0
30	28653.0
31	33370.0
32	38087.0
33	38087.0
34	44483.5
35	50880.0
36	54603.5
37	58327.0
38	61578.5
39	64830.0
40	64830.0
41	68286.0
42	71742.0
43	73987.5
44	76233.0
45	76474.5
46	76716.0
47	76716.0
48	75424.5
49	74133.0
50	75351.5
51	76570.0
52	73702.5
53	70835.0
54	70835.0
55	67278.5
56	63722.0
57	56389.0
58	49056.0
59	45952.5
60	42849.0
61	42849.0
62	37788.0
63	32727.0
64	27233.5
65	21740.0
66	18627.5
67	15515.0
68	15515.0
69	12942.5
70	10370.0
71	8541.0
72	6712.0
73	5347.0
74	3982.0
75	3982.0
76	2995.5
77	2009.0
78	1532.0
79	1055.0
80	853.5
81	652.0
82	652.0
83	536.0
84	420.0
85	355.0
86	290.0
87	236.0
88	182.0
89	182.0
90	138.0
91	94.0
92	60.0
93	26.0
94	15.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1010903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.0239102256467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.69405015519797	49.14279962851626
2	8.75806831421491	10.163547392281737
3	2.356724608203113	4.102391312788495
4	1.1000054552772893	2.5530647113892426
5	0.6444936623100114	1.8698021201437174
6	0.4221055010464247	1.4695327019081617
7	0.29178219370740394	1.1851240669184462
8	0.21430360685485117	0.9947786596142535
9	0.1765956789485473	0.9222094639392873
>10	1.1401617204035601	12.803120670852048
>50	0.12743856627893901	5.107876348708328
>100	0.07115429134156644	7.669846614792372
>500	0.002596871846191337	0.9762097619383715
>1k	5.193743692382674E-4	1.0396965462093173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5000	0.494607296644683	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3460	0.34226824927812066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1890	0.1869615581316902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.956858373157464E-4	0.0	0.0
2	0.0	0.0	3.956858373157464E-4	0.0	0.0
3	0.0	0.0	3.956858373157464E-4	0.0	0.0
4	0.0	0.0	5.935287559736196E-4	1.978429186578732E-4	0.0
5	9.89214593289366E-5	0.0	8.902931339604295E-4	1.978429186578732E-4	0.0
6	9.89214593289366E-5	0.0	0.002077350645907669	1.978429186578732E-4	0.0
7	9.89214593289366E-5	0.0	0.004451465669802147	2.967643779868098E-4	0.0
8	1.978429186578732E-4	0.0	0.00613313047839407	2.967643779868098E-4	0.0
9	1.978429186578732E-4	0.0	0.007715873827657055	8.902931339604295E-4	0.0
10	3.956858373157464E-4	0.0	0.008902931339604294	8.902931339604295E-4	0.0
11	4.94607296644683E-4	0.0	0.013057632631419631	9.89214593289366E-4	0.0
12	4.94607296644683E-4	0.0	0.013849004306051125	9.89214593289366E-4	0.0
13	5.935287559736196E-4	0.0	0.014739297440011553	9.89214593289366E-4	0.0
14	6.924502153025562E-4	0.0	0.015332826195985173	0.0010881360526183027	0.0
15	6.924502153025562E-4	0.0	0.01602527641128773	0.0010881360526183027	0.0
16	6.924502153025562E-4	0.0	0.017212333923234967	0.0011870575119472392	0.0
17	6.924502153025562E-4	0.0	0.017904784138537525	0.0013849004306051124	0.0
18	6.924502153025562E-4	0.0	0.018399391435182207	0.0015827433492629857	0.0
19	6.924502153025562E-4	0.0	0.018696155813169017	0.0016816648085919222	0.0
20	6.924502153025562E-4	0.0	0.019289684569142637	0.0016816648085919222	0.0
21	6.924502153025562E-4	0.0	0.019883213325116257	0.001780586267920859	0.0
22	6.924502153025562E-4	0.0	0.020179977703103067	0.002077350645907669	0.0
23	6.924502153025562E-4	0.0	0.02037782062176094	0.003066565239197035	0.0
24	6.924502153025562E-4	0.0	0.020476742081089877	0.0034622510765127813	0.0
25	6.924502153025562E-4	0.0	0.020872427918405625	0.0036600939951706543	0.0
26	6.924502153025562E-4	0.0	0.020872427918405625	0.004055779832486401	0.0
27	7.913716746314928E-4	0.0	0.020872427918405625	0.005737444641078323	0.0
28	7.913716746314928E-4	0.0	0.020872427918405625	0.01572851203330092	0.0
29	7.913716746314928E-4	0.0	0.02097134937773456	0.030665652391970347	0.0
30	7.913716746314928E-4	0.0	0.02097134937773456	0.055198174305546625	0.0
31	7.913716746314928E-4	0.0	0.02097134937773456	0.11227585633834304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	990	0.0	23.732323	1
GTATCAA	2510	0.0	22.848604	1
CGAGTCG	135	0.0	21.925926	21
TCGCCGT	150	0.0	20.966667	25
CCGTCCG	140	3.6379788E-12	19.82143	28
TGCGGTA	145	7.2759576E-12	19.13793	36
GCGAGTC	165	0.0	19.060606	20
CGTATGC	50	0.007034309	18.5	36
TTTCGGG	120	5.171387E-9	18.5	22
TTGCGGT	150	1.2732926E-11	18.5	35
CGTCCGC	150	1.2732926E-11	18.5	29
GCCGGCA	205	0.0	18.04878	15
AGTCGCC	175	0.0	17.97143	23
GAGTCGC	165	3.6379788E-12	17.939394	22
GGTAACG	150	2.5102054E-10	17.266666	25
TCCGCCG	140	1.8681021E-9	17.178572	31
GCGGGTA	140	1.8681021E-9	17.178572	22
CGCCGTC	175	7.2759576E-12	16.914286	26
AACGGCC	200	0.0	16.650002	37
TTAACGG	200	0.0	16.650002	35
>>END_MODULE