FastQCFastQC Report
Fri 10 Feb 2017
ERR1633321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048716
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110631.0549090506867447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78920.7525392956720408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42800.4081181177744976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.18870695212049785No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15060.14360417882439097No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13340.12720317035307938No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13150.12539143104520195No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12640.12052834132405724No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11440.10908577727430496No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.102124884144039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.023.1707921
GGTCGGT400.001931074723.12500211
GTATCAA51950.023.0048121
TACGGCT450.003825587220.5555574
TTCGCCG3500.020.08571424
CGCTCTC3250.019.92307729
TCTGTCG656.901395E-519.9230778
GCCGCTC3500.019.0285727
CCGCTCT3550.018.76056328
TAGACCG609.236078E-418.55
GACGTAT801.6164498E-518.528
CCGCTAT609.236078E-418.515
CGTTACG609.236078E-418.516
AACGGTC701.2190678E-418.55
TAACCGT609.236078E-418.57
AAACCCG500.0070344818.55
GCTTCGC4050.017.81481622
CTGTCGC752.067049E-417.2666669
CGGTCCG752.067049E-417.26666621
CTGCTCG5050.017.2178219
Produced by FastQC (version 0.11.5)