##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1048716
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.856412985021684	33.0	33.0	33.0	33.0	33.0
2	31.9668346816488	33.0	33.0	33.0	33.0	33.0
3	31.673945090949314	33.0	33.0	33.0	27.0	33.0
4	31.95390267717857	33.0	33.0	33.0	33.0	33.0
5	32.06444166008719	33.0	33.0	33.0	33.0	33.0
6	35.193720702268294	37.0	37.0	37.0	33.0	37.0
7	35.354612688277854	37.0	37.0	37.0	33.0	37.0
8	35.499310585516	37.0	37.0	37.0	33.0	37.0
9	35.617300584715025	37.0	37.0	37.0	33.0	37.0
10	35.60247102170654	37.0	37.0	37.0	33.0	37.0
11	35.63342220391412	37.0	37.0	37.0	33.0	37.0
12	35.615670972884935	37.0	37.0	37.0	33.0	37.0
13	35.62601791142693	37.0	37.0	37.0	33.0	37.0
14	35.61022526594426	37.0	37.0	37.0	33.0	37.0
15	35.640120871618244	37.0	37.0	37.0	33.0	37.0
16	35.62153910114845	37.0	37.0	37.0	33.0	37.0
17	35.61720046227959	37.0	37.0	37.0	33.0	37.0
18	35.603135643968436	37.0	37.0	37.0	33.0	37.0
19	35.58599563656891	37.0	37.0	37.0	33.0	37.0
20	35.59624435976947	37.0	37.0	37.0	33.0	37.0
21	35.59138699133035	37.0	37.0	37.0	33.0	37.0
22	35.485095106778196	37.0	37.0	37.0	33.0	37.0
23	35.53191998596379	37.0	37.0	37.0	33.0	37.0
24	35.52974017751231	37.0	37.0	37.0	33.0	37.0
25	35.55492812162683	37.0	37.0	37.0	33.0	37.0
26	35.44825863246103	37.0	37.0	37.0	33.0	37.0
27	35.45878007010477	37.0	37.0	37.0	33.0	37.0
28	35.477393307625704	37.0	37.0	37.0	33.0	37.0
29	35.47673154600483	37.0	37.0	37.0	33.0	37.0
30	35.47237478974289	37.0	37.0	37.0	33.0	37.0
31	35.462592351027354	37.0	37.0	37.0	33.0	37.0
32	35.44063693125689	37.0	37.0	37.0	33.0	37.0
33	35.43060370967927	37.0	37.0	37.0	33.0	37.0
34	35.396572570648296	37.0	37.0	37.0	33.0	37.0
35	35.325642023197894	37.0	37.0	37.0	33.0	37.0
36	35.33262866209727	37.0	37.0	37.0	33.0	37.0
37	35.314637137223045	37.0	37.0	37.0	33.0	37.0
38	35.29425602355643	37.0	37.0	37.0	33.0	37.0
39	35.19003428955027	37.0	37.0	37.0	33.0	37.0
40	34.94926271745639	37.0	37.0	37.0	27.0	37.0
41	35.09532609400448	37.0	37.0	37.0	27.0	37.0
42	35.11813398479665	37.0	37.0	37.0	33.0	37.0
43	34.76456256984732	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	32.0
16	56.0
17	43.0
18	34.0
19	37.0
20	70.0
21	177.0
22	480.0
23	1108.0
24	2287.0
25	4241.0
26	6896.0
27	10396.0
28	15008.0
29	20168.0
30	26710.0
31	34391.0
32	43417.0
33	57084.0
34	81352.0
35	161727.0
36	582999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.0839235789289	19.48868902543682	14.259055835898375	23.168331559735904
2	15.860633384062034	22.501611494437007	39.4642591511906	22.173495970310363
3	18.273584078053542	27.892870901178203	30.686858978026464	23.14668604274179
4	12.344047387471917	17.71137276440905	39.5445478089397	30.400032039179337
5	14.103627674222574	36.791848317370956	33.88114608721523	15.223377921191247
6	28.350192044366633	40.19229228885609	16.946342002982696	14.511173663794583
7	25.783338863905957	33.32274896158731	22.547000331834358	18.346911842672373
8	25.133782644681684	34.09578951784849	20.508793610472235	20.261634226997586
9	27.300622856903107	14.669557821183238	19.76111740452134	38.26870191739232
10	15.461669317527338	27.75079239756045	32.657077797992976	24.13046048691924
11	33.52947795208617	24.27549498624985	23.319754823994295	18.875272237669684
12	23.764203082626757	26.229789571247125	29.626514709416085	20.379492636710033
13	29.470133000736137	22.62662150668055	25.917979700891376	21.985265791691937
14	23.064013517482334	21.572189229495876	28.702432307698178	26.661364945323612
15	24.57567158315502	27.787408602519655	25.803363350993024	21.833556463332304
16	21.92585981333364	28.60831721838896	25.54781275388189	23.91801021439551
17	22.272664858741546	26.705132752813913	26.397995262778483	24.624207125666054
18	24.13351183733251	24.99504155557844	28.45670324472975	22.414743362359303
19	25.797642068968145	25.010870435847266	28.949877755274066	20.24160973991052
20	25.615705300577083	24.507206908257338	28.49827789411051	21.378809897055064
21	23.25691607642107	26.377589356889757	28.191140404075078	22.17435416261409
22	22.704430942218863	26.19803645600906	28.495989381300564	22.60154322047151
23	22.496366985914204	25.83654678673731	29.130956331361396	22.53612989598709
24	22.862529035506277	26.017911426925878	28.846894678826295	22.272664858741546
25	22.72960458312832	25.947158239218243	29.27770721529947	22.045529962353964
26	22.9267027488853	26.06101175151328	28.566647214307782	22.445638285293633
27	22.876736885868052	25.91473764107728	28.856430148867755	22.35209532418691
28	22.316718730333093	26.171146430492144	28.68011930780116	22.832015531373603
29	23.062106423474038	26.039938362721653	28.269331258415054	22.62862395538926
30	22.861480133801713	26.287383810297545	28.724649952894776	22.126486103005963
31	23.05390591923838	25.931234004249003	28.687270910332252	22.32758916618036
32	21.74897684406455	25.990544627906885	28.857002277070247	23.403476250958317
33	21.944644689315314	25.952784166542703	29.54660747046865	22.55596367367333
34	22.13163525682835	25.823101773978845	28.694994641065836	23.350268328126965
35	22.20267450863723	26.687015359735145	28.745341922884748	22.364968208742884
36	22.71329892935742	26.210432567062963	28.71683086746078	22.359437636118834
37	23.243471063662614	26.091811319747194	28.143653763268606	22.521063853321586
38	23.027492667223537	26.3602348014143	28.059074144000856	22.553198387361306
39	22.682690070524338	25.88403342754378	28.314338677010742	23.118937824921144
40	22.613271848622507	26.41325201484482	28.552057945144348	22.421418191388327
41	22.632628852806672	25.851517474702394	28.689178004340548	22.826675668150386
42	22.212496042779932	26.315608801620265	28.625099645661933	22.846795509937866
43	22.346183332761203	26.11784315296038	28.39901365097891	23.136959863299502
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	119.0
2	152.0
3	632.5
4	1113.0
5	1113.0
6	1360.0
7	1607.0
8	1540.5
9	1474.0
10	2036.0
11	2598.0
12	2598.0
13	4055.0
14	5512.0
15	9889.5
16	14267.0
17	15779.5
18	17292.0
19	17292.0
20	14965.0
21	12638.0
22	12140.0
23	11642.0
24	14117.0
25	16592.0
26	16592.0
27	19694.0
28	22796.0
29	28031.0
30	33266.0
31	35732.5
32	38199.0
33	38199.0
34	42195.5
35	46192.0
36	49259.0
37	52326.0
38	55196.5
39	58067.0
40	58067.0
41	61460.5
42	64854.0
43	65348.0
44	65842.0
45	69592.5
46	73343.0
47	73343.0
48	75021.0
49	76699.0
50	80125.5
51	83552.0
52	84115.5
53	84679.0
54	84679.0
55	76869.0
56	69059.0
57	62914.5
58	56770.0
59	50538.5
60	44307.0
61	44307.0
62	37995.0
63	31683.0
64	25975.5
65	20268.0
66	17614.5
67	14961.0
68	14961.0
69	12376.0
70	9791.0
71	8522.0
72	7253.0
73	5806.0
74	4359.0
75	4359.0
76	3217.5
77	2076.0
78	1595.0
79	1114.0
80	908.0
81	702.0
82	702.0
83	627.0
84	552.0
85	509.0
86	466.0
87	408.0
88	350.0
89	350.0
90	254.5
91	159.0
92	104.5
93	50.0
94	29.0
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1048716.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.893474069710294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.413738840741	47.43550332320933
2	7.991317200752855	8.773423270847132
3	1.9359666989195163	3.188158133608833
4	0.8578736218038757	1.8836665365431802
5	0.5163134744768404	1.417112016151823
6	0.34694959012838433	1.1427160997525447
7	0.23932728244678175	0.9196254181216652
8	0.19090422737351512	0.8383517004100903
9	0.15598315044246586	0.7706211321712697
>10	1.0859492513135458	12.143095684561207
>50	0.15927083677658754	6.122840683741461
>100	0.099182912974053	10.214084670996709
>500	0.005461226033366076	1.974233692236608
>1k	0.0014093486537718907	1.343523325311937
>5k	1.7616858172148634E-4	0.7631962918996477
>10k+	1.7616858172148634E-4	1.0698480204366196
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11063	1.0549090506867447	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7892	0.7525392956720408	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4280	0.4081181177744976	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1979	0.18870695212049785	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1506	0.14360417882439097	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1334	0.12720317035307938	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1315	0.12539143104520195	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1264	0.12052834132405724	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1144	0.10908577727430496	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1071	0.102124884144039	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.628376033168179E-4	0.0	0.0
2	0.0	0.0	8.581923037314201E-4	1.9070940082920447E-4	0.0
3	0.0	0.0	9.535470041460224E-4	1.9070940082920447E-4	0.0
4	0.0	0.0	0.001144256404975227	3.8141880165840894E-4	0.0
5	0.0	0.0	0.0016210299070482381	3.8141880165840894E-4	0.0
6	0.0	0.0	0.002860641012438067	4.767735020730112E-4	0.0
7	9.535470041460223E-5	0.0	0.006865538429851361	6.674829029022156E-4	0.0
8	9.535470041460223E-5	0.0	0.007914440134411985	6.674829029022156E-4	0.0
9	2.8606410124380673E-4	0.0	0.009916888843118633	0.0013349658058044312	0.0
10	0.001144256404975227	0.0	0.011251854648923063	0.0014303205062190336	0.0
11	0.0013349658058044312	0.0	0.017449910175872208	0.0015256752066336358	0.0
12	0.0013349658058044312	0.0	0.018212747779189027	0.0015256752066336358	0.0
13	0.0013349658058044312	0.0	0.020119841787481073	0.0015256752066336358	0.0
14	0.0014303205062190336	0.0	0.021073388791627096	0.0015256752066336358	0.0
15	0.0014303205062190336	0.0	0.022694418698675334	0.0016210299070482381	0.0
16	0.0014303205062190336	0.0	0.024029384504479764	0.0017163846074628403	0.0
17	0.0015256752066336358	0.0	0.024315448605723572	0.0019070940082920448	0.0
18	0.0015256752066336358	0.0	0.024601512706967376	0.0021931581095358515	0.0
19	0.0015256752066336358	0.0	0.025364350310284195	0.0021931581095358515	0.0
20	0.0015256752066336358	0.0	0.025841123812357206	0.002383867510365056	0.0
21	0.0015256752066336358	0.0	0.02689002551691783	0.003146705113681874	0.0
22	0.0015256752066336358	0.0	0.02736679901899084	0.004195606818242498	0.0
23	0.0015256752066336358	0.0	0.027462153719405445	0.005149153822388521	0.0
24	0.0016210299070482381	0.0	0.027462153719405445	0.0065794743286075544	0.0
25	0.0016210299070482381	0.0	0.027462153719405445	0.007628376033168179	0.0
26	0.0016210299070482381	0.0	0.027462153719405445	0.009916888843118633	0.0
27	0.0016210299070482381	0.0	0.027462153719405445	0.012586820454727496	0.0
28	0.0016210299070482381	0.0	0.02765286312023465	0.026699316116088626	0.0
29	0.0017163846074628403	0.0	0.02765286312023465	0.05463824333756708	0.0
30	0.0017163846074628403	0.0	0.02765286312023465	0.10002708073491774	0.0
31	0.0017163846074628403	0.0	0.02765286312023465	0.1925211401370819	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2020	0.0	23.170792	1
GGTCGGT	40	0.0019310747	23.125002	11
GTATCAA	5195	0.0	23.004812	1
TACGGCT	45	0.0038255872	20.555557	4
TTCGCCG	350	0.0	20.085714	24
CGCTCTC	325	0.0	19.923077	29
TCTGTCG	65	6.901395E-5	19.923077	8
GCCGCTC	350	0.0	19.02857	27
CCGCTCT	355	0.0	18.760563	28
TAGACCG	60	9.236078E-4	18.5	5
GACGTAT	80	1.6164498E-5	18.5	28
CCGCTAT	60	9.236078E-4	18.5	15
CGTTACG	60	9.236078E-4	18.5	16
AACGGTC	70	1.2190678E-4	18.5	5
TAACCGT	60	9.236078E-4	18.5	7
AAACCCG	50	0.00703448	18.5	5
GCTTCGC	405	0.0	17.814816	22
CTGTCGC	75	2.067049E-4	17.266666	9
CGGTCCG	75	2.067049E-4	17.266666	21
CTGCTCG	505	0.0	17.217821	9
>>END_MODULE