FastQCFastQC Report
Fri 10 Feb 2017
ERR1633320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134379
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82290.7254189296522591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53720.4735630684277477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31720.27962435834937No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14490.12773508677434967No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14370.12667723926483127No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.12094723192160645No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12170.10728336825699346No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11750.10358090197367899No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11700.10314013217804631No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11400.10049551340425024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.025.0359291
CGTGATA400.001931171523.12500225
ACGAACG400.001931171523.12500229
GTATCAA41500.021.5759031
ACGCCGG555.143036E-420.18181835
CCGTTAA1950.019.92307716
GCCGTCC3150.018.79365227
GCTTCGC3600.017.9861122
TTCGCCG3500.017.9714324
CCGTCCG3250.017.64615428
CGTCCGC3150.017.6190529
AGCTTCG3700.017.521
CGCCGTC3300.017.37878826
GTATTAG4800.017.343751
TATTGCG650.001579917317.07692111
CGTTAAA2300.016.89130417
GCCGCTC3750.016.77333327
ACTGCTC7950.016.7547198
GCTCGGA3100.016.70967911
CGCTCTC3600.016.44444529
Produced by FastQC (version 0.11.5)