FastQCFastQC Report
Fri 10 Feb 2017
ERR1633319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917405
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45250.4932390819757904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31210.3401987126732468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17880.1948975643254615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.026.456991
GTATCAA25050.023.485031
GGACCGT450.003825228720.5555556
TATCGCC450.003825228720.55555533
GGTCGTC555.142007E-420.18181811
TTCGCCG1551.8189894E-1219.09677524
GCCGGCA1501.2732926E-1118.515
AAACGCC609.2348794E-418.511
TGCTCGC2150.018.06976710
CTGCTCG4200.018.0595259
GTCGCCC1800.017.47222137
CGAACTA2150.017.20930329
CGGAGTA650.001579605517.07692322
TTTCGTC1005.8751903E-616.6517
TCCTATA1352.217348E-816.4444452
CATTCGG1708.54925E-1116.3235337
CCGCTCT1708.54925E-1116.3235328
AGGACCG803.381635E-416.18755
TGGGGCG1606.2937033E-1016.187537
GCTTCGC1951.8189894E-1216.12820622
Produced by FastQC (version 0.11.5)