FastQCFastQC Report
Fri 10 Feb 2017
ERR1633318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078104
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97150.9011190015063483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69900.6483604550210369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38840.36026208974273355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.16464088807758806No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA14960.1387621231346883No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14260.13226924304148766No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13880.12874453670517874No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12690.11770664054673761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG358.869036E-426.42857230
GGTATCA18800.024.6010651
GTATCAA46700.022.1049251
GCCGTCC2950.020.69491827
TTGTCGC555.14281E-420.18181837
TCGCGGA555.14281E-420.18181832
ACGGTCA555.14281E-420.18181811
GCCGGCA4200.019.82142815
AGCTTCG4550.019.51648321
TTCGCCG4000.019.42500124
GCTTCGC4450.019.12359422
CGTCCGC3000.019.11666729
CGCCGTC3200.019.07812526
CGAGTCG3400.018.521
GACGTGT500.007034606318.535
CCGCTCT4000.018.528
CCGTCCG3100.018.528
GGTATAC801.6165188E-518.53
TTAACGG1205.171387E-918.535
CCGGCAG4550.018.29670316
Produced by FastQC (version 0.11.5)