##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1078104
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.765365864517708	33.0	33.0	33.0	27.0	33.0
2	31.883543702648353	33.0	33.0	33.0	33.0	33.0
3	31.60512622158901	33.0	33.0	33.0	27.0	33.0
4	31.895753099886466	33.0	33.0	33.0	33.0	33.0
5	31.99791949570728	33.0	33.0	33.0	33.0	33.0
6	35.13032972700222	37.0	37.0	37.0	33.0	37.0
7	35.295791500634444	37.0	37.0	37.0	33.0	37.0
8	35.44232931145789	37.0	37.0	37.0	33.0	37.0
9	35.55878839147244	37.0	37.0	37.0	33.0	37.0
10	35.55770686315977	37.0	37.0	37.0	33.0	37.0
11	35.5825996378828	37.0	37.0	37.0	33.0	37.0
12	35.55989589130548	37.0	37.0	37.0	33.0	37.0
13	35.56382593887046	37.0	37.0	37.0	33.0	37.0
14	35.55011668633082	37.0	37.0	37.0	33.0	37.0
15	35.57942832973442	37.0	37.0	37.0	33.0	37.0
16	35.564196960590074	37.0	37.0	37.0	33.0	37.0
17	35.55325367497013	37.0	37.0	37.0	33.0	37.0
18	35.53649369634098	37.0	37.0	37.0	33.0	37.0
19	35.5258778373886	37.0	37.0	37.0	33.0	37.0
20	35.535245208254494	37.0	37.0	37.0	33.0	37.0
21	35.52051286332302	37.0	37.0	37.0	33.0	37.0
22	35.42843083784125	37.0	37.0	37.0	33.0	37.0
23	35.469624451815406	37.0	37.0	37.0	33.0	37.0
24	35.465714810445	37.0	37.0	37.0	33.0	37.0
25	35.48636216914138	37.0	37.0	37.0	33.0	37.0
26	35.36931409214696	37.0	37.0	37.0	33.0	37.0
27	35.38572902057687	37.0	37.0	37.0	33.0	37.0
28	35.40195843814697	37.0	37.0	37.0	33.0	37.0
29	35.400530004526466	37.0	37.0	37.0	33.0	37.0
30	35.406145418252784	37.0	37.0	37.0	33.0	37.0
31	35.388374405437695	37.0	37.0	37.0	33.0	37.0
32	35.36966285256339	37.0	37.0	37.0	33.0	37.0
33	35.36281471917366	37.0	37.0	37.0	33.0	37.0
34	35.33198837959974	37.0	37.0	37.0	33.0	37.0
35	35.24888229706967	37.0	37.0	37.0	33.0	37.0
36	35.2683349658289	37.0	37.0	37.0	33.0	37.0
37	35.257635626989604	37.0	37.0	37.0	33.0	37.0
38	35.23379840905886	37.0	37.0	37.0	33.0	37.0
39	35.12344727410343	37.0	37.0	37.0	33.0	37.0
40	34.89131104234842	37.0	37.0	37.0	27.0	37.0
41	35.04676264998553	37.0	37.0	37.0	27.0	37.0
42	35.07445478358303	37.0	37.0	37.0	33.0	37.0
43	34.707556042830745	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	26.0
16	32.0
17	47.0
18	38.0
19	42.0
20	77.0
21	235.0
22	513.0
23	1238.0
24	2495.0
25	4677.0
26	7706.0
27	11539.0
28	16165.0
29	21769.0
30	28449.0
31	36598.0
32	46671.0
33	60217.0
34	86515.0
35	169512.0
36	583538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51315271996023	19.731955358666696	14.19130250884887	23.56358941252421
2	15.950780258676343	22.205557163316342	38.90209107841173	22.941571499595586
3	18.08665954304965	27.660503995903916	30.596306107759546	23.656530353286882
4	12.087145581502341	17.848741865348796	40.273665620385415	29.79044693276344
5	14.034545832313022	36.595263536727444	34.61623368431988	14.753956946639658
6	28.430652330387417	40.626043498586405	16.951147570178758	13.992156600847414
7	26.1292973590674	32.98076994427254	22.875529633504748	18.014403063155317
8	25.29876523971713	34.29687673916431	20.574267417614628	19.83009060350393
9	27.01882193183589	14.78280388533945	20.028308957206356	38.17006522561831
10	15.429030965472718	27.87690241386731	32.93903000081625	23.755036619843725
11	33.61345473163999	24.128469980632666	23.76626002686197	18.491815260865373
12	23.578059259589057	26.130039402506622	29.716799121420568	20.575102216483753
13	29.102479909173883	22.45506926975505	25.7960270994264	22.646423721644666
14	22.51424723403308	21.44774530100992	28.898974495966996	27.13903296899001
15	24.396996950201466	28.218613417629467	25.332249949912068	22.052139682257
16	21.873678235123883	28.430652330387417	25.91744395716925	23.77822547731944
17	22.512113859145312	26.819026735825112	26.45449789630685	24.21436150872272
18	23.854655951559405	25.52295511379236	28.493262245571856	22.12912668907638
19	25.523326135511976	25.541969976922452	28.523129494000578	20.411574393564997
20	25.34644153068721	25.06938106156734	28.034864910991892	21.549312496753558
21	23.238203364424955	26.422590028420263	28.290035098654677	22.049171508500105
22	22.896956137812307	26.257114341473546	28.490572338104673	22.35535718260947
23	22.69029703998872	26.03329548911793	28.834787738474212	22.441619732419134
24	22.62110148928118	26.172150367682523	28.599652723670445	22.607095419365848
25	22.814589316058562	26.275108894874705	28.97753834509472	21.93276344397201
26	23.013735224060017	26.343655157572925	28.363497399137742	22.27911221922931
27	23.018744017274773	26.026988119884535	28.594365664165984	22.35990219867471
28	22.408227777654105	26.27297551998694	28.418408613640246	22.900388088718714
29	23.05009535258194	26.385024079309606	28.189209946350257	22.375670621758196
30	23.426589642557673	26.094977850003335	28.37787449077269	22.100558016666298
31	22.83137804887098	26.129204603637497	28.477215556198658	22.562201791292864
32	21.881098669516113	26.21509613172755	28.76707627464512	23.136728924111218
33	21.9033599726928	26.34124351639545	29.1419009668826	22.613495544029146
34	22.750402558565778	25.950186623924964	28.501331967973403	22.79807884953585
35	22.307680891639396	26.459413934091703	28.340865074241446	22.892040100027454
36	22.716917848370844	26.40264761099115	28.600302011679762	22.280132528958248
37	23.112427001476664	26.14738466789846	28.355705943025907	22.38448238759897
38	22.993236274051483	26.27650022632325	27.997669983600844	22.732593516024426
39	22.651247003999615	25.94230241238322	28.33585628102669	23.070594302590475
40	22.48642060506222	26.457187803774033	28.658459666228865	22.397931924934884
41	22.621843532720405	25.85177311279802	28.329085134643783	23.19729821983779
42	22.01132729309974	26.701412850708277	28.45430496501265	22.83295489117933
43	22.652823846307964	26.44707746191462	28.14236845424931	22.757730237528104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	103.0
2	149.0
3	530.5
4	912.0
5	912.0
6	1153.5
7	1395.0
8	1297.0
9	1199.0
10	1715.0
11	2231.0
12	2231.0
13	3581.5
14	4932.0
15	8746.5
16	12561.0
17	14094.0
18	15627.0
19	15627.0
20	13914.0
21	12201.0
22	12301.0
23	12401.0
24	15091.0
25	17781.0
26	17781.0
27	21287.5
28	24794.0
29	28686.5
30	32579.0
31	36746.0
32	40913.0
33	40913.0
34	46024.5
35	51136.0
36	54592.5
37	58049.0
38	60470.0
39	62891.0
40	62891.0
41	65888.0
42	68885.0
43	70582.5
44	72280.0
45	74377.0
46	76474.0
47	76474.0
48	77926.5
49	79379.0
50	80808.5
51	82238.0
52	80771.0
53	79304.0
54	79304.0
55	73842.0
56	68380.0
57	60568.0
58	52756.0
59	49182.0
60	45608.0
61	45608.0
62	40119.5
63	34631.0
64	28932.0
65	23233.0
66	19927.0
67	16621.0
68	16621.0
69	13783.0
70	10945.0
71	9269.0
72	7593.0
73	5799.0
74	4005.0
75	4005.0
76	2812.5
77	1620.0
78	1248.5
79	877.0
80	707.0
81	537.0
82	537.0
83	450.0
84	363.0
85	325.5
86	288.0
87	224.0
88	160.0
89	160.0
90	118.5
91	77.0
92	57.5
93	38.0
94	21.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1078104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.51448884861502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.28223255106906	49.32710758189615
2	7.590689294554069	8.579678509807547
3	1.751822163370098	2.9701000234960815
4	0.761949074857891	1.7224464997707523
5	0.44751869612698425	1.2645645180907592
6	0.309625518930233	1.0498996762097574
7	0.2409735672111951	0.9532948583877645
8	0.18118292719478288	0.8191568414807187
9	0.13598969863838353	0.6916849476502898
>10	1.0509227444992855	12.021827246907238
>50	0.14543576400570604	5.777941001241446
>100	0.09500281161800526	10.216117506535989
>500	0.0048250112451802525	1.6840528695877068
>1k	0.0014974172829869748	1.3513795556512853
>5k	3.327593962193277E-4	1.5707483632865795
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9715	0.9011190015063483	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6990	0.6483604550210369	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3884	0.36026208974273355	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1775	0.16464088807758806	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1496	0.1387621231346883	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1426	0.13226924304148766	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1388	0.12874453670517874	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1269	0.11770664054673761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.420434392229321E-4	0.0	0.0
2	0.0	0.0	7.420434392229321E-4	0.0	0.0
3	0.0	0.0	9.275542990286651E-4	0.0	0.0
4	0.0	0.0	0.0012058205887372647	0.0	0.0
5	0.0	0.0	0.0012985760186401312	9.275542990286651E-5	0.0
6	0.0	0.0	0.002504396607377396	1.8551085980573302E-4	0.0
7	9.275542990286651E-5	0.0	0.005750836653977724	1.8551085980573302E-4	0.0
8	1.8551085980573302E-4	0.0	0.007142168102520722	1.8551085980573302E-4	0.0
9	1.8551085980573302E-4	0.0	0.00927554299028665	6.492880093200656E-4	0.0
10	6.492880093200656E-4	0.0	0.010481363579023916	7.420434392229321E-4	0.0
11	8.347988691257987E-4	0.0	0.015490156793778707	8.347988691257987E-4	0.0
12	9.275542990286651E-4	0.0	0.017716287111447505	8.347988691257987E-4	0.0
13	9.275542990286651E-4	0.0	0.01920037398989337	8.347988691257987E-4	0.0
14	9.275542990286651E-4	0.0	0.020406194578630632	8.347988691257987E-4	0.0
15	0.0010203097289315317	0.0	0.0216120151673679	9.275542990286651E-4	0.0
16	0.0010203097289315317	0.0	0.023003346615910896	0.0010203097289315317	0.0
17	0.0010203097289315317	0.0	0.02346712376542523	0.0014840868784458641	0.0
18	0.0010203097289315317	0.0	0.024116411774745293	0.0014840868784458641	0.0
19	0.0010203097289315317	0.0	0.02504396607377396	0.0016695977382515973	0.0
20	0.0010203097289315317	0.0	0.025322232363482557	0.0019478640279601968	0.0
21	0.0010203097289315317	0.0	0.026157031232608357	0.0022261303176687964	0.0
22	0.0010203097289315317	0.0	0.02662080838212269	0.002875418326988862	0.0
23	0.0010203097289315317	0.0	0.02689907467183129	0.0033391954765031946	0.0
24	0.0010203097289315317	0.0	0.02689907467183129	0.004637771495143325	0.0
25	0.0010203097289315317	0.0	0.02689907467183129	0.0049160377848519255	0.0
26	0.0010203097289315317	0.0	0.02689907467183129	0.005843592083880591	0.0
27	0.0011130651588343982	0.0	0.02689907467183129	0.008162477831452254	0.0
28	0.0011130651588343982	0.0	0.02689907467183129	0.01947864027960197	0.0
29	0.0011130651588343982	0.0	0.02689907467183129	0.04860384526910205	0.0
30	0.0011130651588343982	0.0	0.02689907467183129	0.0853349955106372	0.0
31	0.0011130651588343982	0.0	0.02689907467183129	0.1692786595727314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGCG	35	8.869036E-4	26.428572	30
GGTATCA	1880	0.0	24.601065	1
GTATCAA	4670	0.0	22.104925	1
GCCGTCC	295	0.0	20.694918	27
TTGTCGC	55	5.14281E-4	20.181818	37
TCGCGGA	55	5.14281E-4	20.181818	32
ACGGTCA	55	5.14281E-4	20.181818	11
GCCGGCA	420	0.0	19.821428	15
AGCTTCG	455	0.0	19.516483	21
TTCGCCG	400	0.0	19.425001	24
GCTTCGC	445	0.0	19.123594	22
CGTCCGC	300	0.0	19.116667	29
CGCCGTC	320	0.0	19.078125	26
CGAGTCG	340	0.0	18.5	21
GACGTGT	50	0.0070346063	18.5	35
CCGCTCT	400	0.0	18.5	28
CCGTCCG	310	0.0	18.5	28
GGTATAC	80	1.6165188E-5	18.5	3
TTAACGG	120	5.171387E-9	18.5	35
CCGGCAG	455	0.0	18.296703	16
>>END_MODULE