FastQCFastQC Report
Fri 10 Feb 2017
ERR1633317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931261
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT307083.2974644057895692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT225572.4221995767029867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127691.3711515890819008No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG60230.6467574611199224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54620.58651656195202No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA38040.4084783964967931No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA37480.4024650447081967No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG37370.40128385060686533No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT34830.37400900499430345No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA33920.3642373083378344No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG32030.3439422460513218No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA30700.3296605355534056No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.3292310104256487No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT28930.3106540486501636No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC28800.30925809198495374No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA25050.26899011125774624No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA24510.2631915220330283No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA23970.25739293280831044No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA22750.2442924164117256No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG22320.23967502128833915No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21800.2340911946274997No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20470.21980948412958345No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC19910.21379613234098713No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC19720.2117558879841419No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18990.20391705440257887No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC18600.19972918440694926No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA18230.19575607697519815No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC17900.19221249467120388No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.19113868185181168No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT17690.18995748775048027No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC17610.1890984374949665No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17030.18287032314249174No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.182011272886978No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16420.17632006494419933No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG16280.17481672699705025No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT16150.17342077033184036No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA16030.17213219494856974No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15950.17127314469305596No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA15920.17095100084723833No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG15900.17073623828335988No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT15570.1671926559793656No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC15050.1616088293185262No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT14870.1596759662436202No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14800.15892429727004567No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.15656190906738282No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14440.15505857112023375No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14260.15312570804532777No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14220.1526961829175709No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA14020.15054855727878652No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA13530.14528687446376473No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13510.14507211189988628No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC13490.14485734933600783No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13240.14217281728752734No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC13230.14206543600558813No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA13000.13959566652098607No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12880.13830709113771542No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC12780.13723327831832322No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT12690.13626684678087025No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC12660.1359447029350526No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12550.13476350883372115No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT12430.1334749334504505No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG12320.1322937393491191No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12280.1318642142213622No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC12240.13143468909360534No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT12100.12993135114645626No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA11900.12778372550767186No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA11770.126387768842462No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11760.12628038756052276No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11750.12617300627858355No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11720.1258508624327659No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA11430.12273680525652853No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG11430.12273680525652853No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11330.12166299243713632No Hit
TATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATGA11180.12005227320804801No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.11854893526089894No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11040.11854893526089894No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG11000.11811941013314205No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10670.1145758278291478No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA10620.11403892141945168No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG10560.11339463372781637No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10390.11156915193484963No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG10310.11071010167933587No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC10270.110280576551579No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10260.11017319526963976No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10190.10942152629606523No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10170.1092067637321868No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.10738128193922006No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC9990.10727390065728083No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9990.10727390065728083No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG9980.10716651937534162No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9950.10684437552952394No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG9690.10405246219910423No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9460.10158269271450217No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT9400.10093840502286683No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9380.1007236424589884No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC9370.10061626117704918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTT200.001841756437.08
TAACAAC603.45608E-1133.9166681
CTAGTCG451.0795884E-732.888899
TGTCAAC551.8550054E-830.2727282
TCTCAAC250.00549581429.62
ACCAACG250.00549581429.62
AGCAACG454.0071845E-628.7777772
TAGCAAC509.088668E-625.9000021
ACTAGTG2500.025.98
GTTATGC659.387804E-825.6153851
CTAGTGG951.0913936E-1125.315799
GGTATCA46600.024.6931341
TCCCTAT601.3369518E-624.6666681
GGCAGCT13800.023.1920318
GTATCAA121800.023.1325951
TAGCAAG400.001930913223.12500212
CGAATAG400.001930913223.12500225
TCGTATT802.7244823E-823.12500214
CCGCTCT13200.023.12528
CGCTCTC13050.023.1072829
Produced by FastQC (version 0.11.5)