FastQCFastQC Report
Fri 10 Feb 2017
ERR1633316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705524
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28560.40480550626201234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.30629716352668374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12730.18043326662168827No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9740.13805341845210087No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8790.1245882493012286No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8210.11636740918806447No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8170.11580045469750143No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8070.11438306847109384No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8040.11395785260317154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG454.0053746E-628.77777715
GCCGGCA905.456968E-1226.72222115
GAATCGG601.3361841E-624.66666822
TTGGTCG451.322505E-424.66666610
AATCGGG701.9207255E-723.78571323
GGTATCA7300.023.315071
CCGCTCT1200.023.12500228
CGCTCTC1200.023.12500229
TCGGCGT400.001930452223.12511
TTCGCCG1155.456968E-1222.5217424
GCGGAAT502.7006684E-422.219
CTTCGCC1350.021.92592623
GCCGCTC1201.0913936E-1121.58333427
GGAATCG705.097316E-621.14285721
GTATCAA16900.020.689351
CGGAATC656.897302E-519.92307720
AGCTTCG1303.274181E-1119.92307721
GCTTCGC1355.638867E-1119.18518622
TCGCCGC1551.8189894E-1219.09677525
CGGCGTA500.007032253318.512
Produced by FastQC (version 0.11.5)