##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633316.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705524
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3077471496363	33.0	33.0	33.0	27.0	33.0
2	31.550073420606527	33.0	33.0	33.0	27.0	33.0
3	31.276404488011746	33.0	33.0	33.0	27.0	33.0
4	31.594413513927236	33.0	33.0	33.0	27.0	33.0
5	31.71559153196773	33.0	33.0	33.0	27.0	33.0
6	34.644695290309045	37.0	37.0	37.0	27.0	37.0
7	34.83752643425312	37.0	37.0	37.0	27.0	37.0
8	34.95851310515305	37.0	37.0	37.0	27.0	37.0
9	35.09365096013743	37.0	37.0	37.0	33.0	37.0
10	35.12752932572102	37.0	37.0	37.0	33.0	37.0
11	35.0893449407816	37.0	37.0	37.0	33.0	37.0
12	35.102805857773795	37.0	37.0	37.0	33.0	37.0
13	35.11023579637263	37.0	37.0	37.0	33.0	37.0
14	35.11861538374315	37.0	37.0	37.0	33.0	37.0
15	35.104639955550766	37.0	37.0	37.0	33.0	37.0
16	35.08545988513502	37.0	37.0	37.0	33.0	37.0
17	35.08213753182032	37.0	37.0	37.0	33.0	37.0
18	35.07822838060789	37.0	37.0	37.0	33.0	37.0
19	35.05531774964424	37.0	37.0	37.0	33.0	37.0
20	35.05566075711103	37.0	37.0	37.0	33.0	37.0
21	35.06532024424399	37.0	37.0	37.0	33.0	37.0
22	34.96548381061452	37.0	37.0	37.0	27.0	37.0
23	35.021997834233844	37.0	37.0	37.0	27.0	37.0
24	34.995889579943416	37.0	37.0	37.0	27.0	37.0
25	35.001284151921126	37.0	37.0	37.0	27.0	37.0
26	34.89425306580641	37.0	37.0	37.0	27.0	37.0
27	34.903777901247864	37.0	37.0	37.0	27.0	37.0
28	34.922643311921355	37.0	37.0	37.0	27.0	37.0
29	34.913651413701025	37.0	37.0	37.0	27.0	37.0
30	34.91797160691911	37.0	37.0	37.0	27.0	37.0
31	34.91455570611347	37.0	37.0	37.0	27.0	37.0
32	34.89107953804548	37.0	37.0	37.0	27.0	37.0
33	34.90386861396636	37.0	37.0	37.0	27.0	37.0
34	34.862651022502426	37.0	37.0	37.0	27.0	37.0
35	34.79590063555598	37.0	37.0	37.0	27.0	37.0
36	34.819286090905486	37.0	37.0	37.0	27.0	37.0
37	34.824383011775645	37.0	37.0	37.0	27.0	37.0
38	34.80081613098917	37.0	37.0	37.0	27.0	37.0
39	34.714648970127165	37.0	37.0	37.0	27.0	37.0
40	34.49644944750285	37.0	37.0	37.0	27.0	37.0
41	34.68103282099546	37.0	37.0	37.0	27.0	37.0
42	34.72249987243524	37.0	37.0	37.0	27.0	37.0
43	34.314914871783245	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	14.0
16	26.0
17	30.0
18	24.0
19	37.0
20	85.0
21	260.0
22	664.0
23	1506.0
24	2889.0
25	4886.0
26	7497.0
27	10584.0
28	14510.0
29	18508.0
30	23407.0
31	29201.0
32	36711.0
33	47025.0
34	67653.0
35	128310.0
36	311693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.556947743804606	21.167529382416472	13.739716862927413	24.53580601085151
2	16.13566654004683	21.9065545608654	38.39940242996695	23.558376469120823
3	17.725973886076165	27.73442150798555	30.067722713897755	24.471881892040525
4	12.565128897103431	18.375987209506693	39.70963425765814	29.349249635731738
5	13.86784857779466	37.301353320368975	35.52877010562362	13.302027996212745
6	29.217007500807913	40.40004308854128	17.192753187701623	13.190196222949183
7	26.46614431259603	32.32533549531979	23.77339395966686	17.435126232417325
8	24.443817644757658	35.97368764209297	20.614748754117507	18.967745959031866
9	25.888701163957567	15.42909950618264	20.556210702966872	38.12598862689292
10	15.000056695449057	28.1188166525873	34.04731802178239	22.833808630181256
11	33.53068074225682	23.981891473571416	24.16756906923081	18.31985871494095
12	22.831966028086924	26.24446510678588	30.163396284180266	20.76017258094693
13	27.358247203496976	22.515038467862183	26.395983694388853	23.73073063425199
14	21.804927968431976	21.133795590227972	28.209671109700025	28.85160533164003
15	23.759787051893344	28.893418225319056	24.933808063226763	22.412986659560836
16	22.6482727731445	28.69144068805597	25.29254851713053	23.367738021669002
17	22.119729449317102	27.79919605853238	26.57103089335019	23.510043598800323
18	22.62630328663518	27.545200446760138	28.099398461285514	21.729097805319167
19	24.536939919832633	26.45749825661494	27.673473900250027	21.332087923302396
20	23.84497196410044	26.560117019406853	27.837890702513306	21.757020313979396
21	22.755994126351478	27.302543924799156	27.88296358451307	22.058498364336295
22	22.56833218997511	27.11346460219638	27.763903141494833	22.554300066333674
23	22.516597592711232	26.97782074032917	28.1831659872662	22.322415679693393
24	22.667832703068925	27.711459851117752	26.821057823688495	22.799649622124832
25	22.741678525464764	27.119984578837858	27.969849360191855	22.168487535505523
26	22.910064009161985	27.25336062274281	27.945328578475003	21.891246789620197
27	22.857904196030184	27.112614170460535	27.097022921970055	22.932458711539226
28	22.24573508484474	26.918432257442692	28.385001785906645	22.45083087180592
29	23.755960109082043	26.850822934443052	27.29772481162937	22.095492144845533
30	23.125931931443862	26.579393472085993	27.900822650965807	22.393851945504334
31	22.69051088269145	26.903124486197495	28.033915217625484	22.37244941348558
32	21.840504362714803	26.705682584858913	28.16388953458706	23.289923517839224
33	21.860631247129795	27.07051779953623	28.507747433113543	22.56110352022043
34	22.87590500110556	26.813970892556455	28.015630935304824	22.29449317103316
35	22.749332411087362	27.27518837062949	27.474614612685038	22.50086460559811
36	23.371281487235017	27.214807717384527	27.156411404856534	22.257499390523922
37	22.61127899263526	26.66968097470816	27.58786377217501	23.13117626048157
38	22.89645710138847	27.004184124140355	27.461716398024734	22.637642376446443
39	22.28145321775021	27.05960392559289	27.748170154381707	22.91077270227519
40	22.580096495654296	27.419761765723067	27.91414608149404	22.085995657128603
41	22.380953730844023	26.544525770916366	27.92038258089023	23.154137917349374
42	22.0331271508836	27.62258973472199	27.66383567391045	22.680447440483952
43	22.969735969293744	26.26572590018199	27.695868602627264	23.068669527896997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	30.5
2	35.0
3	147.0
4	259.0
5	259.0
6	340.5
7	422.0
8	418.5
9	415.0
10	559.5
11	704.0
12	704.0
13	1185.0
14	1666.0
15	2909.5
16	4153.0
17	4742.5
18	5332.0
19	5332.0
20	5249.5
21	5167.0
22	5842.5
23	6518.0
24	8494.5
25	10471.0
26	10471.0
27	13236.0
28	16001.0
29	19034.0
30	22067.0
31	25841.5
32	29616.0
33	29616.0
34	35169.0
35	40722.0
36	43774.0
37	46826.0
38	49561.5
39	52297.0
40	52297.0
41	54127.5
42	55958.0
43	56353.5
44	56749.0
45	55032.5
46	53316.0
47	53316.0
48	50936.0
49	48556.0
50	48612.5
51	48669.0
52	46232.0
53	43795.0
54	43795.0
55	42462.5
56	41130.0
57	35664.0
58	30198.0
59	27670.5
60	25143.0
61	25143.0
62	22539.5
63	19936.0
64	16364.5
65	12793.0
66	10775.0
67	8757.0
68	8757.0
69	7320.0
70	5883.0
71	5040.5
72	4198.0
73	3846.0
74	3494.0
75	3494.0
76	2854.0
77	2214.0
78	1656.0
79	1098.0
80	729.5
81	361.0
82	361.0
83	297.5
84	234.0
85	207.0
86	180.0
87	143.0
88	106.0
89	106.0
90	75.0
91	44.0
92	29.0
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	705524.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.42080610614465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.77899155053821	53.64098234774123
2	6.518360757276769	7.876892228906436
3	1.5568454791147872	2.821975764924673
4	0.701872340245275	1.69630770324903
5	0.4229042931731021	1.2776109149634076
6	0.2869722293483393	1.0403456056382452
7	0.21845949847741392	0.9239649299684599
8	0.17194971714341184	0.8311472415622803
9	0.13351782086037567	0.7260528929327742
>10	0.9994856079433472	11.90091059205794
>50	0.12424238601588869	5.309279426311635
>100	0.0799719985510114	8.788725434476289
>500	0.005712285610752241	2.261248138417204
>1k	7.140357013440302E-4	0.9045567788504334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2856	0.40480550626201234	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2161	0.30629716352668374	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1273	0.18043326662168827	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	974	0.13805341845210087	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	879	0.1245882493012286	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	821	0.11636740918806447	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	817	0.11580045469750143	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	807	0.11438306847109384	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	804	0.11395785260317154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4173862264076062E-4	0.0	0.0	0.0	0.0
2	1.4173862264076062E-4	0.0	2.8347724528152125E-4	0.0	0.0
3	1.4173862264076062E-4	0.0	5.669544905630425E-4	0.0	0.0
4	1.4173862264076062E-4	0.0	5.669544905630425E-4	0.0	0.0
5	2.8347724528152125E-4	0.0	5.669544905630425E-4	0.0	0.0
6	4.252158679222819E-4	0.0	9.921703584853244E-4	0.0	0.0
7	4.252158679222819E-4	0.0	0.0024095565848929307	0.0	0.0
8	4.252158679222819E-4	0.0	0.002693033830174452	0.0	0.0
9	4.252158679222819E-4	0.0	0.0029765110754559733	7.086931132038031E-4	0.0
10	7.086931132038031E-4	0.0	0.0036852041886597763	7.086931132038031E-4	0.0
11	7.086931132038031E-4	0.0	0.004677374547145101	8.504317358445638E-4	0.0
12	7.086931132038031E-4	0.0	0.005244329037708143	9.921703584853244E-4	0.0
13	7.086931132038031E-4	0.0	0.005527806282989664	0.001133908981126085	0.0
14	7.086931132038031E-4	0.0	0.006236499396193467	0.001133908981126085	0.0
15	7.086931132038031E-4	0.0	0.00680345388675651	0.001133908981126085	0.0
16	8.504317358445638E-4	0.0	0.007086931132038031	0.0012756476037668457	0.0
17	8.504317358445638E-4	0.0	0.0073704083773195525	0.0015591248490483668	0.0
18	9.921703584853244E-4	1.4173862264076062E-4	0.007512146999960313	0.0018426020943298881	0.0
19	9.921703584853244E-4	1.4173862264076062E-4	0.007512146999960313	0.0018426020943298881	0.0
20	9.921703584853244E-4	1.4173862264076062E-4	0.008079101490523356	0.002126079339611409	0.0
21	9.921703584853244E-4	1.4173862264076062E-4	0.008362578735804876	0.0029765110754559733	1.4173862264076062E-4
22	0.001133908981126085	1.4173862264076062E-4	0.008504317358445637	0.003968681433941298	1.4173862264076062E-4
23	0.001133908981126085	1.4173862264076062E-4	0.008504317358445637	0.004393897301863579	1.4173862264076062E-4
24	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.00453563592450434	1.4173862264076062E-4
25	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.005386067660348904	1.4173862264076062E-4
26	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.005669544905630425	1.4173862264076062E-4
27	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.0073704083773195525	1.4173862264076062E-4
28	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.01346516915087226	1.4173862264076062E-4
29	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.027497292792307563	1.4173862264076062E-4
30	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.05159285864123687	1.4173862264076062E-4
31	0.001133908981126085	1.4173862264076062E-4	0.008787794603727158	0.10431962626359982	1.4173862264076062E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	45	4.0053746E-6	28.777777	15
GCCGGCA	90	5.456968E-12	26.722221	15
GAATCGG	60	1.3361841E-6	24.666668	22
TTGGTCG	45	1.322505E-4	24.666666	10
AATCGGG	70	1.9207255E-7	23.785713	23
GGTATCA	730	0.0	23.31507	1
CCGCTCT	120	0.0	23.125002	28
CGCTCTC	120	0.0	23.125002	29
TCGGCGT	40	0.0019304522	23.125	11
TTCGCCG	115	5.456968E-12	22.52174	24
GCGGAAT	50	2.7006684E-4	22.2	19
CTTCGCC	135	0.0	21.925926	23
GCCGCTC	120	1.0913936E-11	21.583334	27
GGAATCG	70	5.097316E-6	21.142857	21
GTATCAA	1690	0.0	20.68935	1
CGGAATC	65	6.897302E-5	19.923077	20
AGCTTCG	130	3.274181E-11	19.923077	21
GCTTCGC	135	5.638867E-11	19.185186	22
TCGCCGC	155	1.8189894E-12	19.096775	25
CGGCGTA	50	0.0070322533	18.5	12
>>END_MODULE