FastQCFastQC Report
Fri 10 Feb 2017
ERR1633315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945933
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170161.7988589043832917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123541.3060121594235534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66180.6996267177485086No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA31730.3354360192529492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30980.3275073393147295No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29010.306681340010339No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG26360.278666670895296No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG24520.2592149761135303No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT21690.22929742381331447No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20280.2143915055294614No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC18380.19430551635263807No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17880.18901972972715828No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG17720.18732827800700472No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17710.18722256227449513No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.17961102953380417No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16630.17580526316345874No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16360.17295093838569964No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16290.17221092825813245No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA15560.16449367978493193No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15340.1621679336697208No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14960.15815073583435615No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14800.1564592841142026No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC14470.15297066494138592No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14150.14958776150107883No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13770.1455705636657142No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13650.144301974875599No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13390.1415533658303495No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13280.14039049277274396No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT13060.13806474665753282No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12800.13531613761228334No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12790.13521042187977372No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12600.1332018229620914No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12490.13203894990448584No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA12360.13066464538186107No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC12340.1304532139168419No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG12240.1293960565917459No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12240.1293960565917459No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG12170.12865604646417875No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT11970.1265417318139868No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11960.12643601608147723No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA11960.12643601608147723No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11940.12622458461645805No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11870.12548457448889086No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11510.12167880811854538No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT11440.12093879799097822No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT11250.11893019907329588No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11180.1181901889457287No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG11130.11766161028318074No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11090.11723874735314235No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC11090.11723874735314235No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10990.11618159002804637No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10780.11396155964534485No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10700.11311583378526809No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT10670.11279868658773931No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10650.11258725512272011No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG10640.11248153939021051No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.11153009779762416No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC10480.11079008767005698No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA10420.1101557932749994No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10300.10888720448488423No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.1071957527647307No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10090.10666717410218271No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10060.10635002690465392No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA10050.10624431117214432No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9930.10497572238202917No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9920.10487000664951956No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC9810.10370713359191402No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC9720.10275569199932764No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9680.10233282906928927No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA9630.10180425040674128No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9630.10180425040674128No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9550.10095852454666451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA200.001841771637.032
GGCCCAT200.001841771637.032
TAACTGC303.5990006E-430.8333346
CGGGTTT250.00549585829.617
CACGGGT250.00549585829.624
GATTATA250.00549585829.61
CTAAGGA405.9390364E-527.7523
CTAGTGC706.5883796E-926.4285729
AACTCTC358.868228E-426.42857219
ACTAGTG1350.026.0370358
AGATCTT451.3229703E-424.6666668
GACATCT451.3229703E-424.66666618
TAGTGCA751.3731551E-824.66666610
GTGACAT451.3229703E-424.66666616
CGGACCG451.3229703E-424.66666617
GGCAGCT6550.024.00763318
GCCGGCA6550.024.00763315
GGTATCA31100.023.7942121
CCGCTCT6150.023.76422928
CGCTCTC6150.023.76422929
Produced by FastQC (version 0.11.5)