FastQCFastQC Report
Fri 10 Feb 2017
ERR1633314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152532
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59160.5133046197415777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.348536960362055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22260.19313997355387963No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC11650.10108179208906999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.027.144861
GTATCAA31650.024.14061
CGCCGTC1250.023.6826
TTAACGG1900.022.39473735
CGTCGGA502.7021085E-422.214
CGAGTCG1450.021.68965521
CGAACCG1800.021.58333231
CCGCTCT1800.020.55555528
GTCCGGC1650.020.1818215
CGAACGC555.1431067E-420.18181830
CGCTCTC1900.019.47368429
TTCGCCG1900.019.47368424
GGCGAAC2000.019.42500129
TAACGGC2200.019.34090836
GCGAACC2050.018.95122130
CCGTCCG1501.2732926E-1118.528
AACGGCC2300.018.537
TACGAAC609.2368363E-418.528
ATGCGTT801.6166763E-518.536
ACGGTAC701.2192011E-418.53
Produced by FastQC (version 0.11.5)