##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1152532
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89282206481035	33.0	33.0	33.0	33.0	33.0
2	32.00717810872062	33.0	33.0	33.0	33.0	33.0
3	31.7280873763158	33.0	33.0	33.0	27.0	33.0
4	32.00358688522314	33.0	33.0	33.0	33.0	33.0
5	32.10453419080772	33.0	33.0	33.0	33.0	33.0
6	35.27777102935103	37.0	37.0	37.0	33.0	37.0
7	35.42365591584442	37.0	37.0	37.0	33.0	37.0
8	35.55495205339201	37.0	37.0	37.0	33.0	37.0
9	35.66018644167797	37.0	37.0	37.0	33.0	37.0
10	35.67009072199297	37.0	37.0	37.0	33.0	37.0
11	35.69739235006056	37.0	37.0	37.0	33.0	37.0
12	35.68563128832865	37.0	37.0	37.0	33.0	37.0
13	35.68835138633895	37.0	37.0	37.0	33.0	37.0
14	35.6584441907036	37.0	37.0	37.0	33.0	37.0
15	35.687444687002184	37.0	37.0	37.0	33.0	37.0
16	35.6668968844249	37.0	37.0	37.0	33.0	37.0
17	35.671681133365496	37.0	37.0	37.0	33.0	37.0
18	35.647836242290886	37.0	37.0	37.0	33.0	37.0
19	35.64415131206769	37.0	37.0	37.0	33.0	37.0
20	35.6505450607879	37.0	37.0	37.0	33.0	37.0
21	35.64250190016416	37.0	37.0	37.0	33.0	37.0
22	35.55561320640121	37.0	37.0	37.0	33.0	37.0
23	35.60063408217733	37.0	37.0	37.0	33.0	37.0
24	35.588221411639765	37.0	37.0	37.0	33.0	37.0
25	35.60842128461509	37.0	37.0	37.0	33.0	37.0
26	35.506520426330894	37.0	37.0	37.0	33.0	37.0
27	35.52312907580874	37.0	37.0	37.0	33.0	37.0
28	35.54577139723669	37.0	37.0	37.0	33.0	37.0
29	35.54922900188455	37.0	37.0	37.0	33.0	37.0
30	35.560139761846095	37.0	37.0	37.0	33.0	37.0
31	35.554904332374285	37.0	37.0	37.0	33.0	37.0
32	35.5446234898467	37.0	37.0	37.0	33.0	37.0
33	35.53492831435483	37.0	37.0	37.0	33.0	37.0
34	35.50972033748304	37.0	37.0	37.0	33.0	37.0
35	35.457178629313546	37.0	37.0	37.0	33.0	37.0
36	35.484429933398815	37.0	37.0	37.0	33.0	37.0
37	35.4765498918902	37.0	37.0	37.0	33.0	37.0
38	35.46260667816598	37.0	37.0	37.0	33.0	37.0
39	35.37311501979988	37.0	37.0	37.0	33.0	37.0
40	35.17326286818934	37.0	37.0	37.0	33.0	37.0
41	35.331425071060934	37.0	37.0	37.0	33.0	37.0
42	35.37917905967036	37.0	37.0	37.0	33.0	37.0
43	35.044307663474854	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	22.0
16	62.0
17	39.0
18	36.0
19	35.0
20	52.0
21	176.0
22	445.0
23	1122.0
24	2229.0
25	4292.0
26	7023.0
27	10581.0
28	14878.0
29	20550.0
30	27146.0
31	34888.0
32	44976.0
33	59586.0
34	85714.0
35	176122.0
36	662553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24536238473205	20.753697077391344	14.139477255295297	23.861463282581308
2	15.443475755987688	22.386536772948602	39.703799981258655	22.466187489805055
3	17.47630434556264	27.78760155900227	31.13241107405261	23.603683021382487
4	12.272631041914671	18.164181124688948	40.15272460981561	29.41046322358078
5	13.035993794532386	37.78879892271972	35.85002412080532	13.325183161942574
6	28.288325183161945	40.62290678263163	17.795774867856164	13.29299316635026
7	25.36441504444128	33.22094310613502	24.03482072515123	17.37982112427247
8	24.03421336674383	36.08255562535357	21.128003387324604	18.755227620577998
9	25.882925593389167	15.85552505266665	20.639600462286513	37.62194889165767
10	14.401769321806249	28.647187236449835	34.46021455369569	22.490828888048227
11	32.45133323846973	24.595152238723088	24.777967119351132	18.175547403456044
12	22.3242391534465	26.47475297865916	30.839143728764146	20.36186413913019
13	27.316291434858208	22.982095074149786	27.160460620616174	22.541152870375832
14	21.814665449636106	21.829589113360846	28.577601316058903	27.778144120944148
15	23.296272901750235	29.08682795792221	25.609614310058205	22.007284830269356
16	22.11669610908851	29.47163289175485	25.699243057893405	22.712427941263236
17	21.83878625495865	28.124338413163365	26.921682001020365	23.115193330857625
18	22.435993100408492	27.59263951022618	28.46072820537738	21.51063918398795
19	24.286093574842173	26.717609576133245	28.67903017009506	20.31726667892952
20	24.006448410976873	26.43805117775472	28.64397691343928	20.911523497829126
21	22.175262812659433	27.802178160779917	27.911676205085843	22.110882821474807
22	21.868112989487493	27.631857510247006	28.058483408703623	22.441546091561886
23	21.810761002731375	27.531990434972737	28.62957384263517	22.027674719660713
24	22.458638892455916	27.739707010304272	27.904908497117653	21.896745600122166
25	22.448660861477165	27.242280474641916	28.53196267001697	21.777095993863945
26	22.34393491894368	27.383968514540157	28.286416342452963	21.98568022406319
27	22.33890252071092	27.35151822248753	27.871156722763445	22.4384225340381
28	21.60677534333103	27.557673019057173	28.608489829349644	22.227061808262157
29	22.687960073993608	27.620230935019592	27.88321712542472	21.808591865562086
30	22.252397330399504	27.636976673966533	28.224292253924403	21.886333741709556
31	22.389400034012073	27.482273811052533	28.321296068135197	21.807030086800193
32	21.498318484866363	27.293645642810787	28.550964311619982	22.65707156070287
33	21.693714361076307	27.22223764719765	28.972731342817383	22.111316648908662
34	21.992100870084304	27.129658872812207	28.40077325401811	22.47746700308538
35	22.151315538310435	27.870896426303133	28.134316444142115	21.84347159124432
36	22.558679498703725	27.52661097479289	27.933280811292004	21.98142871521138
37	22.670693742126033	26.866672682407085	27.98690188211694	22.475731693349946
38	22.67650702973974	27.32331943928672	27.947944178556433	22.052229352417115
39	22.234870702071614	27.25269233305453	28.143600351226688	22.36883661364717
40	22.058216171004364	27.49667688185664	28.433483842531054	22.011623104607942
41	21.979433109015627	26.99161498335838	28.556604068260143	22.472347839365845
42	22.05700145418956	27.20245511621369	28.345503638944514	22.395039790652234
43	22.10264010023149	26.783117518645906	28.375264200907218	22.738978180215387
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	88.0
2	116.0
3	367.5
4	619.0
5	619.0
6	763.0
7	907.0
8	841.0
9	775.0
10	1085.0
11	1395.0
12	1395.0
13	2192.0
14	2989.0
15	5659.5
16	8330.0
17	9666.5
18	11003.0
19	11003.0
20	10824.0
21	10645.0
22	12466.0
23	14287.0
24	18072.5
25	21858.0
26	21858.0
27	26553.0
28	31248.0
29	38719.5
30	46191.0
31	50445.0
32	54699.0
33	54699.0
34	60428.0
35	66157.0
36	69648.0
37	73139.0
38	78127.5
39	83116.0
40	83116.0
41	86112.0
42	89108.0
43	85815.5
44	82523.0
45	82539.0
46	82555.0
47	82555.0
48	80153.0
49	77751.0
50	79501.5
51	81252.0
52	80587.5
53	79923.0
54	79923.0
55	71729.5
56	63536.0
57	56144.0
58	48752.0
59	42900.0
60	37048.0
61	37048.0
62	32454.0
63	27860.0
64	23172.0
65	18484.0
66	15838.5
67	13193.0
68	13193.0
69	10697.5
70	8202.0
71	7263.0
72	6324.0
73	5117.0
74	3910.0
75	3910.0
76	2876.0
77	1842.0
78	1423.0
79	1004.0
80	780.0
81	556.0
82	556.0
83	504.5
84	453.0
85	403.0
86	353.0
87	274.5
88	196.0
89	196.0
90	163.5
91	131.0
92	85.5
93	40.0
94	21.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1152532.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.02496308924084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.81772765665482	50.07802678201746
2	7.201166231669576	8.212924771208902
3	1.7102624690851012	2.9258296251747535
4	0.7701279697196132	1.756660761890117
5	0.44014032723793733	1.2549492957414883
6	0.30801185208339493	1.0538618697662583
7	0.22712148735689539	0.9066116103310309
8	0.16512586065774165	0.7533036887269475
9	0.12637847138659009	0.6486054899485483
>10	0.9690327377663748	11.105058577536939
>50	0.14233746340759734	5.719816116477378
>100	0.1164005568829133	12.163411443449752
>500	0.004933532873267279	1.8721054630529321
>1k	0.0010792103160272171	1.028717292344433
>5k	1.5417290228960246E-4	0.5201172123331054
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5916	0.5133046197415777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4017	0.348536960362055	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2226	0.19313997355387963	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1165	0.10108179208906999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.676548677173388E-5	8.676548677173388E-5	0.0
2	0.0	0.0	2.602964603152017E-4	1.7353097354346777E-4	0.0
3	0.0	0.0	3.4706194708693553E-4	1.7353097354346777E-4	0.0
4	0.0	0.0	3.4706194708693553E-4	2.602964603152017E-4	0.0
5	0.0	0.0	3.4706194708693553E-4	2.602964603152017E-4	0.0
6	0.0	0.0	8.676548677173389E-4	2.602964603152017E-4	0.0
7	0.0	0.0	0.0019956061957498794	3.4706194708693553E-4	0.0
8	0.0	0.0	0.002516199116380283	3.4706194708693553E-4	0.0
9	0.0	0.0	0.0033838539840976216	0.0011279513280325405	0.0
10	8.676548677173388E-5	0.0	0.003817681417956291	0.0011279513280325405	0.0
11	8.676548677173388E-5	0.0	0.006073584074021372	0.0012147168148042744	0.0
12	8.676548677173388E-5	0.0	0.006941238941738711	0.0012147168148042744	0.0
13	8.676548677173388E-5	0.0	0.007288300888825646	0.0012147168148042744	0.0
14	8.676548677173388E-5	0.0	0.007722128322684316	0.0012147168148042744	0.0
15	8.676548677173388E-5	0.0	0.008416252216858187	0.0012147168148042744	0.0
16	8.676548677173388E-5	0.0	0.009023610624260324	0.0012147168148042744	0.0
17	8.676548677173388E-5	0.0	0.00937067257134726	0.0012147168148042744	0.0
18	1.7353097354346777E-4	0.0	0.009717734518434195	0.0013014823015760084	0.0
19	1.7353097354346777E-4	0.0	0.009804500005205928	0.0013882477883477421	0.0
20	1.7353097354346777E-4	0.0	0.010064796465521131	0.001475013275119476	0.0
21	1.7353097354346777E-4	0.0	0.010585389386151535	0.0017353097354346778	0.0
22	1.7353097354346777E-4	0.0	0.010845685846466736	0.0019956061957498794	0.0
23	1.7353097354346777E-4	0.0	0.010932451333238469	0.0026029646031520168	0.0
24	1.7353097354346777E-4	0.0	0.010932451333238469	0.00312355752378242	0.0
25	1.7353097354346777E-4	0.0	0.010932451333238469	0.0033838539840976216	0.0
26	1.7353097354346777E-4	0.0	0.010932451333238469	0.003817681417956291	0.0
27	1.7353097354346777E-4	0.0	0.010932451333238469	0.005379460179847501	0.0
28	1.7353097354346777E-4	0.0	0.010932451333238469	0.014489836290879559	0.0
29	1.7353097354346777E-4	0.0	0.010932451333238469	0.031062044264280733	0.0
30	1.7353097354346777E-4	0.0	0.010932451333238469	0.05405489825879021	0.0
31	1.7353097354346777E-4	0.0	0.010932451333238469	0.12095108855979704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1070	0.0	27.14486	1
GTATCAA	3165	0.0	24.1406	1
CGCCGTC	125	0.0	23.68	26
TTAACGG	190	0.0	22.394737	35
CGTCGGA	50	2.7021085E-4	22.2	14
CGAGTCG	145	0.0	21.689655	21
CGAACCG	180	0.0	21.583332	31
CCGCTCT	180	0.0	20.555555	28
GTCCGGC	165	0.0	20.18182	15
CGAACGC	55	5.1431067E-4	20.181818	30
CGCTCTC	190	0.0	19.473684	29
TTCGCCG	190	0.0	19.473684	24
GGCGAAC	200	0.0	19.425001	29
TAACGGC	220	0.0	19.340908	36
GCGAACC	205	0.0	18.951221	30
CCGTCCG	150	1.2732926E-11	18.5	28
AACGGCC	230	0.0	18.5	37
TACGAAC	60	9.2368363E-4	18.5	28
ATGCGTT	80	1.6166763E-5	18.5	36
ACGGTAC	70	1.2192011E-4	18.5	3
>>END_MODULE