FastQCFastQC Report
Fri 10 Feb 2017
ERR1633313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855303
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT278353.254402241077139No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT210872.4654420714062737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116081.3571798532216068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51870.6064517486785385No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG46850.5477590982377005No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA35120.410614717825145No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA33910.39646768455155657No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30050.3513374792325059No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG29680.3470115269091772No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG28750.33613818728567535No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28370.3316953173319865No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA27250.3186005427316401No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT24660.2883188764683393No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT23770.2779132073662784No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA22890.26762445589457773No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC21730.25406201077279045No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA21390.25008681134054245No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA20250.2367582014794757No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18870.2206235684897633No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17870.20893180545373977No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.20320284156608828No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17230.20144907711068474No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16790.19630470137483444No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC16410.1918618314211455No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA16310.19069265511754313No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.18262533862268693No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC15500.1812223270583641No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC15410.180170068385122No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15030.17572719843143306No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT14970.17502569264927167No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA14390.16824447008837803No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA14170.16567228222045285No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14130.16520461169901193No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13820.16158016515784465No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.15994331833280134No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13630.15935873018100016No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13520.1580726362470376No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12960.15152524894686442No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC12900.15082374316470304No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG12830.1500053197521814No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC12820.14988840212182114No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG12760.14918689633965976No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12730.14883614344857904No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA12470.14579628505921294No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA12440.14544553216813222No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC12140.1419380032573252No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12060.1410026622144433No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12040.14076882695372284No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT11910.13924889775903979No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11740.13726129804291579No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG11380.13305226334994733No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11380.13305226334994733No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC11270.13176616941598474No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11090.1296616520695005No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10990.12849247576589815No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT10940.127907887614097No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT10900.12744021709265604No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10760.12580337026761276No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT10580.12369885292112853No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10530.12311426476932735No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10330.12077591216212266No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10270.12007440637996127No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10030.11726838325131561No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9990.11680071272987469No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA9970.11656687746915421No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9760.11411160723158928No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9760.11411160723158928No Hit
TATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATGA9470.11072099595114246No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.10966873727790034No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9330.10908414912609916No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9320.10896723149573895No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9240.10803189045285706No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9220.10779805519213659No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9170.10721346704033541No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9130.10674579651889447No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9080.10616120836709331No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8980.10499203206349095No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC8970.10487511443313072No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG8970.10487511443313072No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8750.10230292656520554No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.1014845031526839No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG8660.10125066789196344No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA8640.10101683263124296No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA8570.10019840921872132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACG200.001841672737.01
ACAACGC555.002221E-1033.6363643
AGCAACG352.3875993E-531.7142852
CCGTTTG303.5987163E-430.83333223
GTACAAC250.00549556629.61
TAACGTG250.00549556629.64
CGTTAAA250.00549556629.617
GGAACCT250.00549556629.619
ACATGTG250.00549556629.623
ACCACCA250.00549556629.625
TTAGTAG250.00549556629.63
CCTCCAC454.0066807E-628.7777791
AACAACG405.9383798E-527.752
TAGCAAC405.9383798E-527.751
TGTCAAC706.5865606E-926.428572
ATGTGTC358.867533E-426.4285725
CCACTAG358.867533E-426.428571
GTGTCAC358.867533E-426.4285727
GGGACGA451.3228253E-424.66666818
GCAGAAC451.3228253E-424.6666683
Produced by FastQC (version 0.11.5)