FastQCFastQC Report
Fri 10 Feb 2017
ERR1633312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848531
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39830.46939946802179294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.34306348265413994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15700.18502565021195455No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12990.15308810167218406No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11550.13611759617503663No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10810.12739664196122477No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10640.1253931795067004No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10480.12350756778479513No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10270.12103270239979447No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9170.10806912181169574No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9070.10689061448550495No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8910.10500500276359968No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8650.10194088371550361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAC303.5986924E-430.83333232
CGCTATC400.001930772423.12500212
GGACCGT400.001930772423.1250026
GGTATCA9550.022.4712031
CGCGATA753.738387E-722.234
TCTATCG753.738387E-722.231
CGGTGCT1450.021.68965534
GTATCAA21800.021.0458721
TATCGCC806.955688E-720.812533
ATCGCTA450.003824995420.55555510
TTAGCCG1450.020.41379429
CGCCTAT951.6748163E-719.47368436
CCGGTGC1551.8189894E-1219.09677533
TAGCCGG1650.019.06060630
GTTAGCC1601.8189894E-1218.528
TATTGCG1002.8727118E-718.511
GCGTCTA609.234102E-418.522
CGGAGTT1653.6379788E-1217.93939424
TTGCGCC1156.399023E-817.69565213
CGCCAGG1054.793601E-717.6190516
Produced by FastQC (version 0.11.5)