##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633312.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	848531
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246737007840608	33.0	33.0	33.0	27.0	33.0
2	31.515123195263342	33.0	33.0	33.0	27.0	33.0
3	31.241920448398467	33.0	33.0	33.0	27.0	33.0
4	31.570174807991695	33.0	33.0	33.0	27.0	33.0
5	31.672918255196333	33.0	33.0	33.0	27.0	33.0
6	34.599305152080476	37.0	37.0	37.0	27.0	37.0
7	34.790068954463656	37.0	37.0	37.0	27.0	37.0
8	34.928004987443	37.0	37.0	37.0	27.0	37.0
9	35.05483123185835	37.0	37.0	37.0	27.0	37.0
10	35.087682123575924	37.0	37.0	37.0	33.0	37.0
11	35.06631107172278	37.0	37.0	37.0	33.0	37.0
12	35.09442554249639	37.0	37.0	37.0	33.0	37.0
13	35.07530661814359	37.0	37.0	37.0	33.0	37.0
14	35.09818498086693	37.0	37.0	37.0	33.0	37.0
15	35.068043477492274	37.0	37.0	37.0	33.0	37.0
16	35.04309212038217	37.0	37.0	37.0	27.0	37.0
17	35.049253356683494	37.0	37.0	37.0	27.0	37.0
18	35.04073510572979	37.0	37.0	37.0	27.0	37.0
19	35.02219364996682	37.0	37.0	37.0	27.0	37.0
20	35.03011911173546	37.0	37.0	37.0	27.0	37.0
21	35.03188451571009	37.0	37.0	37.0	27.0	37.0
22	34.931344877205426	37.0	37.0	37.0	27.0	37.0
23	34.97356372365889	37.0	37.0	37.0	27.0	37.0
24	34.965013653007375	37.0	37.0	37.0	27.0	37.0
25	34.96559583562651	37.0	37.0	37.0	27.0	37.0
26	34.85767284872326	37.0	37.0	37.0	27.0	37.0
27	34.868331268981336	37.0	37.0	37.0	27.0	37.0
28	34.88210566260985	37.0	37.0	37.0	27.0	37.0
29	34.88746315691471	37.0	37.0	37.0	27.0	37.0
30	34.88299661414845	37.0	37.0	37.0	27.0	37.0
31	34.872106027947126	37.0	37.0	37.0	27.0	37.0
32	34.848363819353686	37.0	37.0	37.0	27.0	37.0
33	34.86308455436513	37.0	37.0	37.0	27.0	37.0
34	34.8317786857522	37.0	37.0	37.0	27.0	37.0
35	34.77035016988183	37.0	37.0	37.0	27.0	37.0
36	34.79234465211053	37.0	37.0	37.0	27.0	37.0
37	34.790735989610276	37.0	37.0	37.0	27.0	37.0
38	34.768146361181856	37.0	37.0	37.0	27.0	37.0
39	34.67687921831966	37.0	37.0	37.0	27.0	37.0
40	34.45062466780825	37.0	37.0	37.0	27.0	37.0
41	34.63808747117076	37.0	37.0	37.0	27.0	37.0
42	34.67077455036999	37.0	37.0	37.0	27.0	37.0
43	34.27197945626029	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	16.0
16	28.0
17	33.0
18	24.0
19	52.0
20	116.0
21	338.0
22	832.0
23	1878.0
24	3659.0
25	6003.0
26	9154.0
27	13134.0
28	17696.0
29	22629.0
30	28948.0
31	35444.0
32	44601.0
33	58097.0
34	82165.0
35	155820.0
36	367858.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.88336195141957	20.960931303629447	13.6119953189689	24.543711425982078
2	16.311837752539386	21.82336296493587	38.20119712774194	23.663602154782794
3	17.633180166664506	27.854020654519402	30.044630072442846	24.46816910637325
4	12.52529371348837	18.44187189389663	39.693540954897344	29.339293437717657
5	13.807273982918714	37.37082086570791	35.47637033885621	13.345534812517162
6	29.023807026496378	40.478426834140414	17.36766246607372	13.130103673289483
7	26.499090781597843	32.37383195192633	23.805965839786644	17.321111426689185
8	24.45885889849634	35.88436957518347	20.62505671566507	19.03171481065512
9	26.09120939600321	15.391187829319142	20.490824731211944	38.02677804346571
10	15.30539249597245	28.079586956752316	33.80230068200219	22.812719865273042
11	33.53324745943283	23.930298362699773	24.261458921359385	18.27499525650801
12	23.032865033805482	26.201989084665144	29.78971893778778	20.975426943741596
13	27.321806746011635	22.518328735190583	26.26044304804421	23.899421470753573
14	21.859660990582547	21.161984653477596	28.03044320125016	28.94791115468969
15	24.07372270429719	28.549929230635062	24.96868116780648	22.407666897261265
16	22.725038920204447	28.503731743448384	25.336964707241105	23.434264629106067
17	22.169961969568583	27.650138887088392	26.559076804500954	23.62082233884207
18	22.66988477733872	27.38414978356713	28.08359388166137	21.862371557432787
19	24.76527080330595	26.25231134749349	27.651435245147198	21.330982604053357
20	23.913563558667864	26.41023132920306	27.98813478823991	21.688070323889168
21	22.827686908315663	27.243553859552566	27.851545789134395	22.07721344299737
22	22.729752949509212	26.987699919036544	27.65214234954292	22.630404781911327
23	22.378557766304354	26.96318696665178	28.40980470955098	22.24845055749289
24	22.968989936725944	27.48644421948049	26.70167619097004	22.84288965282353
25	22.950369520972128	26.936670551812487	28.0152404567423	22.097719470473088
26	23.017662289297622	27.292579764322106	27.89326494848155	21.796492997898724
27	22.947894655587124	27.08221620659705	27.012095020688697	22.95779411712713
28	22.148041733301437	26.876330976711515	28.542858186677915	22.43276910330913
29	23.978145760143118	26.62224479718478	27.355040652610217	22.044568790061884
30	23.16839337631742	26.45041842902616	28.006519502528487	22.374668692127926
31	22.701940176611107	26.811159521573163	27.996855742453725	22.490044559362005
32	21.82100595028349	26.51217221291856	28.10221429741518	23.564607539382767
33	21.803917594053722	26.826597967546267	28.599426538335077	22.770057900064934
34	22.98737465101452	26.558723252303096	28.115767131666374	22.33813496501601
35	22.853496218759243	27.288572839413057	27.547373048244555	22.310557893583148
36	23.741737190509244	27.016337647062983	26.98216093460345	22.259764227824324
37	22.870231022791152	26.47363502335212	27.44425365720286	23.211880296653863
38	23.020490706880476	26.93207437324034	27.347498205722598	22.699936714156582
39	22.257289362439323	27.073259550918	27.793327527220573	22.87612355942211
40	22.559105088676784	27.384385485032368	28.007462308389442	22.049047117901406
41	22.244325781851224	26.476227739469742	28.003455383480393	23.27599109519864
42	22.02076294207283	27.529106184688594	27.619144144409574	22.830986728829
43	23.044532256334772	26.163569745831328	27.73711272776127	23.05478527007263
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	34.5
2	50.0
3	190.0
4	330.0
5	330.0
6	443.0
7	556.0
8	572.0
9	588.0
10	760.0
11	932.0
12	932.0
13	1508.0
14	2084.0
15	3741.5
16	5399.0
17	6122.5
18	6846.0
19	6846.0
20	6227.0
21	5608.0
22	6004.0
23	6400.0
24	8535.0
25	10670.0
26	10670.0
27	13706.0
28	16742.0
29	20034.5
30	23327.0
31	28130.5
32	32934.0
33	32934.0
34	40706.5
35	48479.0
36	52474.0
37	56469.0
38	60168.5
39	63868.0
40	63868.0
41	67036.5
42	70205.0
43	70827.5
44	71450.0
45	69385.0
46	67320.0
47	67320.0
48	63842.5
49	60365.0
50	60751.0
51	61137.0
52	57690.0
53	54243.0
54	54243.0
55	52572.0
56	50901.0
57	43251.0
58	35601.0
59	32539.5
60	29478.0
61	29478.0
62	26185.0
63	22892.0
64	18446.5
65	14001.0
66	11684.0
67	9367.0
68	9367.0
69	7903.5
70	6440.0
71	5516.0
72	4592.0
73	4356.0
74	4120.0
75	4120.0
76	3415.0
77	2710.0
78	2023.0
79	1336.0
80	886.0
81	436.0
82	436.0
83	352.5
84	269.0
85	242.0
86	215.0
87	155.5
88	96.0
89	96.0
90	69.0
91	42.0
92	25.5
93	9.0
94	6.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	848531.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.51948471793988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.55836751745807	45.46000860042963
2	7.446851885276414	7.822096475710729
3	1.9238217176837515	3.0311437590579797
4	0.9168639647573313	1.9261289194200977
5	0.5640668702507134	1.481225068601426
6	0.38587099698846156	1.215944755765904
7	0.2767981976712821	1.0176109098785142
8	0.21280460098666662	0.8941110391541225
9	0.1528624505658232	0.7225431422794734
>10	1.258738719394743	13.306440591225313
>50	0.16742177322355387	6.183978362792742
>100	0.12459768780144517	12.034502530533471
>500	0.008883564577702096	3.0932943519468017
>1k	0.002050053364085099	1.8109714932038012
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3983	0.46939946802179294	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2911	0.34306348265413994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1570	0.18502565021195455	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1299	0.15308810167218406	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1155	0.13611759617503663	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1081	0.12739664196122477	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1064	0.1253931795067004	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1048	0.12350756778479513	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1027	0.12103270239979447	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	917	0.10806912181169574	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	907	0.10689061448550495	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	891	0.10500500276359968	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	865	0.10194088371550361	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.1785073261907933E-4	0.0	0.0
4	0.0	0.0	1.1785073261907933E-4	2.3570146523815865E-4	0.0
5	0.0	1.1785073261907933E-4	2.3570146523815865E-4	2.3570146523815865E-4	0.0
6	0.0	1.1785073261907933E-4	0.0011785073261907932	2.3570146523815865E-4	1.1785073261907933E-4
7	0.0	1.1785073261907933E-4	0.0030641190480960624	2.3570146523815865E-4	1.1785073261907933E-4
8	0.0	1.1785073261907933E-4	0.0038890741764296176	3.53552197857238E-4	1.1785073261907933E-4
9	0.0	1.1785073261907933E-4	0.005067581502620411	0.0015320595240480312	1.1785073261907933E-4
10	1.1785073261907933E-4	1.1785073261907933E-4	0.0058925366309539665	0.0016499102566671106	4.714029304763173E-4
11	2.3570146523815865E-4	1.1785073261907933E-4	0.00883880494643095	0.0018856117219052692	4.714029304763173E-4
12	2.3570146523815865E-4	1.1785073261907933E-4	0.009781610807383585	0.0018856117219052692	4.714029304763173E-4
13	2.3570146523815865E-4	1.1785073261907933E-4	0.01048871520309806	0.0018856117219052692	4.714029304763173E-4
14	2.3570146523815865E-4	1.1785073261907933E-4	0.010842267400955298	0.0018856117219052692	4.714029304763173E-4
15	2.3570146523815865E-4	1.1785073261907933E-4	0.011667222529288854	0.0018856117219052692	5.892536630953966E-4
16	2.3570146523815865E-4	1.1785073261907933E-4	0.012374326925003329	0.0018856117219052692	5.892536630953966E-4
17	2.3570146523815865E-4	1.1785073261907933E-4	0.012727879122860566	0.0018856117219052692	5.892536630953966E-4
18	2.3570146523815865E-4	1.1785073261907933E-4	0.012963580588098727	0.0018856117219052692	5.892536630953966E-4
19	2.3570146523815865E-4	1.1785073261907933E-4	0.013199282053336885	0.0018856117219052692	5.892536630953966E-4
20	2.3570146523815865E-4	1.1785073261907933E-4	0.013552834251194123	0.002121313187143428	5.892536630953966E-4
21	2.3570146523815865E-4	1.1785073261907933E-4	0.013788535716432281	0.002474865385000666	7.07104395714476E-4
22	2.3570146523815865E-4	1.1785073261907933E-4	0.01390638644905136	0.002828417582857904	7.07104395714476E-4
23	2.3570146523815865E-4	1.1785073261907933E-4	0.01402423718167044	0.0031819697807151416	7.07104395714476E-4
24	2.3570146523815865E-4	1.1785073261907933E-4	0.01402423718167044	0.0036533727111914592	7.07104395714476E-4
25	2.3570146523815865E-4	1.1785073261907933E-4	0.014142087914289518	0.004124775641667776	7.07104395714476E-4
26	2.3570146523815865E-4	1.1785073261907933E-4	0.014142087914289518	0.0044783278395250144	7.07104395714476E-4
27	2.3570146523815865E-4	1.1785073261907933E-4	0.014142087914289518	0.00530328296785857	7.07104395714476E-4
28	2.3570146523815865E-4	1.1785073261907933E-4	0.014142087914289518	0.014259938646908598	7.07104395714476E-4
29	2.3570146523815865E-4	1.1785073261907933E-4	0.014142087914289518	0.03240895147024681	7.07104395714476E-4
30	2.3570146523815865E-4	1.1785073261907933E-4	0.014142087914289518	0.05810041118120611	7.07104395714476E-4
31	3.53552197857238E-4	1.1785073261907933E-4	0.014142087914289518	0.12268261265646158	7.07104395714476E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAC	30	3.5986924E-4	30.833332	32
CGCTATC	40	0.0019307724	23.125002	12
GGACCGT	40	0.0019307724	23.125002	6
GGTATCA	955	0.0	22.471203	1
CGCGATA	75	3.738387E-7	22.2	34
TCTATCG	75	3.738387E-7	22.2	31
CGGTGCT	145	0.0	21.689655	34
GTATCAA	2180	0.0	21.045872	1
TATCGCC	80	6.955688E-7	20.8125	33
ATCGCTA	45	0.0038249954	20.555555	10
TTAGCCG	145	0.0	20.413794	29
CGCCTAT	95	1.6748163E-7	19.473684	36
CCGGTGC	155	1.8189894E-12	19.096775	33
TAGCCGG	165	0.0	19.060606	30
GTTAGCC	160	1.8189894E-12	18.5	28
TATTGCG	100	2.8727118E-7	18.5	11
GCGTCTA	60	9.234102E-4	18.5	22
CGGAGTT	165	3.6379788E-12	17.939394	24
TTGCGCC	115	6.399023E-8	17.695652	13
CGCCAGG	105	4.793601E-7	17.61905	16
>>END_MODULE