FastQCFastQC Report
Fri 10 Feb 2017
ERR1633311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960711
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT279442.908679092880169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT212542.2123198339563093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108331.1276023694950927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.5506338534689412No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA52800.5495929577156918No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA45300.4715257762219856No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG45220.47069305961938607No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT43450.45226920478687144No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG42160.438841649569954No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC37470.3900236387425563No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA35390.36837300707496845No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG31000.32267768350731907No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA29630.30841741168780207No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.30748060550987755No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA26960.2806254950760426No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT25870.269279731365624No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC25220.2625139089695028No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC24520.25522763869675685No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC23970.2495027120538851No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC23580.24544321861621238No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT23100.24044691900061516No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22720.23649151513826738No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC22100.230037961468121No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA21470.2234803182226497No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG21170.22035763096290142No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC21160.2202535413875765No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20600.21442452516937976No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA19810.20620144871870938No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC19650.2045360155135103No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA19340.2013092386784371No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA19000.1977701931173891No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG18700.19464750585764085No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT18600.19360661010439142No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.18881848963944411No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC17880.18611216068099562No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17770.1849671753524213No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17580.18298947342124738No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17320.18028314446279892No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17250.1795545174355243No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG17170.17872180083292477No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT16880.1757032031485015No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC16840.1752868448472017No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC16790.174766396970577No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG16470.17143553056017888No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT16410.17081099310822923No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC16400.17070690353290427No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT15700.16342063326015838No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG15680.16321245410950846No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT15480.16113066260300965No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC15390.16019385642508518No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.1585284232198861No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15080.156967079590012No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA15000.15613436298741243No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC14940.1555098255354628No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG14850.15457301935753834No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA14620.15217895912506468No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14540.15134624252246515No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG14440.15030534676921573No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA14380.14968080931726607No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA14340.1492644510159663No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14190.14770310738609216No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG14150.1472867490847924No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG14110.14687039078349265No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG14020.14593358460556816No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA13920.14489268885231874No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG13880.14447633055101897No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG13700.14260271819517004No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG13630.14187409116789546No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT13600.14156182244192062No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13450.1400004788120465No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13100.13635734367567354No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG13040.13573280622372388No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12820.13344283556657519No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12490.13000787958085208No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT12380.12886289425227776No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC12360.12865471510162785No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12150.12646883401980408No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG12090.12584429656785442No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA12070.12563611741720457No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG11960.12449113208863019No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA11770.12251343015745629No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG11630.12105617610290711No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC11570.12043163865095748No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA11230.11689259308990944No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC11090.11543533903536027No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC10850.11293718922756169No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC10700.11137584559768754No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10610.11043903941976307No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG10590.11023086026911318No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT10530.10960632281716354No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT10490.10918996451586378No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10430.10856542706391412No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC10210.1062754564067654No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA10210.1062754564067654No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA10050.10461002320156633No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG10030.10440184405091646No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC9950.10356912744831694No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA9850.10252823169506751No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9780.1017996046677929No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9700.10096688806519338No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG9680.10075870891454351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATAA250.005495899429.61
AAATTGC250.005495899429.629
GCTCGTC250.005495899429.611
TGTCAAC454.0073573E-628.7777772
ACAACGC604.3211912E-827.7500023
ACCAAGA405.9391306E-527.7512
AACAACG556.259961E-726.909092
TAACAAC556.259961E-726.909091
GTGACAT1250.026.64000116
AGCAACG358.868333E-426.428572
TAGCAAC358.868333E-426.428571
TACTATC358.868333E-426.4285726
AATACTA358.868333E-426.4285724
GTATGCC509.089059E-625.90000237
GGTATCA42350.025.7733151
TCTTGGT1300.025.61538711
GATCTTG1300.025.6153879
AAGCTTC809.786163E-1025.437536
TAGATCT1250.025.1600027
TATAGAT1250.025.1600025
Produced by FastQC (version 0.11.5)