FastQCFastQC Report
Fri 10 Feb 2017
ERR1633310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082843
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68320.630931723250739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47650.44004532513023586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25590.23632234774570274No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA14460.1335373641423549No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.11100408831197135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.025.4981271
ACGTCCG400.001931115523.12532
GTATCAA35850.021.931661
GCCGCTC3850.021.14285927
CGGATGC705.101472E-621.1428599
CCGCTCT3750.020.72000128
GCCGGCA4050.020.55555515
CGTCCGG450.00382566820.55555533
TTCGCCG3800.020.44736924
CCGGCAG4200.020.26190416
TCTGTCG656.9016576E-519.9230778
AGCTTCG4250.019.58823621
CGCCGGC4350.019.5632214
TTGCGGT1254.129106E-1019.24000235
CGCTCTC4250.019.1529429
CGAGTCG1551.8189894E-1219.09677321
CTCACGA500.00703462518.529
TACCCGA701.2191145E-418.530
TTAACGG2200.018.535
ATACGTC609.2363456E-418.529
Produced by FastQC (version 0.11.5)