##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633310.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1082843
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848593932823132	33.0	33.0	33.0	33.0	33.0
2	31.973449521306414	33.0	33.0	33.0	33.0	33.0
3	31.69739749899108	33.0	33.0	33.0	27.0	33.0
4	31.979141020443407	33.0	33.0	33.0	33.0	33.0
5	32.08374067154703	33.0	33.0	33.0	33.0	33.0
6	35.26670348333045	37.0	37.0	37.0	33.0	37.0
7	35.41317347020759	37.0	37.0	37.0	33.0	37.0
8	35.54197976992048	37.0	37.0	37.0	33.0	37.0
9	35.645194178657476	37.0	37.0	37.0	33.0	37.0
10	35.65825886116455	37.0	37.0	37.0	33.0	37.0
11	35.686910290780844	37.0	37.0	37.0	33.0	37.0
12	35.66610117994945	37.0	37.0	37.0	33.0	37.0
13	35.67548019426639	37.0	37.0	37.0	33.0	37.0
14	35.64320127663937	37.0	37.0	37.0	33.0	37.0
15	35.674339678051204	37.0	37.0	37.0	33.0	37.0
16	35.66577333925601	37.0	37.0	37.0	33.0	37.0
17	35.644966075414445	37.0	37.0	37.0	33.0	37.0
18	35.636126382125575	37.0	37.0	37.0	33.0	37.0
19	35.63168991257274	37.0	37.0	37.0	33.0	37.0
20	35.622234248178174	37.0	37.0	37.0	33.0	37.0
21	35.62542399960105	37.0	37.0	37.0	33.0	37.0
22	35.53526873240165	37.0	37.0	37.0	33.0	37.0
23	35.5771917073851	37.0	37.0	37.0	33.0	37.0
24	35.5647328375397	37.0	37.0	37.0	33.0	37.0
25	35.59607440783198	37.0	37.0	37.0	33.0	37.0
26	35.48405724560255	37.0	37.0	37.0	33.0	37.0
27	35.499161928368196	37.0	37.0	37.0	33.0	37.0
28	35.51428877501171	37.0	37.0	37.0	33.0	37.0
29	35.520606403698416	37.0	37.0	37.0	33.0	37.0
30	35.52748736428088	37.0	37.0	37.0	33.0	37.0
31	35.51763182658982	37.0	37.0	37.0	33.0	37.0
32	35.51153583668177	37.0	37.0	37.0	33.0	37.0
33	35.50293625206978	37.0	37.0	37.0	33.0	37.0
34	35.47845901945157	37.0	37.0	37.0	33.0	37.0
35	35.40753276329071	37.0	37.0	37.0	33.0	37.0
36	35.4390756554736	37.0	37.0	37.0	33.0	37.0
37	35.43612785971743	37.0	37.0	37.0	33.0	37.0
38	35.4194162958065	37.0	37.0	37.0	33.0	37.0
39	35.31429579357303	37.0	37.0	37.0	33.0	37.0
40	35.09430268284507	37.0	37.0	37.0	33.0	37.0
41	35.26579568783286	37.0	37.0	37.0	33.0	37.0
42	35.30318799678254	37.0	37.0	37.0	33.0	37.0
43	34.96979063446871	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	10.0
15	33.0
16	57.0
17	55.0
18	52.0
19	39.0
20	64.0
21	168.0
22	468.0
23	1143.0
24	2269.0
25	4082.0
26	6666.0
27	10202.0
28	14422.0
29	19651.0
30	26104.0
31	33419.0
32	43023.0
33	56726.0
34	82041.0
35	167229.0
36	614920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06981067430828	20.09395637225341	13.948005389516302	23.88822756392201
2	15.994470112472445	21.990814919614383	38.885969618864415	23.128745349048753
3	17.879230876498255	27.67243266105982	30.45510752712997	23.993228935311954
4	12.174156364311354	17.873228159576225	40.34730796615945	29.605307509952965
5	13.816961461633865	36.80127220658951	35.2074123395543	14.174353992222327
6	28.659464022023506	40.60754883210216	17.184485654891798	13.548501490982535
7	26.02371719630639	32.77363385089067	23.42851179718574	17.774137155617204
8	24.894005871580642	34.831826959217544	20.843464842086988	19.430702327114826
9	26.698607277324598	15.056845729251608	20.24744122647512	37.99710576694867
10	15.168126866036905	28.04764864343215	33.5158467109267	23.268377779604247
11	33.728342889966505	24.029152887353014	23.9937830322586	18.24872119042188
12	23.433221621232256	25.988254991720865	29.95392683888615	20.62459654816072
13	28.589924855219085	22.458934490041493	25.96655286131046	22.984587793428965
14	22.234155828684308	21.262177434771246	28.559080125188967	27.944586611355476
15	24.159827417271018	28.51863104808361	25.051646452902222	22.26989508174315
16	22.379606277179608	28.451400618556892	25.70437265605448	23.46462044820902
17	22.56569050176249	27.011487353198937	26.456005164183544	23.966816980855025
18	23.557062288808257	26.21968281643784	28.229115393459626	21.994139501294278
19	25.288892295559005	25.803463660013502	28.00322853820914	20.904415506218353
20	25.049799463080056	25.663092433529144	27.622933333825866	21.66417476956493
21	23.20687301852623	26.69805318037795	27.817513711590692	22.277560089505126
22	22.819189854854304	26.717631272492877	28.019297349661954	22.443881522990868
23	22.809585507779058	26.362916877146546	28.336610201109487	22.490887413964906
24	22.730903741354933	26.698976675289032	27.73384507264673	22.83627451070931
25	22.956882946096528	26.73296128801682	28.167425933399393	22.142729832487255
26	23.14712289777927	26.754478719445018	27.82056124479726	22.27783713797845
27	23.080815963163634	26.61604683227393	27.807817015024337	22.495320189538095
28	22.58009702237536	26.51723287678823	28.110538646876787	22.792131453959623
29	23.434237465634446	26.709227468802034	27.63484641817881	22.221688647384706
30	23.58412068970294	26.253482730183414	27.852421819229566	22.30997476088408
31	22.836459209691526	26.590743071710303	28.004059683629112	22.56873803496906
32	22.079655130060406	26.479923682380544	28.177861425894612	23.262559761664434
33	22.04391587700156	26.615862133291714	28.680704404978375	22.65951758472835
34	23.03759640132503	26.106277641357057	28.064086852849396	22.792039104468515
35	22.514528883688588	26.780151877973076	27.873200454728895	22.83211878360944
36	22.99566973236194	26.729729055828038	27.8516830233007	22.42291818850932
37	23.027068559338705	26.23704452076617	27.954283307921834	22.781603611973296
38	23.132531678184186	26.42543748262675	27.643157872378545	22.798872966810517
39	22.85188157470658	26.264287620643067	27.85011308195186	23.033717722698487
40	22.594134145023794	26.795389544005914	28.124391070543002	22.486085240427283
41	22.644834015642157	25.974956665001297	28.055590699667448	23.324618619689097
42	22.23212413987993	26.888016083587374	28.040168334652392	22.83969144188031
43	22.841261383229146	26.36771905068417	27.826748660701504	22.964270905385177
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	74.0
2	105.0
3	373.5
4	642.0
5	642.0
6	842.5
7	1043.0
8	977.5
9	912.0
10	1231.0
11	1550.0
12	1550.0
13	2508.5
14	3467.0
15	6097.5
16	8728.0
17	9948.5
18	11169.0
19	11169.0
20	10228.0
21	9287.0
22	9917.0
23	10547.0
24	13511.5
25	16476.0
26	16476.0
27	20457.5
28	24439.0
29	29002.0
30	33565.0
31	38665.0
32	43765.0
33	43765.0
34	50098.5
35	56432.0
36	60376.0
37	64320.0
38	67573.0
39	70826.0
40	70826.0
41	73364.0
42	75902.0
43	77378.0
44	78854.0
45	79415.5
46	79977.0
47	79977.0
48	79377.0
49	78777.0
50	79795.5
51	80814.0
52	77764.0
53	74714.0
54	74714.0
55	70156.5
56	65599.0
57	57762.0
58	49925.0
59	46501.5
60	43078.0
61	43078.0
62	38011.5
63	32945.0
64	27679.5
65	22414.0
66	19219.0
67	16024.0
68	16024.0
69	13423.0
70	10822.0
71	9177.0
72	7532.0
73	5679.0
74	3826.0
75	3826.0
76	2800.5
77	1775.0
78	1339.0
79	903.0
80	747.0
81	591.0
82	591.0
83	513.0
84	435.0
85	386.5
86	338.0
87	244.5
88	151.0
89	151.0
90	123.0
91	95.0
92	64.0
93	33.0
94	18.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1082843.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.16376010006812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.30092293015485	50.777499378240634
2	7.515927136571181	8.74309165802236
3	1.7966285417673273	3.134960144768701
4	0.8085684292156331	1.8811752056554796
5	0.47839128957302735	1.391251810034389
6	0.3126256756344345	1.0910090879233776
7	0.23461188109029754	0.9552136417853241
8	0.1732955000989719	0.8063614315342345
9	0.1377925291417118	0.7213078447722154
>10	1.0110358753961635	11.724332634701984
>50	0.13240358062111482	5.40573277474169
>100	0.09200604117019184	9.880287778196859
>500	0.0048254913042527926	1.8148974906135282
>1k	8.042485507087989E-4	1.0337028905504857
>5k	1.6084971014175976E-4	0.6391762284587672
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6832	0.630931723250739	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4765	0.44004532513023586	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2559	0.23632234774570274	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1446	0.1335373641423549	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1202	0.11100408831197135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.6174745554064626E-4	0.0	0.0
2	0.0	0.0	5.540969466487754E-4	0.0	0.0
3	0.0	0.0	8.311454199731633E-4	0.0	0.0
4	0.0	0.0	0.0012928928755138096	2.770484733243877E-4	0.0
5	9.234949110812925E-5	0.0	0.001846989822162585	2.770484733243877E-4	0.0
6	9.234949110812925E-5	0.0	0.002862834224352007	3.69397964432517E-4	0.0
7	9.234949110812925E-5	0.0	0.005818017939812143	5.540969466487754E-4	0.0
8	1.846989822162585E-4	0.0	0.006556813868677177	5.540969466487754E-4	0.0
9	2.770484733243877E-4	0.0	0.00858850267305602	0.0012005433844056801	0.0
10	8.311454199731633E-4	0.0	0.009604347075245441	0.0013852423666219388	0.0
11	8.311454199731633E-4	0.0	0.014129472139543775	0.0016622908399463265	0.0
12	9.234949110812925E-4	0.0	0.015145316541733197	0.0017546403310544558	0.0
13	9.234949110812925E-4	0.0	0.016253510435030746	0.0017546403310544558	0.0
14	9.234949110812925E-4	0.0	0.017546403310544557	0.001846989822162585	0.0
15	9.234949110812925E-4	0.0	0.019116344659382754	0.001846989822162585	0.0
16	0.0010158444021894216	0.0	0.02077863549932908	0.0020316888043788433	0.0
17	0.0010158444021894216	0.0	0.02207152837484289	0.0020316888043788433	0.0
18	0.0011081938932975509	0.0	0.022533275830383538	0.0022163877865951018	0.0
19	0.0011081938932975509	0.0	0.023087372777032312	0.0022163877865951018	0.0
20	0.0011081938932975509	0.0	0.023733819214789217	0.0022163877865951018	0.0
21	0.0011081938932975509	0.0	0.02456496463476238	0.002862834224352007	0.0
22	0.0011081938932975509	0.0	0.025211411072519285	0.003324581679892653	0.0
23	0.0011081938932975509	0.0	0.025396110054735542	0.004248076590973945	0.0
24	0.0011081938932975509	0.0	0.02567315852805993	0.0056333189575958845	0.0
25	0.0012005433844056801	0.0	0.02567315852805993	0.0060027169220284015	0.0
26	0.0012005433844056801	0.0	0.02567315852805993	0.006741512850893435	0.0
27	0.0012005433844056801	0.0	0.02567315852805993	0.007849706744190986	0.0
28	0.0012928928755138096	0.0	0.02576550801916806	0.018008150766085203	0.0
29	0.0012928928755138096	0.0	0.02576550801916806	0.04238841641863132	0.0
30	0.0012928928755138096	0.0	0.02576550801916806	0.08126755217515375	0.0
31	0.0012928928755138096	0.0	0.02576550801916806	0.1723241504077692	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1335	0.0	25.498127	1
ACGTCCG	40	0.0019311155	23.125	32
GTATCAA	3585	0.0	21.93166	1
GCCGCTC	385	0.0	21.142859	27
CGGATGC	70	5.101472E-6	21.142859	9
CCGCTCT	375	0.0	20.720001	28
GCCGGCA	405	0.0	20.555555	15
CGTCCGG	45	0.003825668	20.555555	33
TTCGCCG	380	0.0	20.447369	24
CCGGCAG	420	0.0	20.261904	16
TCTGTCG	65	6.9016576E-5	19.923077	8
AGCTTCG	425	0.0	19.588236	21
CGCCGGC	435	0.0	19.56322	14
TTGCGGT	125	4.129106E-10	19.240002	35
CGCTCTC	425	0.0	19.15294	29
CGAGTCG	155	1.8189894E-12	19.096773	21
CTCACGA	50	0.007034625	18.5	29
TACCCGA	70	1.2191145E-4	18.5	30
TTAACGG	220	0.0	18.5	35
ATACGTC	60	9.2363456E-4	18.5	29
>>END_MODULE