FastQCFastQC Report
Fri 10 Feb 2017
ERR1633309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736421
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48200.6545169135589561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.46209980432388537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19110.2594983032803247No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9600.13036021514867174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.1184105287600435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24950.022.244491
GGTATCA9400.021.6489351
CCGCTCT1201.0913936E-1121.58333228
GCCGCTC1301.8189894E-1221.34615327
GTCGCCA806.9531234E-720.812512
ACCCGGG450.003824523220.5555557
GCATAGA450.003824523220.5555551
TGCGGTA656.897828E-519.92307736
CGCTCTC1303.274181E-1119.92307729
TTCGCCG1303.274181E-1119.92307724
CCGGCAG1650.019.06060616
CGGCAGC1700.018.517
TAGCTCG701.21844234E-418.536
TCTATCG500.00703253918.531
GCTTCGC1950.018.02564222
GCCGGCA1552.0008883E-1117.90322715
TCGCCAT1054.791473E-717.61904713
CTTCGCC2050.017.14634123
GCTACAC2500.017.0225
TGCTCGC1757.2759576E-1216.91428610
Produced by FastQC (version 0.11.5)