##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633309.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736421
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.440431492312143	33.0	33.0	33.0	27.0	33.0
2	31.664264055479133	33.0	33.0	33.0	27.0	33.0
3	31.39423101731211	33.0	33.0	33.0	27.0	33.0
4	31.700280138670678	33.0	33.0	33.0	27.0	33.0
5	31.806253488154194	33.0	33.0	33.0	33.0	33.0
6	34.80264821345399	37.0	37.0	37.0	27.0	37.0
7	34.98352572781059	37.0	37.0	37.0	33.0	37.0
8	35.10890102264873	37.0	37.0	37.0	33.0	37.0
9	35.24016561179	37.0	37.0	37.0	33.0	37.0
10	35.24165253299403	37.0	37.0	37.0	33.0	37.0
11	35.285901678523565	37.0	37.0	37.0	33.0	37.0
12	35.28550380828358	37.0	37.0	37.0	33.0	37.0
13	35.272374090364075	37.0	37.0	37.0	33.0	37.0
14	35.27023265224647	37.0	37.0	37.0	33.0	37.0
15	35.274606509048496	37.0	37.0	37.0	33.0	37.0
16	35.25775745124053	37.0	37.0	37.0	33.0	37.0
17	35.23808663794215	37.0	37.0	37.0	33.0	37.0
18	35.22246921258356	37.0	37.0	37.0	33.0	37.0
19	35.20998586406417	37.0	37.0	37.0	33.0	37.0
20	35.20810378845796	37.0	37.0	37.0	33.0	37.0
21	35.20957984631074	37.0	37.0	37.0	33.0	37.0
22	35.11059434752676	37.0	37.0	37.0	33.0	37.0
23	35.16173628943227	37.0	37.0	37.0	33.0	37.0
24	35.13449507822292	37.0	37.0	37.0	33.0	37.0
25	35.15344755241907	37.0	37.0	37.0	33.0	37.0
26	35.03802444525618	37.0	37.0	37.0	27.0	37.0
27	35.049049388868596	37.0	37.0	37.0	27.0	37.0
28	35.068383438277834	37.0	37.0	37.0	33.0	37.0
29	35.06714365831501	37.0	37.0	37.0	33.0	37.0
30	35.07078559682573	37.0	37.0	37.0	33.0	37.0
31	35.053484352021464	37.0	37.0	37.0	27.0	37.0
32	35.045445472087295	37.0	37.0	37.0	27.0	37.0
33	35.02784955888004	37.0	37.0	37.0	27.0	37.0
34	34.99233183192766	37.0	37.0	37.0	27.0	37.0
35	34.92717752481257	37.0	37.0	37.0	27.0	37.0
36	34.944251997159235	37.0	37.0	37.0	27.0	37.0
37	34.93556403198714	37.0	37.0	37.0	27.0	37.0
38	34.90926114274308	37.0	37.0	37.0	27.0	37.0
39	34.79703865044587	37.0	37.0	37.0	27.0	37.0
40	34.585837448959225	37.0	37.0	37.0	27.0	37.0
41	34.745997194539534	37.0	37.0	37.0	27.0	37.0
42	34.782542866105125	37.0	37.0	37.0	27.0	37.0
43	34.37980720267347	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	33.0
16	42.0
17	51.0
18	33.0
19	44.0
20	89.0
21	279.0
22	647.0
23	1434.0
24	2601.0
25	4597.0
26	6896.0
27	10196.0
28	13739.0
29	17970.0
30	22713.0
31	28629.0
32	35802.0
33	46212.0
34	66853.0
35	130784.0
36	346773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32011444540555	20.854647002190323	14.076323190131731	23.748915362272395
2	15.819483692072877	22.628360679556938	39.072758652998765	22.479396975371422
3	18.09413365452642	27.8502378394967	30.75699905353052	23.29862945244636
4	12.595512621177289	18.24581319652753	39.58279299476794	29.57588118752724
5	13.857155078413028	37.39274138027026	34.93775978686105	13.812343754455672
6	28.580119252438486	40.45837910651652	17.325415760821596	13.636085880223407
7	25.531048136867362	33.18400751743907	23.511143761516852	17.773800584176712
8	24.378039192255514	35.589696654495185	20.850709037357706	19.181555115891587
9	26.207427544841877	15.40762688733754	20.445234451489025	37.93971111633155
10	14.688065658095029	28.392047483708367	33.96915622992826	22.95073062826834
11	32.92545975739421	24.30552632257907	24.247814768997625	18.5211991510291
12	22.682677435868882	26.595928144363075	30.519227452774977	20.202166966993065
13	28.222036036451975	22.832727475180636	26.547857815027005	22.397378673340384
14	21.983620782134132	21.836829748201097	28.764932015789878	27.41461745387489
15	23.75828500273621	28.853060952906013	25.48324939131285	21.90540465304493
16	22.114795748627483	29.13347120736644	25.665753692520987	23.08597935148509
17	21.97520168490578	27.725445091870004	26.94586384690279	23.353489376321424
18	22.956705471462655	26.890324963573825	28.495249320701067	21.657720244262453
19	24.432491740458243	26.295692273848793	28.839617555718807	20.432198429974157
20	24.399630102889518	26.009307176194053	28.565725312015815	21.025337408900615
21	22.661493900907224	27.316304124950264	28.038581192008376	21.983620782134132
22	22.121042175603357	27.337216076130368	28.215518025694543	22.326223722571736
23	21.98484290915115	26.86900563672139	28.933449752247697	22.212701701879766
24	22.54376233160108	27.36002911378138	28.157670680222317	21.938537874395216
25	22.542540204584064	26.93690158211132	28.652224746442588	21.868333466862026
26	22.50438268327492	27.0152535030913	28.322929411301416	22.157434402332363
27	22.49569200226501	26.978046525017618	28.17545941791448	22.35080205480289
28	21.757663075876437	27.233335279683768	28.68332108943118	22.325680555008617
29	22.927510214944984	26.99692159783602	28.0552835945743	22.020284592644696
30	22.369405543839733	27.275838141497864	28.484385969438677	21.870370345223726
31	22.56779749626912	26.93269203349714	28.53164154743007	21.967868922803667
32	21.52966849125704	26.9390742523638	28.637016054675247	22.894241201703917
33	21.687594460234024	26.895620847314238	29.220106433683995	22.196678258767744
34	22.07663822731834	26.69546360030472	28.52417299343718	22.703725178939766
35	22.08750157858073	27.51741191519525	28.32686737613403	22.06821913008999
36	22.662444444142686	27.29036787381131	28.095070618572798	21.952117063473203
37	22.846578248040185	26.788481045488926	27.794970539949293	22.569970166521596
38	22.50261738869478	27.242840712038358	28.14721470463227	22.10732719463459
39	22.25778460961868	26.903225193197912	28.200988293381098	22.63800190380231
40	22.377009889723407	27.20318947993064	28.43930306169976	21.980497568646197
41	22.217318626166282	26.87620260693272	28.47121415603303	22.435264610867968
42	22.058442113953838	27.08043361066564	28.366247024460193	22.49487725092033
43	22.152681686155066	26.82433010465481	28.325102081553894	22.69788612763623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	60.5
2	63.0
3	254.0
4	445.0
5	445.0
6	593.0
7	741.0
8	720.5
9	700.0
10	972.0
11	1244.0
12	1244.0
13	1921.0
14	2598.0
15	4768.0
16	6938.0
17	7810.5
18	8683.0
19	8683.0
20	8153.0
21	7623.0
22	8144.0
23	8665.0
24	10866.5
25	13068.0
26	13068.0
27	15935.5
28	18803.0
29	23242.5
30	27682.0
31	30212.5
32	32743.0
33	32743.0
34	36222.5
35	39702.0
36	42133.5
37	44565.0
38	46903.0
39	49241.0
40	49241.0
41	51127.5
42	53014.0
43	52130.5
44	51247.0
45	51588.5
46	51930.0
47	51930.0
48	51317.0
49	50704.0
50	51765.0
51	52826.0
52	52456.0
53	52086.0
54	52086.0
55	47716.5
56	43347.0
57	38668.5
58	33990.0
59	30219.5
60	26449.0
61	26449.0
62	22959.0
63	19469.0
64	16121.0
65	12773.0
66	10830.0
67	8887.0
68	8887.0
69	7368.5
70	5850.0
71	5054.0
72	4258.0
73	3430.0
74	2602.0
75	2602.0
76	1939.5
77	1277.0
78	1014.5
79	752.0
80	589.0
81	426.0
82	426.0
83	373.0
84	320.0
85	309.5
86	299.0
87	247.5
88	196.0
89	196.0
90	160.5
91	125.0
92	77.5
93	30.0
94	15.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	736421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.721680472569766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.08052868393519	55.872804564413684
2	6.501626680060148	8.155859023573344
3	1.4899371603112217	2.803540874797452
4	0.6763089875313578	1.6967694486667597
5	0.39084841440828455	1.225733468086348
6	0.27453943537080105	1.033174484546826
7	0.20065357294015268	0.8809730501342209
8	0.15088336652227186	0.7570926642908451
9	0.13133488248625785	0.7413790080785005
>10	0.9051423719610185	11.631687566143924
>50	0.12781936604280816	5.594599808083716
>100	0.06796540284576635	7.504911742094704
>500	0.0017539458797684755	0.7079171365932145
>1k	6.577297049131783E-4	1.3935571604964327
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4820	0.6545169135589561	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3403	0.46209980432388537	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1911	0.2594983032803247	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	960	0.13036021514867174	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	872	0.1184105287600435	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.431675631194657E-4	0.0	0.0
2	0.0	0.0	5.431675631194657E-4	0.0	0.0
3	1.3579189077986642E-4	0.0	6.78959453899332E-4	0.0	0.0
4	1.3579189077986642E-4	0.0	6.78959453899332E-4	0.0	0.0
5	1.3579189077986642E-4	0.0	9.505432354590649E-4	1.3579189077986642E-4	0.0
6	1.3579189077986642E-4	0.0	0.0017652945801382633	1.3579189077986642E-4	0.0
7	1.3579189077986642E-4	0.0	0.003937964832616126	4.073756723395992E-4	0.0
8	1.3579189077986642E-4	0.0	0.004888508068075191	4.073756723395992E-4	0.0
9	2.7158378155973283E-4	0.0	0.0051600918496349235	0.0014937107985785304	0.0
10	6.78959453899332E-4	0.0	0.005974843194314122	0.0014937107985785304	0.0
11	6.78959453899332E-4	0.0	0.009369640463810782	0.0017652945801382633	0.0
12	8.147513446791984E-4	0.0	0.009777016136150382	0.0019010864709181298	0.0
13	8.147513446791984E-4	0.0	0.010863351262389312	0.0021726702524778626	0.0
14	8.147513446791984E-4	0.0	0.011949686388628243	0.002308462143257729	0.0
15	8.147513446791984E-4	0.0	0.013171813405647041	0.002308462143257729	0.0
16	8.147513446791984E-4	0.0	0.014937107985785306	0.002308462143257729	0.0
17	8.147513446791984E-4	0.0	0.01548027554890477	0.0024442540340375955	0.0
18	8.147513446791984E-4	0.0	0.01588765122124437	0.0024442540340375955	0.0
19	8.147513446791984E-4	0.0	0.01588765122124437	0.0025800459248174618	0.0
20	8.147513446791984E-4	0.0	0.016295026893583968	0.002715837815597328	0.0
21	8.147513446791984E-4	0.0	0.016973986347483302	0.002715837815597328	0.0
22	8.147513446791984E-4	0.0	0.01710977823826317	0.003666381051056393	0.0
23	8.147513446791984E-4	0.0	0.01710977823826317	0.004752716177295324	0.0
24	9.505432354590649E-4	0.0	0.01710977823826317	0.005024299958855057	0.0
25	9.505432354590649E-4	0.0	0.01710977823826317	0.005567467521974523	0.0
26	9.505432354590649E-4	0.0	0.01710977823826317	0.006653802648213454	0.0
27	9.505432354590649E-4	0.0	0.01710977823826317	0.007468553992892653	0.0
28	9.505432354590649E-4	0.0	0.01710977823826317	0.014801316095005438	0.0
29	9.505432354590649E-4	0.0	0.01710977823826317	0.03367638891340687	0.0
30	9.505432354590649E-4	0.0	0.01710977823826317	0.06124214274171975	0.0
31	9.505432354590649E-4	0.0	0.01710977823826317	0.12587908275293616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2495	0.0	22.24449	1
GGTATCA	940	0.0	21.648935	1
CCGCTCT	120	1.0913936E-11	21.583332	28
GCCGCTC	130	1.8189894E-12	21.346153	27
GTCGCCA	80	6.9531234E-7	20.8125	12
ACCCGGG	45	0.0038245232	20.555555	7
GCATAGA	45	0.0038245232	20.555555	1
TGCGGTA	65	6.897828E-5	19.923077	36
CGCTCTC	130	3.274181E-11	19.923077	29
TTCGCCG	130	3.274181E-11	19.923077	24
CCGGCAG	165	0.0	19.060606	16
CGGCAGC	170	0.0	18.5	17
TAGCTCG	70	1.21844234E-4	18.5	36
TCTATCG	50	0.007032539	18.5	31
GCTTCGC	195	0.0	18.025642	22
GCCGGCA	155	2.0008883E-11	17.903227	15
TCGCCAT	105	4.791473E-7	17.619047	13
CTTCGCC	205	0.0	17.146341	23
GCTACAC	250	0.0	17.02	25
TGCTCGC	175	7.2759576E-12	16.914286	10
>>END_MODULE