FastQCFastQC Report
Fri 10 Feb 2017
ERR1633308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180827
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95310.8071461780599529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66920.566721458774232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37010.3134244050991381No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.142950660850404No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15480.13109456338650793No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14060.11906909310169907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC451.3232419E-424.6666688
CGGGTAG400.001931217823.12500217
GGTATCA21200.022.6886791
CTCGTAT502.7021626E-422.236
TCGTATG502.7021626E-422.237
GTATCAA49550.021.2441981
GCCGCTC2650.018.84905627
GAGTCGC2400.018.522
CCGCTCT2800.018.528
CGCTCTC2900.018.529
CCGTACC500.00703499318.59
TGTCGAC701.21923265E-418.536
CGCCCAT500.00703499318.535
CGAGTCG2600.017.78846221
CGCCGTA852.723217E-517.41176424
TTCGCCG2900.017.22413824
AAACCGT650.001579968617.0769236
CGCCGTC2500.017.0226
GCCGGCA3400.016.86764715
TCCGCCG2500.016.27999931
Produced by FastQC (version 0.11.5)