FastQCFastQC Report
Fri 10 Feb 2017
ERR1633307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595714
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160062.6868598018512238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124952.0974830203755497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69021.1586096683979226No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA31970.5366669240608748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29230.490671698163884No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25420.42671483295675444No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG24990.4194966040751098No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG22920.3847483859704489No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19770.33187066276770394No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18510.310719573486606No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18360.30820158666742764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.30719439193975634No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC16780.2816787921720826No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16410.27546775801810935No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16090.27009605280386223No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15470.2596883739512585No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14260.23937661360988663No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC13560.2276260084537211No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13070.21940058484440522No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12490.2096643691435823No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12420.20848930862796577No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12400.20815357705207532No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12110.2032854692016639No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC12010.20160681132221167No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11850.19892095871508814No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC11750.1972423008356359No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11690.19623510610796455No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11150.18717035355892256No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11090.18616315883125123No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10990.18448450095179902No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10790.18112718519289459No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10740.18028785625316845No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10630.17844133258577102No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10490.1760912115545379No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10300.1729017615835787No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10240.17189456685590737No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10100.16954444582467426No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10010.16803365373316725No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9910.16635499585371502No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT9820.16484420376220804No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT9430.15829743803234436No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.1577938406685087No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9060.15208640387837116No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9020.15141494072659029No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG9000.15107920915069983No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8870.14889695390741195No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8830.14822549075563107No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8640.14503604078467183No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8620.1447003092087814No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8570.14386098026905528No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8480.1423501881775483No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8460.14201445660165785No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8360.14033579872220564No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8260.1386571408427534No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC8220.13798567769097253No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC8070.13546769087179417No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8060.13529982508384894No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA8040.13496409350795852No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7930.13311756984056106No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7820.13127104617316362No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7790.13076744880932797No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7780.13059958302138275No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7650.12841732777809486No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7550.12673866989864263No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7480.1255636093830261No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7470.12539574359508088No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7450.1250600120191904No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7440.1248921462312452No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7250.12170269626028599No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7060.11851324628932676No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7000.11750605156165543No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC6960.11683458840987453No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG6910.11599525947014844No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.11599525947014844No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6810.1143166015906962No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6800.11414873580275098No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6750.11330940686302488No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6730.11297367528713444No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6710.11263794371124398No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6700.11247007792329876No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6670.1119664805594631No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.11011995689206566No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6550.10995209110412044No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.10827343322466822No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6430.10793770164877778No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6420.10776983586083255No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6420.10776983586083255No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6390.10726623849699687No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6340.10642690955727079No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6330.10625904376932556No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG6120.1027338622224759No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA6110.10256599643453067No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA6050.10155880170685933No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC6030.1012230701309689No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6010.10088733855507845No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5970.10021587540329756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGA200.001841224337.06
AATACTT250.005494244429.62
CAGAGAA250.005494244429.69
CGTATAG358.864362E-426.428571
GAGAACC358.864362E-426.4285719
TAACAAC358.864362E-426.428571
GCTGGTA601.3356002E-624.66666832
TATCTTC601.3356002E-624.66666837
ATAAACA601.3356002E-624.66666815
AATAAAC601.3356002E-624.66666814
GTATTGC451.3221672E-424.6666661
GCCGCTC5150.024.0679627
GCTCTCT4950.023.9191930
CGCTCTC5100.023.57843229
GCCGGCA5550.023.33333415
CCGGCAG5500.023.2090916
ACTAGTG1200.023.1250028
CTAGTGG802.7202987E-823.1259
CTAGTGC400.00193010123.1259
GGGATAG400.00193010123.12518
Produced by FastQC (version 0.11.5)