FastQCFastQC Report
Fri 10 Feb 2017
ERR1633306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489396
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136262.7842483387686046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112262.2938479268322585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65261.3334804534569142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.5488397943587605No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23410.47834473514291087No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG19510.3986546682032546No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17720.3620789708130021No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17250.3524752960792487No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17190.35124929504940783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.34777562546485874No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16940.3461409574250709No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14690.3001659188060385No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14200.2901535770623381No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG12860.26277288739589205No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12590.2572558827616082No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12420.25378221317705907No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11920.24356553792838517No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10610.21679784877685965No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9860.20147283590384882No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9460.19329949570490973No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9200.18798682457559932No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9000.18390015447612976No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8980.1834914874661828No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8950.18287848695126238No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8920.18226548643634194No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8900.18185681942639498No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8800.1798134843766602No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8620.17613548128713763No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8600.17572681427719067No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8410.1718444776826946No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8210.16775780758322503No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8090.1653058055235433No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7900.16142346892904724No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7880.16101480191910028No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7730.15794979934449813No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7640.15611079779973683No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.15427179625497553No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7350.15018512615550597No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7210.1473244570858773No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7210.1473244570858773No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7180.14671145657095686No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7120.14548545554111597No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7110.14528112203614252No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6990.14282911997646078No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6780.13853811637201777No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6710.1371077818372034No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6690.13669911482725644No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6690.13669911482725644No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6660.13608611431233603No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6580.1344514462725482No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6380.13036477617307865No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6260.1279127741133969No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG6180.1262781060736091No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6160.12586943906366216No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5960.12178276896419259No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5900.12055676793435173No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.1199437674194313No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.11933076690451086No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC5770.11790043236969652No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5730.11708309834980261No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5610.11463109629012089No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.11340509526028002No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5500.11238342773541264No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5430.11095309320059829No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5420.11074875969562482No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5400.11034009268567786No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5360.10952275866578395No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5350.10931842516081047No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5330.10890975815086351No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5290.1080924241309696No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5270.10768375712102264No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5260.10747942361604917No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5250.1072750901110757No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5220.10666208959615527No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5220.10666208959615527No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5210.10645775609118178No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5210.10645775609118178No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5200.10625342258620832No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5170.10564042207128786No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5170.10564042207128786No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5140.10502742155636745No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5130.10482308805139395No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.10400575403150004No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC5080.10380142052652658No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5030.10277975300165917No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5020.10257541949668572No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4940.10094075145689789No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4920.10053208444695094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTA200.001840903237.015
CGTTATT551.8511855E-830.27272810
GTTATTG551.8511855E-830.27272811
CGGGACC250.005493297729.616
AAGCTTC502.7153874E-729.636
TATCCCT250.005493297729.634
GTATCCC405.9332655E-527.75000233
GCATAAT358.862089E-426.4285711
AGGGTTG358.862089E-426.428576
CATAATG358.862089E-426.4285712
TTTTCAT358.862089E-426.4285735
TTATTGT659.368887E-825.61538512
CCGCTCT5150.024.78640728
CGCTCTC5100.024.66666629
GCTCTCT5200.024.19230830
GACCGTT1400.023.7857137
ATTGTTA701.9182335E-723.78571314
TTCGCTA701.9182335E-723.78571326
GCCGCTC5400.023.6388927
CCGGCAG5250.023.60952616
Produced by FastQC (version 0.11.5)