FastQCFastQC Report
Fri 10 Feb 2017
ERR1633305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018465
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49600.4870074082074494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35400.3475819002125748No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC20990.20609446569101542No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG18590.18252959110033234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18520.18184228225810412No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA17310.1699616579853014No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16730.16426681329255302No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15170.14894964480860903No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13930.1367744596034228No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13310.13068686700082968No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG13160.12921406233891197No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12800.12567933115030955No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA12700.12469746137569775No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12230.12008267343502231No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12150.11929717761533289No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT11770.11556607247180807No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG11520.11311139803527857No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11170.10967485382413729No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10870.10672924450030193No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC10800.10604193565807367No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10720.10525643983838424No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10660.10466731797361715No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT10580.10388182215392773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAG400.001931036823.12500221
GCCGTTC400.001931036823.1250028
CGTTAGA502.701809E-422.21
GCCGGCA2000.021.27500215
GTATCAA28800.021.2621521
GGTATCA12200.020.926231
TAACCGA450.003825513620.55555521
GTCTTAT759.264069E-619.7333341
CCGCTCT2200.018.528
TACACTC801.6163756E-518.55
GTATTAG7000.017.7071441
GCCGCTC2200.017.6590927
TTAACGG5250.017.61904735
AACGGCC5300.017.45283137
ACGGTAC852.7226224E-517.4117643
TTTAACG5350.017.2897234
TTCGCCG2250.017.26666624
CGCTCTC2300.016.89130429
TCTCGTC3750.016.77333312
AGCTTCG2650.016.75471721
Produced by FastQC (version 0.11.5)