##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633305.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018465
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.710269866907552	33.0	33.0	33.0	27.0	33.0
2	31.871888577417977	33.0	33.0	33.0	33.0	33.0
3	31.59160697716662	33.0	33.0	33.0	27.0	33.0
4	31.885207640910586	33.0	33.0	33.0	33.0	33.0
5	31.98707466628701	33.0	33.0	33.0	33.0	33.0
6	35.12246861698733	37.0	37.0	37.0	33.0	37.0
7	35.27470163432224	37.0	37.0	37.0	33.0	37.0
8	35.40057341194837	37.0	37.0	37.0	33.0	37.0
9	35.51948471474228	37.0	37.0	37.0	33.0	37.0
10	35.51839189368314	37.0	37.0	37.0	33.0	37.0
11	35.546596102958866	37.0	37.0	37.0	33.0	37.0
12	35.53037266867295	37.0	37.0	37.0	33.0	37.0
13	35.53981825590472	37.0	37.0	37.0	33.0	37.0
14	35.51319485696612	37.0	37.0	37.0	33.0	37.0
15	35.53163633507288	37.0	37.0	37.0	33.0	37.0
16	35.51903109090641	37.0	37.0	37.0	33.0	37.0
17	35.498618018292234	37.0	37.0	37.0	33.0	37.0
18	35.484266027796735	37.0	37.0	37.0	33.0	37.0
19	35.46984236080769	37.0	37.0	37.0	33.0	37.0
20	35.47219884826676	37.0	37.0	37.0	33.0	37.0
21	35.468379374843515	37.0	37.0	37.0	33.0	37.0
22	35.36879028734419	37.0	37.0	37.0	33.0	37.0
23	35.42533125831521	37.0	37.0	37.0	33.0	37.0
24	35.413042176216166	37.0	37.0	37.0	33.0	37.0
25	35.432991806296734	37.0	37.0	37.0	33.0	37.0
26	35.32172632343772	37.0	37.0	37.0	33.0	37.0
27	35.33058475254427	37.0	37.0	37.0	33.0	37.0
28	35.363071877776854	37.0	37.0	37.0	33.0	37.0
29	35.359559729593066	37.0	37.0	37.0	33.0	37.0
30	35.35224087229311	37.0	37.0	37.0	33.0	37.0
31	35.34168184473693	37.0	37.0	37.0	33.0	37.0
32	35.331425233071336	37.0	37.0	37.0	33.0	37.0
33	35.326116263200014	37.0	37.0	37.0	33.0	37.0
34	35.310117677092485	37.0	37.0	37.0	33.0	37.0
35	35.232243621528475	37.0	37.0	37.0	33.0	37.0
36	35.25822389576471	37.0	37.0	37.0	33.0	37.0
37	35.24891380656184	37.0	37.0	37.0	33.0	37.0
38	35.240338155950376	37.0	37.0	37.0	33.0	37.0
39	35.1316353532031	37.0	37.0	37.0	33.0	37.0
40	34.91848320757218	37.0	37.0	37.0	27.0	37.0
41	35.09910797130976	37.0	37.0	37.0	27.0	37.0
42	35.12568718610851	37.0	37.0	37.0	33.0	37.0
43	34.7748307501976	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	10.0
15	23.0
16	50.0
17	51.0
18	52.0
19	52.0
20	84.0
21	254.0
22	584.0
23	1435.0
24	2710.0
25	4819.0
26	7626.0
27	11146.0
28	15658.0
29	20542.0
30	26848.0
31	34109.0
32	43352.0
33	57350.0
34	82198.0
35	164793.0
36	544719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42881689601508	20.532271604817055	13.50090577486708	24.538005724300785
2	16.270269474159644	21.336521137201572	38.77619751292386	23.617011875714926
3	17.610325342549817	27.833357061852887	29.74623575675158	24.81008183884571
4	12.244210650341445	18.084666630664774	40.03348175931426	29.63764095967952
5	13.77651662060061	37.22435233414993	35.427825207542725	13.571305837706745
6	29.018768440741706	40.51106321768544	17.077955550755302	13.392212790817554
7	26.789040369575783	32.05628077548075	23.581959124761283	17.572719730182186
8	24.48527931740413	35.61114029446274	20.422400377037995	19.481180011095127
9	26.25568870800666	15.287417829773236	20.19470477630552	38.26218868591459
10	15.60495451488269	27.494121054724513	33.561585326938086	23.33933910345471
11	33.77308007638947	23.79315931328028	23.87170889524922	18.56205171508103
12	23.435464154389205	26.180673857226317	29.40965079801466	20.97421119036982
13	27.570117775279464	22.3678771484538	25.991467551658626	24.07053752460811
14	22.070861541633732	20.979415100175263	28.018144953434827	28.931578404756173
15	24.56058872911686	28.112502638775016	24.980632618695783	22.346276013412343
16	22.842611184478603	28.28855188936291	25.134982547264755	23.733854378893724
17	22.417854319981544	27.351160815541036	26.065795093596737	24.165189770880687
18	22.771425625819248	27.168042102575935	27.977495544765894	22.08303672683892
19	25.101893535860338	25.935599161483214	27.476152837849117	21.486354464807334
20	24.298920434182815	25.843303402669704	27.857118310398494	22.00065785274899
21	23.034075790527904	27.020467075451784	27.707579543725114	22.2378775902952
22	22.778986023083757	26.893511313594477	27.447678614385374	22.87982404893639
23	22.686101142405484	26.741910620394414	28.18496462814137	22.38702360905873
24	23.171832119905936	27.49873584266519	26.12009249213277	23.209339545296107
25	22.96141742720663	26.973730074180263	27.737919319760618	22.32693317885249
26	23.120971265581048	27.12375977574094	27.7913330354995	21.96393592317851
27	23.159165999813442	26.927778568728428	26.78609476025195	23.126960671206177
28	22.219320251555036	26.673474297103976	28.3257647538207	22.78144069752029
29	24.463089060497907	26.393641411339612	26.85433470958747	22.28893481857501
30	23.485244951962024	26.177531873947558	27.863991398820776	22.473231775269646
31	22.97378898636674	26.537681707275162	27.705321243243507	22.78320806311459
32	21.716701113931258	26.31597551216782	28.113680882504553	23.853642491396364
33	22.04710029308813	26.530415870943035	28.43121756761401	22.991266268354828
34	23.01895499599888	26.449117053605182	28.139798618509225	22.39212933188671
35	23.005012445199394	27.189545050639936	27.199167374431127	22.606275129729543
36	23.910198190414008	26.848247116984876	26.75752234981075	22.484032342790375
37	22.8140387740374	26.41337699380931	27.20427309725911	23.56831113489418
38	23.17458135527485	26.696940984717198	27.072506173506206	23.055971486501743
39	22.41697063718439	26.743285238078872	27.62107681658182	23.21866730815492
40	22.78468086777651	27.476840146691345	27.73890118953523	21.999577795996917
41	22.651441139361687	26.108899176702195	27.746854334709585	23.49280534922653
42	22.014404029593553	27.472814480615437	27.530253862430225	22.98252762736078
43	23.25195269351426	25.913212530622065	27.404476344302452	23.43035843156122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	43.5
2	65.0
3	268.5
4	472.0
5	472.0
6	595.0
7	718.0
8	687.5
9	657.0
10	922.0
11	1187.0
12	1187.0
13	1903.5
14	2620.0
15	4619.5
16	6619.0
17	7304.5
18	7990.0
19	7990.0
20	6929.0
21	5868.0
22	6013.0
23	6158.0
24	8240.0
25	10322.0
26	10322.0
27	13632.0
28	16942.0
29	20641.0
30	24340.0
31	30277.0
32	36214.0
33	36214.0
34	45824.5
35	55435.0
36	60627.5
37	65820.0
38	70877.0
39	75934.0
40	75934.0
41	79454.0
42	82974.0
43	85674.5
44	88375.0
45	85649.0
46	82923.0
47	82923.0
48	79495.0
49	76067.0
50	76572.0
51	77077.0
52	73031.0
53	68985.0
54	68985.0
55	67282.5
56	65580.0
57	55028.0
58	44476.0
59	40367.5
60	36259.0
61	36259.0
62	31902.0
63	27545.0
64	22093.0
65	16641.0
66	13917.0
67	11193.0
68	11193.0
69	9302.0
70	7411.0
71	6274.5
72	5138.0
73	4857.5
74	4577.0
75	4577.0
76	3822.0
77	3067.0
78	2279.0
79	1491.0
80	1030.5
81	570.0
82	570.0
83	434.0
84	298.0
85	257.5
86	217.0
87	179.0
88	141.0
89	141.0
90	101.5
91	62.0
92	37.0
93	12.0
94	7.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1018465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.46015509738322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.05556229256169	40.36750178300118
2	8.48411650282578	8.0531497017676
3	2.201696702914849	3.13478600993208
4	0.9642314816025498	1.8305030266654654
5	0.5615494478248033	1.332561194430754
6	0.3906842899665359	1.11251621975537
7	0.27778131470386186	0.9228480995300212
8	0.21353327734562497	0.8107457969020727
9	0.1692676210464953	0.723012079304858
>10	1.3234298506857392	12.758440233870871
>50	0.18223882490098675	6.1113971121543385
>100	0.15798008013211887	14.792908534666365
>500	0.013077122780179744	4.338990230019846
>1k	0.004851190708776356	3.710639977999207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4960	0.4870074082074494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3540	0.3475819002125748	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2099	0.20609446569101542	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1859	0.18252959110033234	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1852	0.18184228225810412	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1731	0.1699616579853014	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1673	0.16426681329255302	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1517	0.14894964480860903	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1393	0.1367744596034228	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1331	0.13068686700082968	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1316	0.12921406233891197	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1280	0.12567933115030955	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1270	0.12469746137569775	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1223	0.12008267343502231	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1215	0.11929717761533289	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1177	0.11556607247180807	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1152	0.11311139803527857	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1117	0.10967485382413729	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1087	0.10672924450030193	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	1080	0.10604193565807367	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1072	0.10525643983838424	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	1066	0.10466731797361715	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	1058	0.10388182215392773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.9456093238353797E-4	0.0	0.0
2	0.0	0.0	3.927479098447173E-4	9.818697746117932E-5	0.0
3	0.0	0.0	7.854958196894346E-4	1.9637395492235865E-4	0.0
4	0.0	0.0	8.83682797150614E-4	3.927479098447173E-4	0.0
5	9.818697746117932E-5	0.0	8.83682797150614E-4	3.927479098447173E-4	0.0
6	1.9637395492235865E-4	0.0	0.001865552571762407	3.927479098447173E-4	0.0
7	2.9456093238353797E-4	0.0	0.004123853053369531	5.891218647670759E-4	0.0
8	3.927479098447173E-4	0.0	0.005105722827981325	5.891218647670759E-4	0.0
9	3.927479098447173E-4	0.0	0.006578527489899014	0.0013746176844565106	0.0
10	7.854958196894346E-4	0.0	0.007364023309588449	0.0013746176844565106	0.0
11	7.854958196894346E-4	0.0	0.009818697746117933	0.0014728046619176898	0.0
12	7.854958196894346E-4	0.0	0.010702380543268546	0.0014728046619176898	0.0
13	7.854958196894346E-4	0.0	0.011880624272802698	0.0014728046619176898	0.0
14	7.854958196894346E-4	0.0	0.012960681024875671	0.0014728046619176898	0.0
15	8.83682797150614E-4	0.0	0.014728046619176899	0.0015709916393788692	0.0
16	8.83682797150614E-4	0.0	0.015218981506482794	0.0015709916393788692	0.0
17	8.83682797150614E-4	0.0	0.01580810337124987	0.0015709916393788692	0.0
18	8.83682797150614E-4	0.0	0.01610266430363341	0.0016691786168400486	0.0
19	9.818697746117933E-4	0.0	0.01629903825855577	0.0016691786168400486	0.0
20	9.818697746117933E-4	0.0	0.016986347100784022	0.002160113504145945	0.0
21	9.818697746117933E-4	0.0	0.017575468965551098	0.002651048391451842	0.0
22	9.818697746117933E-4	0.0	0.01767365594301228	0.0032401702562189177	0.0
23	9.818697746117933E-4	0.0	0.017771842920473457	0.003632918166063635	0.0
24	9.818697746117933E-4	0.0	0.017771842920473457	0.00432022700829189	0.0
25	0.0010800567520729725	0.0	0.017771842920473457	0.004909348873058966	0.0
26	0.0010800567520729725	0.0	0.017771842920473457	0.00579303167020958	0.0
27	0.0010800567520729725	0.0	0.017771842920473457	0.007462210287049628	0.0
28	0.0010800567520729725	9.818697746117932E-5	0.017771842920473457	0.015709916393788693	0.0
29	0.0010800567520729725	9.818697746117932E-5	0.017870029897934635	0.03731105143524814	0.0
30	0.0010800567520729725	9.818697746117932E-5	0.017870029897934635	0.06686533165106312	0.0
31	0.0010800567520729725	9.818697746117932E-5	0.017870029897934635	0.13805089031041812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAG	40	0.0019310368	23.125002	21
GCCGTTC	40	0.0019310368	23.125002	8
CGTTAGA	50	2.701809E-4	22.2	1
GCCGGCA	200	0.0	21.275002	15
GTATCAA	2880	0.0	21.262152	1
GGTATCA	1220	0.0	20.92623	1
TAACCGA	45	0.0038255136	20.555555	21
GTCTTAT	75	9.264069E-6	19.733334	1
CCGCTCT	220	0.0	18.5	28
TACACTC	80	1.6163756E-5	18.5	5
GTATTAG	700	0.0	17.707144	1
GCCGCTC	220	0.0	17.65909	27
TTAACGG	525	0.0	17.619047	35
AACGGCC	530	0.0	17.452831	37
ACGGTAC	85	2.7226224E-5	17.411764	3
TTTAACG	535	0.0	17.28972	34
TTCGCCG	225	0.0	17.266666	24
CGCTCTC	230	0.0	16.891304	29
TCTCGTC	375	0.0	16.773333	12
AGCTTCG	265	0.0	16.754717	21
>>END_MODULE