FastQCFastQC Report
Fri 10 Feb 2017
ERR1633304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939531
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT231092.459631454417151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT174561.8579482741921234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94401.0047566285731924No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA54170.5765642645106973No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA45830.4877965708422607No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG42460.451927610690866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42090.4479894756000601No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA35040.3729520367076765No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG31100.33101621979477No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT30340.32292707744608745No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT27000.28737742554529866No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC26580.28290711003681623No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT24190.25746888607188056No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24120.25672383348713346No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA24080.2562980891529923No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC23970.255127292234104No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC23010.24490942821471565No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT22610.2406519848733038No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG21880.2328821507752272No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC20400.21712961041200343No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA20260.2156395052425093No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC20090.21383009182240928No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC19630.20893403197978563No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19230.20467658863837385No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG18990.20212212263352672No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18670.1987161679603973No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA18160.19328792770009717No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA18020.19179782253060304No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17330.18445373276666763No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA17100.18200570284535583No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG16910.1799834172581852No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA16890.1797705450911146No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC16760.17838687600515576No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG16550.17615171825091455No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT16250.17295863574485568No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC16190.1723200192436439No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC16090.17125565840829096No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT15850.16870119240344383No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG15710.1672110872339497No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA15630.16635959856566734No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15430.1642308768949614No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG15040.1600798696370849No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT15030.1599734335535496No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA15030.1599734335535496No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG14920.15880263663466135No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG14880.15837689230052016No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14830.15784471188284366No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC14800.1575254036322378No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.15624817062981422No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA14590.15529024587799659No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14590.15529024587799659No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG14350.15273577987314949No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT14130.15039418603537297No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT13810.14698823136224348No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG13710.14592387052689054No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA13500.14368871277264933No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG13460.14326296843850816No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13440.14305009627143755No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13090.1393248333477022No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12640.1345352095886139No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT12350.1314485631660903No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12320.13112925491548444No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12280.13070351058134325No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12130.1291069693283138No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG11930.12697824765760787No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG11830.12591388682225493No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.12591388682225493No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC11500.12240149606559017No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC11440.12176287956437841No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.12037921047841957No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11280.12005990222781367No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT11270.11995346614427838No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11090.11803761664064304No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10940.1164410753876136No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA10930.11633463930407831No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA10870.11569602280286655No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG10840.11537671455226066No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC10830.11527027846872535No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG10710.11399304546630182No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10690.11378017329923122No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG10530.11207719596266648No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC10510.11186432379559588No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA10370.11037421862610175No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG10340.11005491037549585No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT10320.10984203820842527No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10290.10952272995781939No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10260.1092034217072135No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT10210.10867124128953701No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA10130.10781975262125465No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA9990.10632964745176052No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC9980.10622321136822521No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9900.10537172269994284No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG9850.10483954228226637No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9610.10228507627741926No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG9550.1016464597762075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCATA200.00184176537.07
TGTCAAC502.7198803E-729.62
CAGCACA509.088779E-625.90000226
TGCCTCA601.3369718E-624.66666819
AACAACG701.9221443E-723.7857152
GTTATCG551.9021802E-523.54545411
TCAGCAC952.8194336E-1023.3684223
ACCGTAG952.8194336E-1023.3684228
GATCAAT400.001930926123.12511
GACCCAA400.001930926123.12535
CTAACCG400.001930926123.12531
TAACAAC802.7244823E-823.1251
CATACCC400.001930926123.1253
ATGAACT400.001930926123.1254
GCCGGCA8650.022.88439415
GGCAGCT8500.022.85294218
GCCTCAC652.6819798E-622.7692320
CATTTTG652.6819798E-622.7692336
CTAGTGC903.8253347E-922.611119
ACTAGTG1650.022.4242428