FastQCFastQC Report
Fri 10 Feb 2017
ERR1633303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120967
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56950.5080435017266343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.32079445692870534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21370.19063897509917777No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15360.13702455112416334No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14160.12631950806758807No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13560.12096698653930045No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13230.11802309969874225No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12510.11160007386479709No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12100.10794251748713388No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11880.10597992626009507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.026.3179911
GTATCAA30500.022.6852461
GCCGGCA2300.022.5217415
TTCGCCG2550.021.03921524
AGCTTCG2700.020.55555521
AGCGTGA1254.129106E-1019.24000216
GCTTCGC2950.018.81355922
GTCGATT1306.9667294E-1018.512
GGTCGAT1205.173206E-918.511
CGATTGG1205.173206E-918.514
GCCGCTC2900.018.527
CGTTATT500.00703477618.510
CCGGCAG2900.017.86206816
GACCGTT953.6062265E-617.5263167
TGCTCGC3200.017.3437510
AACTCGC1603.45608E-1117.3437522
CCGCTCT3100.017.30645228
CGCCGTT650.001579900617.07692325
ATCGCTA650.001579900617.07692310
CGCCGGC3150.017.03174614
Produced by FastQC (version 0.11.5)