FastQCFastQC Report
Fri 10 Feb 2017
ERR1633302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128140
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70990.6292658712571134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.43948446114843903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27650.24509369404506534No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG18750.16620277625117452No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA17270.15308383711241513No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG15970.14156044462566703No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG14440.1279982980835712No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12790.11337245377346784No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.10397645682273476No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC11380.10087400499937951No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT11370.10078536351871223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38100.023.646981
GGTATCA13050.023.107281
CCGCTCT3500.021.67142928
CGCTCTC3350.021.53731329
TTCGCCG3500.021.14285724
GCCGCTC3550.020.8450727
TGCTCGC4200.018.94047510
CGAGTCG2150.018.93023321
TTAACGG3050.018.8032835
GCCGGCA3850.018.7402615
TTTAACG3250.018.21538434
GGACCGT1750.017.9714286
GACGTAT953.6062684E-617.52631628
GGACGTA953.6062684E-617.52631627
CGAACTA3300.017.37878829
GCACCGC3300.017.37878810
CCGGCAG3750.017.26666816
GACCGTT1502.5102054E-1017.2666667
CGCCGTT1502.5102054E-1017.26666625
TATTAGA4400.017.2386362
Produced by FastQC (version 0.11.5)