##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633302.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1128140
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731820518729943	33.0	33.0	33.0	27.0	33.0
2	31.878652472210895	33.0	33.0	33.0	33.0	33.0
3	31.600580601698372	33.0	33.0	33.0	27.0	33.0
4	31.89391476235219	33.0	33.0	33.0	33.0	33.0
5	31.995548424840887	33.0	33.0	33.0	33.0	33.0
6	35.1444067225699	37.0	37.0	37.0	33.0	37.0
7	35.29179800379386	37.0	37.0	37.0	33.0	37.0
8	35.426484301593774	37.0	37.0	37.0	33.0	37.0
9	35.54898239580194	37.0	37.0	37.0	33.0	37.0
10	35.55242257166664	37.0	37.0	37.0	33.0	37.0
11	35.57495612246707	37.0	37.0	37.0	33.0	37.0
12	35.5444342014289	37.0	37.0	37.0	33.0	37.0
13	35.552812594181574	37.0	37.0	37.0	33.0	37.0
14	35.534857375857605	37.0	37.0	37.0	33.0	37.0
15	35.56188770897229	37.0	37.0	37.0	33.0	37.0
16	35.54980676157214	37.0	37.0	37.0	33.0	37.0
17	35.52345276295495	37.0	37.0	37.0	33.0	37.0
18	35.51411881504069	37.0	37.0	37.0	33.0	37.0
19	35.503905543638204	37.0	37.0	37.0	33.0	37.0
20	35.49480029074406	37.0	37.0	37.0	33.0	37.0
21	35.49217473008669	37.0	37.0	37.0	33.0	37.0
22	35.38841012640275	37.0	37.0	37.0	33.0	37.0
23	35.44917297498537	37.0	37.0	37.0	33.0	37.0
24	35.43476252947329	37.0	37.0	37.0	33.0	37.0
25	35.459442976935485	37.0	37.0	37.0	33.0	37.0
26	35.35173471377666	37.0	37.0	37.0	33.0	37.0
27	35.35466520112752	37.0	37.0	37.0	33.0	37.0
28	35.38536883720106	37.0	37.0	37.0	33.0	37.0
29	35.38836757849203	37.0	37.0	37.0	33.0	37.0
30	35.37888737213466	37.0	37.0	37.0	33.0	37.0
31	35.371281933093414	37.0	37.0	37.0	33.0	37.0
32	35.35817806300636	37.0	37.0	37.0	33.0	37.0
33	35.35606485010726	37.0	37.0	37.0	33.0	37.0
34	35.33075948020636	37.0	37.0	37.0	33.0	37.0
35	35.25625542929069	37.0	37.0	37.0	33.0	37.0
36	35.292260712322935	37.0	37.0	37.0	33.0	37.0
37	35.271039941851186	37.0	37.0	37.0	33.0	37.0
38	35.261250376726295	37.0	37.0	37.0	33.0	37.0
39	35.166000673675256	37.0	37.0	37.0	33.0	37.0
40	34.93548407112593	37.0	37.0	37.0	27.0	37.0
41	35.09953285939688	37.0	37.0	37.0	27.0	37.0
42	35.13871682592586	37.0	37.0	37.0	33.0	37.0
43	34.788151293279206	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	40.0
16	47.0
17	44.0
18	38.0
19	56.0
20	92.0
21	252.0
22	683.0
23	1441.0
24	3063.0
25	5176.0
26	8228.0
27	12391.0
28	17222.0
29	22841.0
30	29712.0
31	37384.0
32	47534.0
33	61799.0
34	88824.0
35	178034.0
36	613234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87570691580832	20.113106529331464	13.462513517825803	23.54867303703441
2	16.5800343928945	21.934777598525006	38.74421614338646	22.740971865194037
3	18.15244561845161	27.311415249880334	29.80312727143794	24.733011860230114
4	12.19431985391884	17.994486499902496	39.625046536777354	30.18614710940132
5	14.436594748878687	36.319783005655324	34.725654617334726	14.51796762813126
6	29.268619143014163	40.1693938695552	17.03192866133636	13.530058326094279
7	27.64816423493538	31.90224617512011	22.64851880085805	17.801070789086463
8	24.91162444377471	34.910117538603366	20.38337440388604	19.794883613735884
9	26.569131490772424	15.025617387912849	20.133671352846278	38.27157976846845
10	15.72526459481979	27.435779247256548	33.18506568333717	23.653890474586486
11	34.22456432712252	24.090892974276244	23.218838087471415	18.465704611129823
12	23.7126597762689	25.622972326129734	29.602177034765187	21.06219086283617
13	28.50718882408212	22.311060683957663	25.86771145425213	23.31403903770809
14	22.777048947825623	21.090910702572376	27.777048947825623	28.354991401776374
15	24.323310936585884	28.20944209052068	25.256262520609145	22.21098445228429
16	23.411810590884112	28.227347669615472	24.87687698335313	23.483964756147287
17	23.178860779690464	26.624532416189478	25.666495293137377	24.53011151098268
18	23.510557200347474	25.900065594695693	28.10936585884731	22.480011346109524
19	26.25764532770755	25.46926799865265	27.374173418192775	20.89891325544702
20	25.346322264967114	25.559859591894625	27.297321254454232	21.79649688868403
21	23.17380821529243	26.791267041324655	27.356799687981987	22.678125055400926
22	23.787295902990763	25.866825039445455	27.40794582232702	22.937933235236763
23	22.65179853564274	26.234598542734055	28.36571702093712	22.747885900686086
24	23.094917297498537	27.12642047972769	26.67310794759516	23.105554275178612
25	23.16184161540234	26.642083429361602	28.04696225645753	22.14911269877852
26	23.97539312496676	26.34185473434148	27.056659634442532	22.626092506249222
27	23.645114968000424	26.251263141099507	26.746503093587677	23.35711879731239
28	22.835906890988706	26.01255163366249	27.95716843654156	23.19437303880724
29	24.378268654599605	26.476589784955767	27.121545198290992	22.023596362153633
30	24.122626624355135	25.847501196659987	27.241565763114505	22.78830641587037
31	23.213874164554042	25.777297143971495	27.691243994539683	23.31758469693478
32	22.301221479603594	26.098888435832436	27.727321077171275	23.8725690073927
33	22.677504565036255	25.78102008615952	28.263336110766396	23.27813923803783
34	23.53227436311096	25.806282908149697	27.961777793536264	22.699664935203078
35	23.348431932206996	26.792596663534667	26.816263938872837	23.0427074653855
36	23.626943464463633	26.19320297126243	26.75518995869307	23.424663605580868
37	23.49921109082206	26.021149857287217	27.371248249330755	23.108390802559967
38	23.370503660893153	25.862924814296097	26.842413175669687	23.924158349141063
39	22.91896395837396	25.990391263495667	27.57326218377152	23.517382594358857
40	23.550091300725086	26.72478593082419	27.261244171822646	22.463878596628078
41	23.274682220291808	25.817717659155782	27.42709238215115	23.48050773840126
42	22.40821174676902	27.504565036254363	26.979984753665327	23.10723846331129
43	23.905898204123602	25.5566684985906	26.846047476377045	23.691385820908753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	46.0
2	71.0
3	353.5
4	636.0
5	636.0
6	796.5
7	957.0
8	927.0
9	897.0
10	1242.5
11	1588.0
12	1588.0
13	2499.5
14	3411.0
15	6113.0
16	8815.0
17	9872.5
18	10930.0
19	10930.0
20	9549.5
21	8169.0
22	8335.5
23	8502.0
24	10809.0
25	13116.0
26	13116.0
27	16263.5
28	19411.0
29	23261.5
30	27112.0
31	32425.0
32	37738.0
33	37738.0
34	48544.0
35	59350.0
36	63897.5
37	68445.0
38	71550.0
39	74655.0
40	74655.0
41	76032.5
42	77410.0
43	81225.5
44	85041.0
45	84600.0
46	84159.0
47	84159.0
48	81804.0
49	79449.0
50	82843.0
51	86237.0
52	85886.5
53	85536.0
54	85536.0
55	81768.5
56	78001.0
57	66315.5
58	54630.0
59	50482.0
60	46334.0
61	46334.0
62	41544.0
63	36754.0
64	28731.5
65	20709.0
66	18083.5
67	15458.0
68	15458.0
69	13233.0
70	11008.0
71	10836.5
72	10665.0
73	9448.5
74	8232.0
75	8232.0
76	5093.0
77	1954.0
78	1514.5
79	1075.0
80	867.0
81	659.0
82	659.0
83	530.5
84	402.0
85	360.0
86	318.0
87	240.0
88	162.0
89	162.0
90	130.5
91	99.0
92	60.5
93	22.0
94	12.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1128140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.88884361199265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.93176025801432	42.870361488153215
2	8.202278341802431	8.184043629124318
3	1.9917802746545317	2.981028438950751
4	0.9120623263795307	1.8200693906055436
5	0.5358811139662302	1.336724454464083
6	0.36692793237739724	1.0983366141148672
7	0.26358517147108884	0.9204971578572991
8	0.20536951159492622	0.8196517957304467
9	0.1535974449093957	0.6896519017457848
>10	1.1164009364678944	11.215042635935877
>50	0.15937007233414138	5.651832365717242
>100	0.1452497222255099	14.966626262176682
>500	0.011757449392739225	4.013732724947541
>1k	0.0037985605730388263	2.791781314620758
>5k	1.8088383681137267E-4	0.6406198258555375
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7099	0.6292658712571134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4958	0.43948446114843903	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2765	0.24509369404506534	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1875	0.16620277625117452	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1727	0.15308383711241513	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1597	0.14156044462566703	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1444	0.1279982980835712	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1279	0.11337245377346784	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1173	0.10397645682273476	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1138	0.10087400499937951	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1137	0.10078536351871223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.318488840037583E-4	0.0	0.0
2	0.0	0.0	6.204903646710515E-4	0.0	0.0
3	0.0	0.0	8.864148066729307E-4	0.0	0.0
4	0.0	0.0	0.00115233924867481	8.864148066729307E-5	0.0
5	8.864148066729307E-5	0.0	0.0014182636906766891	8.864148066729307E-5	0.0
6	8.864148066729307E-5	0.0	0.002570602939351499	8.864148066729307E-5	0.0
7	8.864148066729307E-5	0.0	0.005318488840037584	8.864148066729307E-5	0.0
8	8.864148066729307E-5	0.0	0.0062049036467105145	8.864148066729307E-5	0.0
9	8.864148066729307E-5	0.0	0.007889091779389084	7.091318453383446E-4	0.0
10	4.4320740333646534E-4	0.0	0.008775506586062014	8.864148066729307E-4	0.0
11	4.4320740333646534E-4	0.0	0.012321165812753736	8.864148066729307E-4	0.0
12	4.4320740333646534E-4	0.0	0.014005353945432305	8.864148066729307E-4	0.0
13	4.4320740333646534E-4	0.0	0.015334976155441701	8.864148066729307E-4	0.0
14	4.4320740333646534E-4	0.0	0.016310032442781924	8.864148066729307E-4	0.0
15	4.4320740333646534E-4	0.0	0.0179055790947932	9.750562873402237E-4	0.0
16	4.4320740333646534E-4	0.0	0.019412484266137182	0.0010636977680075167	0.0
17	5.318488840037583E-4	0.0	0.01994433315014094	0.00115233924867481	0.0
18	5.318488840037583E-4	0.0	0.02029889907281011	0.001240980729342103	0.0
19	5.318488840037583E-4	0.0	0.021008030918148457	0.001240980729342103	0.0
20	5.318488840037583E-4	0.0	0.02136259684081763	0.0014182636906766891	0.0
21	5.318488840037583E-4	0.0	0.02224901164749056	0.0021273955360150334	0.0
22	5.318488840037583E-4	0.0	0.022780860531494317	0.0031024518233552572	0.0
23	5.318488840037583E-4	0.0	0.022958143492828903	0.003722942188026309	0.0
24	5.318488840037583E-4	0.0	0.023046784973496198	0.004875281436701118	0.0
25	5.318488840037583E-4	0.0	0.023046784973496198	0.005584413282039463	0.0
26	5.318488840037583E-4	0.0	0.023046784973496198	0.0062049036467105145	0.0
27	5.318488840037583E-4	0.0	0.023046784973496198	0.007357242895385325	0.0
28	6.204903646710515E-4	0.0	0.023046784973496198	0.01852606945946425	0.0
29	6.204903646710515E-4	0.0	0.023046784973496198	0.04361160848830819	0.0
30	6.204903646710515E-4	0.0	0.023046784973496198	0.0728632971085149	0.0
31	6.204903646710515E-4	0.0	0.023046784973496198	0.15015866825039445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3810	0.0	23.64698	1
GGTATCA	1305	0.0	23.10728	1
CCGCTCT	350	0.0	21.671429	28
CGCTCTC	335	0.0	21.537313	29
TTCGCCG	350	0.0	21.142857	24
GCCGCTC	355	0.0	20.84507	27
TGCTCGC	420	0.0	18.940475	10
CGAGTCG	215	0.0	18.930233	21
TTAACGG	305	0.0	18.80328	35
GCCGGCA	385	0.0	18.74026	15
TTTAACG	325	0.0	18.215384	34
GGACCGT	175	0.0	17.971428	6
GACGTAT	95	3.6062684E-6	17.526316	28
GGACGTA	95	3.6062684E-6	17.526316	27
CGAACTA	330	0.0	17.378788	29
GCACCGC	330	0.0	17.378788	10
CCGGCAG	375	0.0	17.266668	16
GACCGTT	150	2.5102054E-10	17.266666	7
CGCCGTT	150	2.5102054E-10	17.266666	25
TATTAGA	440	0.0	17.238636	2
>>END_MODULE