FastQCFastQC Report
Fri 10 Feb 2017
ERR1633301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084920
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36060.3323747373078199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.23273605427128266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13990.12894959997050473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21550.024.4663581
GGTATCA6750.023.0222231
TTCGCCG2050.020.75609624
TACGACT450.003825671320.5555574
CGGCGTA656.901672E-519.92307712
GCGAACT2250.018.08888828
CGAACTA1950.018.02564229
GCCGGCA2700.017.81481615
TTAACGG2300.017.69565235
AGCTTCG2750.017.4909121
TAACGGC2450.017.36734636
TGCTCGC2950.016.30508410
GGTTAAC803.382347E-416.18751
TCAACGC32050.016.1622474
ATCAACG31850.016.1475683
TGCGGTA1504.669346E-916.03333336
AACGCAG32850.015.7686466
CAACGCA32800.015.6798775
TATCAAC33150.015.5701362
CCGCTCT2750.015.47272828
Produced by FastQC (version 0.11.5)