##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084920
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.781047450503262	33.0	33.0	33.0	27.0	33.0
2	31.92348007226339	33.0	33.0	33.0	33.0	33.0
3	31.649835932603324	33.0	33.0	33.0	27.0	33.0
4	31.934836669984882	33.0	33.0	33.0	33.0	33.0
5	32.02835139918151	33.0	33.0	33.0	33.0	33.0
6	35.19638867381927	37.0	37.0	37.0	33.0	37.0
7	35.33806916639015	37.0	37.0	37.0	33.0	37.0
8	35.463956789440694	37.0	37.0	37.0	33.0	37.0
9	35.59067396674409	37.0	37.0	37.0	33.0	37.0
10	35.59705231722155	37.0	37.0	37.0	33.0	37.0
11	35.62705452936622	37.0	37.0	37.0	33.0	37.0
12	35.61004498027504	37.0	37.0	37.0	33.0	37.0
13	35.60787615676732	37.0	37.0	37.0	33.0	37.0
14	35.58276831471445	37.0	37.0	37.0	33.0	37.0
15	35.604579139475725	37.0	37.0	37.0	33.0	37.0
16	35.586921616340376	37.0	37.0	37.0	33.0	37.0
17	35.57621207093611	37.0	37.0	37.0	33.0	37.0
18	35.55941820595067	37.0	37.0	37.0	33.0	37.0
19	35.54560336245991	37.0	37.0	37.0	33.0	37.0
20	35.55508240238912	37.0	37.0	37.0	33.0	37.0
21	35.55194484385945	37.0	37.0	37.0	33.0	37.0
22	35.468947940862	37.0	37.0	37.0	33.0	37.0
23	35.50770840246285	37.0	37.0	37.0	33.0	37.0
24	35.49793072300262	37.0	37.0	37.0	33.0	37.0
25	35.51917929432585	37.0	37.0	37.0	33.0	37.0
26	35.40461877373447	37.0	37.0	37.0	33.0	37.0
27	35.42305515614054	37.0	37.0	37.0	33.0	37.0
28	35.445981270508426	37.0	37.0	37.0	33.0	37.0
29	35.45337167717435	37.0	37.0	37.0	33.0	37.0
30	35.45594421708513	37.0	37.0	37.0	33.0	37.0
31	35.442334918703686	37.0	37.0	37.0	33.0	37.0
32	35.43852265604837	37.0	37.0	37.0	33.0	37.0
33	35.436034915016776	37.0	37.0	37.0	33.0	37.0
34	35.422077203849135	37.0	37.0	37.0	33.0	37.0
35	35.35859326033256	37.0	37.0	37.0	33.0	37.0
36	35.38765346753678	37.0	37.0	37.0	33.0	37.0
37	35.39609740810382	37.0	37.0	37.0	33.0	37.0
38	35.38662666371714	37.0	37.0	37.0	33.0	37.0
39	35.27786104044538	37.0	37.0	37.0	33.0	37.0
40	35.08575010138996	37.0	37.0	37.0	33.0	37.0
41	35.252965195590455	37.0	37.0	37.0	33.0	37.0
42	35.30345094569185	37.0	37.0	37.0	33.0	37.0
43	34.97728864801091	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	38.0
16	65.0
17	34.0
18	46.0
19	46.0
20	71.0
21	202.0
22	546.0
23	1314.0
24	2612.0
25	4576.0
26	7479.0
27	11102.0
28	15712.0
29	20665.0
30	26924.0
31	34160.0
32	43865.0
33	57486.0
34	83168.0
35	169189.0
36	605618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.693304575452565	19.859067949710578	13.607915791026064	24.83971168381079
2	16.264701544814365	21.160269881650258	38.522287357593186	24.052741215942188
3	18.177653651882167	26.61385171256867	29.534804409541714	25.673690226007444
4	12.80490727426907	17.65024149246027	38.596947240349515	30.947903992921137
5	14.429635364819527	36.52149467241824	34.83823692069461	14.210633042067618
6	30.45210706780224	38.89613980754341	17.142646462411975	13.509106662242376
7	27.372248645061386	31.695701065516353	23.022619179294328	17.909431110127937
8	25.022029274047856	35.47809976772481	20.192087895881723	19.30778306234561
9	26.259539873907755	15.410168491686024	19.68993105482432	38.64036057958191
10	15.397172141724734	27.620654057442025	33.40587324411016	23.576300556723076
11	34.56402315378092	23.236459831139623	23.69096338900564	18.508553626073812
12	23.48375917118313	25.19476090402979	29.767816981897283	21.553662942889797
13	28.505696272536223	21.798842310953802	25.64926446189581	24.046196954614167
14	22.482671533384952	20.522158315820523	27.16071231058511	29.834457840209417
15	24.260959333407072	28.063082992294362	23.986008184935294	23.689949489363272
16	23.450577001069203	27.816521033808943	24.719426317147807	24.013475647974044
17	23.067691627032406	27.0207019872433	25.877207536039528	24.03439884968477
18	24.032002359620986	26.647402573461637	26.7481473288353	22.572447738082072
19	25.3833462375106	25.90412196290971	26.65634332485344	22.056188474726245
20	24.780168122995246	25.75249788002802	26.654499870958226	22.812834126018508
21	23.685064336540943	26.465453674003612	26.759576742985658	23.089905246469787
22	23.525513401909816	26.205157983998824	26.74704125649818	23.522287357593186
23	23.312410131622606	26.130221583158203	27.210209047671718	23.34715923754747
24	23.58256829996682	26.56444714817682	26.19723113224938	23.655753419606977
25	23.714651771559193	26.26645282601482	26.920418095343436	23.09847730708255
26	23.705618847472625	26.50693138664602	26.64832430040925	23.13912546547211
27	23.686354754267597	26.29640895181212	26.414850864579876	23.602385429340412
28	23.22982339711684	26.208660546399738	26.960236699480145	23.601279357003282
29	24.27791910924308	26.10846882719463	26.270969288058104	23.342642775504185
30	23.969232754488807	26.007631899126203	26.68832724993548	23.334808096449507
31	23.84479961656159	26.015005714707073	26.638369649375072	23.501825019356264
32	23.025292187442393	26.03187331784832	26.992773660730744	23.950060833978544
33	23.059027393724882	26.105519300962282	27.26956826309774	23.565885042215093
34	23.73363934667994	25.74226671090956	26.958946281753494	23.565147660657008
35	23.466154186483795	26.350329978247245	26.666390148582387	23.517125686686576
36	23.98139955019725	26.164786343693546	26.484625594513883	23.369188511595326
37	23.713730044611584	25.77968882498249	26.611731740589168	23.89484938981676
38	23.665155034472587	26.275854440880437	26.436327102459167	23.622663422187813
39	23.27978099767725	26.104505401319912	26.750451646204326	23.865261954798513
40	23.52237953028795	26.36323415551377	27.006323046860597	23.108063267337684
41	23.450484828374442	25.796187737344688	26.968624414703392	23.78470301957748
42	23.10234856026251	26.598458872543596	26.850643365409432	23.448549201784463
43	23.67280536813774	25.766968993105483	26.783449470928733	23.776776167828043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	36.0
2	54.0
3	194.0
4	334.0
5	334.0
6	404.0
7	474.0
8	462.0
9	450.0
10	602.0
11	754.0
12	754.0
13	1217.0
14	1680.0
15	3155.5
16	4631.0
17	5381.0
18	6131.0
19	6131.0
20	5778.5
21	5426.0
22	6052.5
23	6679.0
24	8947.5
25	11216.0
26	11216.0
27	15146.0
28	19076.0
29	23357.0
30	27638.0
31	32836.5
32	38035.0
33	38035.0
34	45705.0
35	53375.0
36	58243.5
37	63112.0
38	68206.5
39	73301.0
40	73301.0
41	76671.5
42	80042.0
43	81788.0
44	83534.0
45	83823.5
46	84113.0
47	84113.0
48	82489.0
49	80865.0
50	81774.5
51	82684.0
52	79194.5
53	75705.0
54	75705.0
55	72652.0
56	69599.0
57	62504.5
58	55410.0
59	51631.5
60	47853.0
61	47853.0
62	42307.5
63	36762.0
64	31336.0
65	25910.0
66	22183.5
67	18457.0
68	18457.0
69	15384.0
70	12311.0
71	10150.0
72	7989.0
73	6510.0
74	5031.0
75	5031.0
76	3836.0
77	2641.0
78	2077.0
79	1513.0
80	1206.5
81	900.0
82	900.0
83	723.0
84	546.0
85	454.0
86	362.0
87	273.0
88	184.0
89	184.0
90	141.5
91	99.0
92	60.5
93	22.0
94	13.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1084920.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.970306807149214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.93493405372992	53.48378856008359
2	9.105882491808998	11.468004285181221
3	2.401749541710929	4.537167165464017
4	1.0620159227517076	2.675018739590109
5	0.5618690748353565	1.7690534013915742
6	0.3542460707613571	1.338419025664185
7	0.25460367863824274	1.1222730230655278
8	0.18746164491279224	0.9443613835785112
9	0.14295672181464042	0.810182576953097
>10	0.8550426082906567	10.194941874990485
>50	0.08158265095664888	3.5907601345264406
>100	0.05411658229247016	6.1708827567592905
>500	0.0030965878093266387	1.1959575843726025
>1k	4.4236968704666264E-4	0.6991894883793243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3606	0.3323747373078199	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2525	0.23273605427128266	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1399	0.12894959997050473	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.8434538952180805E-4	0.0	0.0
2	0.0	0.0	3.686907790436161E-4	0.0	0.0
3	0.0	0.0	4.6086347380452014E-4	0.0	0.0
4	0.0	0.0	4.6086347380452014E-4	0.0	0.0
5	0.0	0.0	6.452088633263282E-4	0.0	0.0
6	0.0	0.0	9.217269476090403E-4	0.0	0.0
7	0.0	0.0	0.002857353537588025	1.8434538952180805E-4	0.0
8	9.217269476090402E-5	0.0	0.003318217011392545	1.8434538952180805E-4	0.0
9	9.217269476090402E-5	0.0	0.0038712531799579694	8.295542528481362E-4	0.0
10	9.217269476090402E-5	0.0	0.004055598569479777	9.217269476090403E-4	0.0
11	9.217269476090402E-5	0.0	0.0069129521070678025	0.0011060723371308485	0.0
12	9.217269476090402E-5	0.0	0.007465988275633226	0.0011982450318917524	0.0
13	9.217269476090402E-5	0.0	0.008019024444198651	0.0011982450318917524	0.0
14	9.217269476090402E-5	0.0	0.008387715223242267	0.0011982450318917524	0.0
15	9.217269476090402E-5	0.0	0.009493787560373114	0.0012904177266526563	0.0
16	9.217269476090402E-5	0.0	0.01050768720274306	0.0014747631161744644	0.0
17	9.217269476090402E-5	0.0	0.010876377981786676	0.0015669358109353685	0.0
18	9.217269476090402E-5	0.0	0.010968550676547579	0.0015669358109353685	0.0
19	9.217269476090402E-5	0.0	0.011060723371308484	0.0015669358109353685	0.0
20	9.217269476090402E-5	0.0	0.0114294141503521	0.0016591085056962725	0.0
21	9.217269476090402E-5	0.0	0.01189027762415662	0.0020277992847398886	0.0
22	9.217269476090402E-5	0.0	0.012258968403200236	0.0023964900637835048	0.0
23	9.217269476090402E-5	0.0	0.01235114109796114	0.002673008148066217	0.0
24	9.217269476090402E-5	0.0	0.012535486487482948	0.003041698927109833	0.0
25	9.217269476090402E-5	0.0	0.012535486487482948	0.003226044316631641	0.0
26	9.217269476090402E-5	0.0	0.012535486487482948	0.0038712531799579694	0.0
27	9.217269476090402E-5	0.0	0.012535486487482948	0.005069498211849721	0.0
28	9.217269476090402E-5	0.0	0.012535486487482948	0.012443313792722044	0.0
29	9.217269476090402E-5	0.0	0.012535486487482948	0.02829701729159754	0.0
30	9.217269476090402E-5	0.0	0.012719831877004756	0.050602809423736315	0.0
31	9.217269476090402E-5	0.0	0.012719831877004756	0.11576890461969547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2155	0.0	24.466358	1
GGTATCA	675	0.0	23.022223	1
TTCGCCG	205	0.0	20.756096	24
TACGACT	45	0.0038256713	20.555557	4
CGGCGTA	65	6.901672E-5	19.923077	12
GCGAACT	225	0.0	18.088888	28
CGAACTA	195	0.0	18.025642	29
GCCGGCA	270	0.0	17.814816	15
TTAACGG	230	0.0	17.695652	35
AGCTTCG	275	0.0	17.49091	21
TAACGGC	245	0.0	17.367346	36
TGCTCGC	295	0.0	16.305084	10
GGTTAAC	80	3.382347E-4	16.1875	1
TCAACGC	3205	0.0	16.162247	4
ATCAACG	3185	0.0	16.147568	3
TGCGGTA	150	4.669346E-9	16.033333	36
AACGCAG	3285	0.0	15.768646	6
CAACGCA	3280	0.0	15.679877	5
TATCAAC	3315	0.0	15.570136	2
CCGCTCT	275	0.0	15.472728	28
>>END_MODULE